Making and analysis of a transjenic model of Mastocytosis and urticalia pigmentosa by the forced expression of steel factor in the skin.

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Transgenic mouse models for mastocytosis and urticalia pigmentosa were made by the forced expression of steel factor in the skin and the following results were obtained.1. Mastocytosis was reproducibly expressed by the expression of soluble steel factor in the basal cells of skin. Membrane-bound type steel factor developed only melanosis, but not any signs of mastocytosis.2. The mice that had shown mastocytosis were all developed melanocyte overgrowth in the skin, representing the symptom of urticalia pigmentosa, most common types of mastocytosis.3. In vivo administration of blocking antibody of steel factor-c-Kit signaling completely abolished the maintenance of mast cells and melanocytes, indicating that increase of these cells were caused by the expression of the steel transgene.4. Our mastocytosis models showed quicker and more extensive inflammatory response. This further implies that the model mice will be useful for the study of the roles of mast cells in inflammation.