The expression and the role of autocrine growth factors in differentiation of epidermal keratinocytes

自分泌生长因子在表皮角质形成细胞分化中的表达及其作用

• 批准号: 09670879
• 负责人: HIGASHIYAMA Mari
• 金额: $ 1.86万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670879/
• 关键词: Epidermal Keratinocyte; Cell Differntiation; Autocrine Growth Factor; HB-EGF; TGF-alpha; Involucrin; Keratin 5 and 14; Keratin 1 and 10; インポルクリン; ケラチン5、14; ケラチン1、10

The growth of keratinocytes is mediated by a variety of growth factors.As far as the EGF family is concerned, TGF-alpha, amphiregulin and HB-EGF have been reported to function as autocrine growth factors for keratinocytes.The growth of epidermal keratinocytes is associated with cell differentiation.It is unclear how autocrine growth factors operate in keratinocytes differentiation.In this study immunoreactive HB-EGF was detected in the epidermis of normal human and psoriatic skin.Immnunoreactive HB-EGF in paraffin embedded and formalin fixed tissues were primarily intercellular in basal keratinocytes with a progressive increase in their cytoplasmic localization as the keratinocyies became more differentiated.

角质形成细胞的生长是由多种生长因子介导的。就EGF家族而言，TGF-α，双调蛋白和HB-EGF已被报道作为角质形成细胞的自分泌生长因子。表皮角质形成细胞的生长与细胞分化有关。目前还不清楚自分泌生长因子在角质形成细胞分化中的作用。在这项研究中，免疫反应性HB-EGF正常人和银屑病患者表皮中均可检测到EGF，石蜡包埋和福尔马林固定组织中HB-EGF主要分布于基底层角质形成细胞的胞浆内，随着角质形成细胞的分化，HB-EGF的胞浆定位逐渐增多。

项目成果

Shigeki Inui,Shigeki Higashiyama,Koji hashimoto,Mari Higashiyama et al: "Possible role of coexpression of CD9 with membrane-anchored Heparin-binding EGF-like growth factor and amphiregulin in cultured human keratinocyte growth" Journal of cellular physiol

Shigeki Inui、Shigeki Higashiyama、Koji hashimoto、Mari Higashiyama 等人：“CD9 与膜锚定肝素结合 EGF 样生长因子和双调蛋白在培养的人角质形成细胞生长中的共表达的可能作用”细胞生理学杂志

Shigeki Inui, Shigeki Higashiyama, Koji hashimoto, Mari Higashiyama: "Possible role of coexpression of CD9 with membrane-anchored Heparin-binding EGF-like growth factor and amphiregulin in culured human keratinocyte growth" Journal of cellular physiology.

Shigeki Inui、Shigeki Higashiyama、Koji hashimoto、Mari Higashiyama：“CD9 与膜锚定肝素结合 EGF 样生长因子和双调蛋白在培养的人角质形成细胞生长中的共表达的可能作用”细胞生理学杂志。

Shigeki Inui, Shigeki Higashiyama, Koji hashimoto, Mari Higashiyama et al: "Possible role of coexpression of CD9 with membrane-anchored Heparin-binding EGF-like growth factor and amphiregulin in cultured human keratinocyte growth" Journal of cellular phys

Shigeki Inui、Shigeki Higashiyama、Koji hashimoto、Mari Higashiyama 等人：“CD9 与膜锚定肝素结合 EGF 样生长因子和双调蛋白在培养的人角质形成细胞生长中的共表达的可能作用”细胞物理学杂志

Shigeki Inui,Shigeki Higashiyama,Koji hashimoto,Mari Higashiyama: "Possible role of coexpression of CD9 with membrane-anchored Heparin-binding EGF-like growth factor and amphiregulin in cultured human keratinocyte growth" Journal of cellular physiology. 1

Shigeki Inui、Shigeki Higashiyama、Koji hashimoto、Mari Higashiyama：“CD9 与膜锚定肝素结合 EGF 样生长因子和双调蛋白在培养的人角质形成细胞生长中的共表达的可能作用”细胞生理学杂志。