Physiological role of oxytocin on androgen production in the testis.

催产素对睾丸雄激素产生的生理作用。

• 批准号: 09660342
• 负责人: SAWADA Tsutomu
• 金额: $ 2.11万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660342/
• 关键词: androgen; local hormone; male goat; male rat; oxytocin; prostaglandin F2 alpha; testis; testosterone; 5α-ジヒドロテストステロン; セルトリ細胞; 5α-dihydrotestosterone; Leydig cell; male rat; Oxytocin; prostaglandin F_2α; testis; testosterone; androgens

(1) The relationships among androgens (A), oxytocin (OT), and prostaglandin F2alpha(PGF2alpha), all of which are produced in Leydig cells, are not known. In this study, the effects of administration of OT on the production of A and PGF2alpha, or of PGF2alpha on the production of A and OT, in adult male rats were investigated. First, 100 ng of OT or 50*g of PGF2alpha was injected into a testis twice daily for 4 days. Some rats were injected with 10 IU of human chorionic gonadotropin (hCG) through the external jugular vein 1.5h before being sacrificed. hCG was given to stimulate the production of A.Rats were sacrificed and testicular hormone concentrations were measured. OT decreased T concentration and increased concentrations of 5alpha-dihydrotestosterone (DHT) and PGF2alpha. PGF2alpha decreased T concentration and tended to increase DHT concentration, but had no effect on OT.The injection of hCG increased the concentration of T and DHT, but the effects of OT or PGF2alpha on A producti … More on were similar whether hCG was injected or not. These results indicate that in adult rat testis, OT and PGF2alpha promote the metabolism of T, and suggest that the action of OT is mediated through PGF2alpha. (Prostaglandins & other Mediators 55 : 121-126, 1998)(2) Gene expression, immunohistochemical localization, binding, and effects of OT on A production in the testis of adult goats were studied. Using polymerase chain reaction (P CR) analysis we had been able to detect OT gene hanscripts in the goat testis. Immuno- histochemistry revealed that both OT and neurophysin epitopes were expressed together in the intratubular regions, especially in the Sertoli cells, suggesting the production of OT in these cells. However, enzyme immunoassay found no difference in OT concentration between testicular arterial plasma and venous plasma. Saturable, specific [3H]-OT binding sites were present in membrane fraction of the goat testis. Scatchard analysis indicated an apparent affinity of 42*7 L/nmol and binding capacity of 24*4 fmol OT bound/mg DNA.In vitro treatment of goat testes for 6 h with 100 nM OT led to a 3.5-fold increase (P<0.00l) in DHT production in spite of the induced decrease in testosterone, suggesting that OT not only affects testosterone production but also modulates its conversion to DHT.These results indicate that the goat testis produces OT, which may be involved in the local control of A biosynthesis. (Theriogenology : submitting) Less

(1)雄激素（A）、催产素（OT）和前列腺素F2 α（PGF 2 α）之间的关系尚不清楚，所有这些都是在Leydig细胞中产生的。在本研究中，研究了成年雄性大鼠给予OT对A和PGF 2 α产生的影响，或PGF 2 α对A和OT产生的影响。首先，将100 ng OT或50*g PGF 2 α注射到睾丸中，每天两次，持续4天。部分大鼠于处死前1.5h经颈外静脉注射人绒毛膜促性腺激素（hCG）10 IU。给予hCG以刺激A的产生。处死大鼠并测量睾丸激素浓度。OT降低T浓度，升高5 α-二氢睾酮（DHT）和PGF 2 α浓度。PGF 2 α降低T浓度，有升高DHT浓度的趋势，但对OT无影响，hCG使T和DHT浓度升高，而OT或PGF 2 α对A产生的影响与hCG的作用无关。 ...更多信息 无论是否注射hCG，均相似。以上结果表明，在成年大鼠睾丸中，OT和PGF 2 α促进T的代谢，提示OT的作用是通过PGF 2 α介导的。（Prostaglandins & other Mediators 55：121-126，1998）（2）研究了成年山羊睾丸中基因表达、免疫组织化学定位、结合以及OT对A产生的影响。应用聚合酶链反应（PCR）技术，在山羊睾丸组织中检测到OT基因的表达。免疫组化结果显示，OT和neurophysin表位在小管内区域，特别是在Sertoli细胞中同时表达，表明OT在这些细胞中产生。然而，酶免疫分析发现睾丸动脉血浆和静脉血浆之间的OT浓度没有差异。山羊睾丸膜部分存在饱和的特异性[~ 3 H]-OT结合位点。Scatchard分析表明，OT的表观亲和力为42 × 7 L/nmol，结合量为24 × 4fmol OT结合/mg DNA，100 nM OT体外处理山羊睾丸6 h，其结合量增加3.5倍（P<0.001），尽管诱导睾酮减少，表明OT不仅影响睾酮的产生，而且还调节其向DHT的转化。这些结果表明，山羊睾丸产生OT，其可能参与A生物合成的局部控制。（Theriogenology：submitting）减

项目成果

T.Sawada, K.Uemura, H.Tamada, T.Inaba, J.Mori: "Effects of oxytocin and prostaglandin F2 alpha on androgen production of adult rat testis in vivo." Prostaglandins & other Lipid Mediator. 55 (2-3). 121-126 (1998)

T.Sawada、K.Uemura、H.Tamada、T.Inaba、J.Mori：“催产素和前列腺素 F2 α 对成年大鼠睾丸体内雄激素产生的影响。”

T.sawada,K.Uemura,H.Tamada T.Inaba,J.Mori: "Effects of Oxytocin and prostaglandin F_2α on Androgen Production of Adult Rat Testis In Vivo." Prostaglandins & othey Lipid Mediators. 55・2-3. 121-126 (1998)

T.sawada、K.Uemura、H.Tamada T.Inaba、J.Mori：“催产素和前列腺素 F_2α 对成年大鼠睾丸体内雄激素产生的影响”55・2-3。 -126 (1998)