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Development of a method to improve ischemia due to angiogenesis introducing from bypass grafts activating autologous bFGF at CABG

开发一种改善缺血的方法，该方法是通过在 CABG 中激活自体 bFGF 的旁路移植物引入血管生成而引起的

基本信息

批准号：
09670844
负责人：
KUSUMOTO Miyako
金额：
$ 1.98万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670844/
关键词：
ischemia coronary bFGF angiogenesis collateral CABG graft circulation antiogenesis 側副血行心筋虚血内因性冠動脈

项目摘要

Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in wound healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Collateral formation in ischemic heart is induced by angiogenic factors including bFGF.We hypothesized that bypass grafts with autologous angiogenic factors could induce angiogenesis and small capillaries will work as collateral circulation. Tissue-fragmented (TF-) grafts are rich of angiogenic activity, from shortly after implantation and its ability may work for collateral formation to the ischemic area when it is used for a small diameter vascular prosthesis in the coronary artery position. We developed a TF-graft and observed the healing process including angiogenesis and endothelialization. Proliferation of endothelial cells with capillary formation were active in the graft wall 5 days after implantation in a canine study. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for angiogenesis in vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have grafts with active angiogenesis for coronary revascularization, if we could have fine basic small-diameter fabric prosthesis with good handling characteristics, excellent antithrombogehicity.

碱性成纤维细胞生长因子（bFGF）是最强的血管生成剂之一，并且影响参与伤口愈合的所有细胞类型。bFGF分布广泛，因为它在调节细胞增殖和有丝分裂中起重要作用。缺血心脏侧支循环的形成是由包括bFGF在内的血管生成因子诱导的，我们假设含有自体血管生成因子的旁路移植物可以诱导血管生成，小毛细血管将作为侧支循环。组织碎片（TF-）移植物在植入后不久就具有丰富的血管生成活性，当其用于冠状动脉位置中的小直径血管假体时，其能力可用于缺血区域的侧支形成。我们研制了一种TF-移植物，并观察了愈合过程，包括血管生成和内皮化。在一项犬研究中，植入后5天，移植物壁中的内皮细胞增殖和毛细血管形成活跃。在第7天，内膜的整个厚度被组织化。这些结果表明，内源性碱性成纤维细胞生长因子是重要的血管假体的血管生成，而血栓和感染可能有负面影响。如果我们能有良好的操作特性、良好的抗血栓形成性、优良的小直径织物基片假体，就有可能获得具有活跃血管生成的冠状动脉血管重建术。