CLARIFICATION OF CONTROL MECHANISM OF NONSHIVERING THERMOGENESIS BY ANALYSIS OF GENE INFORMATION AND INTRACELLULAR SIGNAL TRANSDACTION SYSTEM

Brown adipose tissue （BAT），非生长热发生的主要位点，在代谢温度调节中发挥重要作用。目前的研究已经完成了澄清葡萄糖的作用及其在BAT功能上的控制机制，并取得了后续结果。（1）在Ca-treated大鼠中， Noradrenaline （NA）-诱导增加T-I-D2 BAT-D2和T-I-D2col-D2较小，可与控制大鼠相比。当T-D2尾部D2在Ca-treated大鼠中增加，但在控制下减少。这是一个建议，即Ca补充NA-induced thermogenesis在BAT中很好地作为外围血容器的构造。葡萄糖在治疗后的大鼠和控制剂之间的影响并不不同。（2）受感染的热耐受剂的食物摄入量，如良好的冷耐受剂， FOK大鼠并不因Std而产生差异：两种温暖附着和冷附着条件中的Wistar大鼠。（3）在冷吸收的FOK大鼠中，氧化物消费中的NA诱导增加比那些控制性物质要大。Glucagon诱导的增加在Tcols中也是在FOKRAT中更大的。（4）等离子体糖基剂水平在急性冷暴露后都比控制线更高，温接触和冷接触的FOK大鼠更高。（5） ECイD250エD2 of BAT to glucagon was smaller in FOK rat as compared to Std ： Wistar one。（6） Inositol-triphosphate（IPイイD23イエD2） increased oxygen consumption of BAT of neonatal rat dose-dependently。（7） Prolactin （PRL） treatment decreased the thermogenic response of BAT to glucagon。（8）BAT中PRL-受体mRNA的PCR分析演示信号。短形式PRL-受体mRNA的信号比长形式的信号更强，而这个信号在新生蝙蝠中比成人的信号更强。这些结果认为，Glucagon发挥了一个重要的作用，可以在抑制热发生器的非生长的热发生器中增强，如冷抑制剂、FOKRAT，and that glucagon stimulates BAT thermogenic activity via IPイイD23イイD2-CaイD12+イD1 route and this route is inhibitedly regulated by PRL.

Brown adipose tissue (BAT), the major site of nonshivering thermogenesis, plays important roles in metabolic temperature regulation. The present study was done to clarify the role of glucagon and its control mechanism on BAT function, and the following results were obtained.(1) In Ca-treated rat, noradrenaline (NA)-induced increases in TィイD2BATィエD2 and TィイD2colィエD2 were small as compared to control rat. While TィイD2tailィエD2 was increased in Ca-treated rat, but decreased in control by NA. It is suggested that Ca suppresses NA-induced thermogenesis in BAT as well as constriction of peripheral blood vessels. The effects of glucagon were not different between Ca-treated rat and control one.(2) Food intake of the inbred heat-tolerant, as well as cold-tolerant, FOK rat did not differ from that of Std : Wistar rat in both warm-acclimated and cold-acclimated conditions.(3) In cold-acclimated FOK rat, NA-induced increase in oxygen consumption was larger than those of control strains. Glucagon-induced increase in Tcol was also larger in FOK rat.(4) Plasma glucagon levels after acute cold exposure were higher in both warm-acclimated and cold-acclimated FOK rat than control strains.(5) ECィイD250ィエD2 of BAT to glucagon was smaller in FOK rat as compared to Std : Wistar one.(6) Inositol-triphosphate(IPィイD23ィエD2) increased oxygen consumption of BAT of neonatal rat dose-dependently.(7) Prolactin (PRL) treatment decreased the thermogenic response of BAT to glucagon.(8) PCR analysis demonstrated signals of PRL-receptor mRNA in BAT. The signal of short form PRL-receptor mRNA was stronger than that of long form one, and this signal was stronger in neonatal BAT than adult's one.These result suggest that glucagon plays an important role in enhansment of nonshivering thermogenesis of the inbred heat-tolerant, as well as cold-tolerant, FOK rat, and that glucagon stimulates BAT thermogenic activity via IPィイD23ィエD2-CaィイD12+ィエD1 route and this route is inhibitedly regulated by PRL.