Exploration of chemical probes for investigation of oxidative metabolism of auxins

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Indole-3-acetic acid is a class of plant hormones, which is responsible for plant growth regulation. The concentration of IAA is strictly controlled by metabolism and transport. In IAA metabolism, oxidation plays an important role, but its mechanisms are not fully understood. In this study, we developed a screening system for inhibitors of IAA oxidation. As a result, we could establish a high-throughput screening system using LC/MS/MS to detect oxidative metabolites and evaluated the action of the known inhibitor of IAA oxidation in rice.