Induction of plant phenolic amides by oligosaccharides

低聚糖诱导植物酚酰胺

• 批准号: 09660116
• 负责人: MIYAGAWA Hisashi
• 金额: $ 1.86万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660116/
• 关键词: potato; lminarin; p-coumaroyloctopamine; elicitor; active oxygen species; tyramine; tyrosine decarboxylase; tyramine hydroxycinnamoyl CoA transferase; オクトパミン; 活性酸素; 植物のシグナル伝達

A phenolic amide compound, p-coumaroyloctopamine (PCO), is induced in potato tuber disks by the treatment with beta-1,3-oligoglucosaccharide elicitor. The induction was observed in every potato cultivar collected in this study. Octopamine moiety of PCO was shown to be derived from tyrosine by the incorporation experiments using deuterium-labelled compounds. The level of free tyramine in the tuber tissue increased after the elicitor treatment, while no significant increase was observed regarding the octopamine level. Thus it was proposed that tyrosine is decarboxylated to tyramine, to which p-coumaroyl moiety is transferred from the corresponding CoA thioester, and the subsequent oxidation gives PCO in elicitor-treated potato tissue. The induction of PCO was thought to occur as a result of the activation of de novo synthesis caused by the oligosaccharide elicitor. The elicitor treatment coordinately raised the activities of tyrosine decarboxylase and tyramine hydroxycinnamoyl CoA transferase, the enzymes responsible for the formation of the octopamine moiety of PCO, together with those involved with phenylpropanoid metabolism including phenylalanine ammonia lyase and 4-hydroxycinnamic acid : CoA ligase. When the elicitor was applied in the presence of tiron or n-propyl gallate, the scavenger of active oxygen species, the induction of PCO was inhibited. The induction was also inhibited by diphenylene iodide, . an inhibitor of membrane-bound NADPH oxidase, suggesting that the active oxygen species produced by NADPH oxidase are involved with the signal transduction from the elicitor stimulation to the activation of PCO biosynthesis in potato tuber tissues.

通过用 β-1,3-低聚葡萄糖激发子处理，在马铃薯块茎中诱导产生一种酚酰胺化合物，对香豆酰基多巴胺 (PCO)。在本研究中收集的每个马铃薯品种中都观察到了诱导作用。通过使用氘标记化合物的掺入实验，表明 PCO 的章鱼胺部分衍生自酪氨酸。诱导剂处理后块茎组织中游离酪胺的水平增加，而章鱼胺水平没有观察到显着增加。因此，有人提出，酪氨酸脱羧为酪胺，对香豆酰基部分从相应的 CoA 硫酯转移到酪胺，随后的氧化在激发子处理的马铃薯组织中产生 PCO。 PCO 的诱导被认为是寡糖引发子引起的从头合成激活的结果。引发剂处理协调提高了酪氨酸脱羧酶和酪胺羟基肉桂酰辅酶A转移酶的活性，这些酶负责形成PCO的章鱼胺部分，以及与苯丙素代谢相关的酶，包括苯丙氨酸解氨酶和4-羟基肉桂酸辅酶A连接酶。当在钛试剂或没食子酸正丙酯（活性氧清除剂）存在的情况下使用引发剂时，PCO 的诱导受到抑制。二亚苯基碘也抑制诱导。一种膜结合 NADPH 氧化酶的抑制剂，表明 NADPH 氧化酶产生的活性氧参与了马铃薯块茎组织中从激发子刺激到 PCO 生物合成激活的信号转导。

项目成果

宮川 恒: "Induction of N-p-Coumaroyloctopamine in Potato Tuber Disks by β-1,3-Glucooligosaccharide" J.Pesticide Sci.23巻1号. 49-53 (1998)

Hisashi Miyakawa：“β-1,3-低聚葡萄糖在马铃薯块茎中诱导 N-p-香豆酰多巴胺”J.Pesticide Sci，第 23 卷，第 1. 49-53 期（1998 年）

H.Miyagawa, A.Ishihara, C.-H.Lim, T.Ueno and N.Furuichi: "Induction of N-p-coumaroyloctopaine in potato tuber disks by beta-1,3-glucooligosaccharide" J.Pesticide Sci.23. 49-53 (1998)

H.Miyakawa、A.Ishihara、C.-H.Lim、T.Ueno 和 N.Furuichi：“通过 β-1,3-低聚葡萄糖在马铃薯块茎中诱导 N-p-香豆酰氯托巴因”J.Pesticide Sci.23。

Miyagawa, H. 他: "Induction of N-p-Coumaroyloctopamine in Potato Tuber Disks by β-1, 3-Glucooligosaccharide" J.Pesticide Sci.23巻1号. 49-53 (1998)

Miyakawa, H. 等人：“β-1, 3-低聚葡萄糖在马铃薯块茎中诱导 N-p-香豆酰基多巴胺”，J.Pesticide Sci，第 23 卷，第 1. 49-53 期（1998 年）。