Effect to angiogenesis due to implantation of autologous tissue and fibroblasts in ischemic heart

自体组织和成纤维细胞植入缺血心脏对血管生成的影响

• 批准号: 11670712
• 负责人: KUSUMOTO Miyako
• 金额: $ 2.24万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670712/
• 关键词: Autologous tissue; incubation; implantation; angiogenesis; ischemic heart disease; fibroblast; capillaries; bFGF; angrogenesis; 虚血部; autologous; 組織; REC; 家兎; 末梢血管

From outside the body, the rabbit ear chamber (REC) provides a unique system for viewing capillaries in vivo. Most chambers are made of transparent easy-reflect materials including glass, acrylic resin and mica. The quality of macroscopic pictures of capillaries in the REC has not been satisfactory. With an illuminator from behind, flare was common, and with a flash light, reflection occurred. To obtain clear macroscopic pictures of REC with good contrast and sharp edges without flare, we made a new system. We used a Nikon F4s with a Medical Nikkor on a Nikon Multi photo stand. In a black light box, a strobe, Nikon Macro Speed Light SB-21, and a Modeling lamp were loaded. A 1-cm diameter hole was made at the edge on the top of the light box. Filming was done without difficulty. Without anesthetizing or stressing the animal, pictures were taken for one or two minutes. Without treatment, angiogenesis was observed at day 7 and vasculature was complete at 4 weeks in the REC field. The quality of pictures was satisfactory with contrast and sharp edges without flare up to 6 months. Our results suggest that this system is excellent to obtain direct evidence of angiogenesis in vivo from immediately after at weekly intervals over a long period.

从体外，兔耳腔（REC）提供了一个独特的系统来观察体内的毛细血管。大多数房间由透明易反射材料制成，包括玻璃、丙烯酸树脂和云母。REC中毛细血管的宏观图像质量并不令人满意。有了后面的照明器，耀斑很常见，有了闪光灯，就会发生反射。为了获得清晰、对比度好、边缘锐利、无耀斑的REC宏观图像，我们制作了一套新的系统。我们在尼康Multi照相架上使用了尼康f4和医用尼克尔。在一个黑色的灯箱里，装上了闪光灯、尼康微距测速灯SB-21和造型灯。在灯箱顶部边缘做一个直径1cm的孔。拍摄工作毫无困难。在没有麻醉或给动物施加压力的情况下，拍摄了一到两分钟的照片。在没有治疗的情况下，在第7天观察到血管生成，在第4周在REC领域血管系统完全完成。图像质量令人满意，对比度高，边缘锐利，6个月无耀斑。我们的研究结果表明，该系统非常适合在很长一段时间内每隔一周立即获得体内血管生成的直接证据。

项目成果

Tomizawa Y, Endo M, Nishida H, Niinami H, Tanaka S, Tomioka H, Ozawa H, Kikuchi C, Koyanagi H.: "Use of arterial grafts for coronary revascularization. Experience of 2987 anastomoses."Jpn J Thorac Cardiovasc Surg. 47. 325 (1999)

Tomizawa Y、Endo M、Nishida H、Niinami H、Tanaka S、Tomioka H、Ozawa H、Kikuchi C、Koyanagi H.：“使用动脉移植物进行冠状动脉血运重建。2987 例吻合术的经验。”Jpn J Thorac Cardiovasc Surg。