Effect to angiogenesis due to implantation of autologous tissue and fibroblasts in ischemic heart

从体外，兔耳腔（REC）提供了一个独特的系统，用于观察体内毛细血管。大多数腔室由透明易反射材料制成，包括玻璃、丙烯酸树脂和云母。REC中毛细血管的宏观图像质量不令人满意。如果使用后面的照明器，耀斑很常见，如果使用闪光灯，则会发生反射。为了获得清晰、对比度好、边缘清晰、无眩光的REC宏观图像，我们研制了一套新的系统。我们在尼康多功能摄影架上使用了尼康F4和医用尼克尔。在黑色灯箱中，装有闪光灯，尼康Macro Speed Light SB-21和造型灯。在灯箱顶部的边缘处制作一个直径为1 cm的孔。拍摄没有困难。在不麻醉或压迫动物的情况下，拍摄照片1或2分钟。在没有处理的情况下，在REC场中，在第7天观察到血管生成，并且在第4周血管系统完成。图片质量令人满意，对比度和锐利边缘无耀斑长达6个月。我们的研究结果表明，该系统是优秀的，以获得直接的证据，在体内血管生成后，立即在每周的时间间隔，在很长一段时间。

From outside the body, the rabbit ear chamber (REC) provides a unique system for viewing capillaries in vivo. Most chambers are made of transparent easy-reflect materials including glass, acrylic resin and mica. The quality of macroscopic pictures of capillaries in the REC has not been satisfactory. With an illuminator from behind, flare was common, and with a flash light, reflection occurred. To obtain clear macroscopic pictures of REC with good contrast and sharp edges without flare, we made a new system. We used a Nikon F4s with a Medical Nikkor on a Nikon Multi photo stand. In a black light box, a strobe, Nikon Macro Speed Light SB-21, and a Modeling lamp were loaded. A 1-cm diameter hole was made at the edge on the top of the light box. Filming was done without difficulty. Without anesthetizing or stressing the animal, pictures were taken for one or two minutes. Without treatment, angiogenesis was observed at day 7 and vasculature was complete at 4 weeks in the REC field. The quality of pictures was satisfactory with contrast and sharp edges without flare up to 6 months. Our results suggest that this system is excellent to obtain direct evidence of angiogenesis in vivo from immediately after at weekly intervals over a long period.