Development for functions of anthocyanins as foodstuffs useful for prevention oxidative in vivo and function mechanism

以百草枯诱导的氧化应激大鼠为模型，研究了茄子花青素、红萝卜花青素和蓝莓花青素对百草枯氧化应激大鼠的预防作用。在体内，那苏宁表现出较强的抗氧化作用，并能保护百草枯诱导的大鼠摄食量、体重增加、肝脏三甘油水平和线粒体过氧化氢酶活性降低，肺重量增加，NADPH-细胞色素P-450还原酶活性增加。以上结果提示，Nasunin对体内氧化应激具有保护作用。另一方面，虽然丹参和蓝莓中的花青素也表现出抑制摄食量减少、体重增加、肝脏线粒体过氧化氢酶活性下降和肺重量增加的趋势，但它们对氧化应激的预防作用弱于Nasunin。其体外清除超氧阴离子的能力强于花色苷和蓝莓，提示其体内较强的抗氧化活性部分是由于体内对自由基的清除作用所致。饲喂花青素抑制NADPH-细胞色素P-450还原酶活性，提示饲喂花青素的大鼠体内活性氧物种的产生可能受到抑制，从而减轻百草枯的氧化应激。

Preventive effects of nasunin, an anthocyanin in eggplant, and of anthocyanins in atsumi-kabu (a species of red turnip) and blueberries were examined in paraquat-induced oxidative stress rats.Nasunin showed strong antioxidative effect in vivo, and protected decreases in food intake , body weight gain, liver triacyiglycerol level and catalase activity in mitochondrial fraction, and increases in lung weight, and NADPH-cytochrome P-450 reductase activity which were induced by administering paraquat to rats. It was suggested from these results that nasunin is effective to protect oxidative stress in vivo. On the other hand, although anthocyanins in atsumi-kabu and blueberries also showed tendencies to suppress decreases in food intake, body weight gain, decrease in catalase activity in liver mitochondrial fraction, and increase in lung weight, their preventive effects for oxidative stress were weak than those of nasunin. Superoxide anion scavenging activity of nasunin in vitro was stonger than that of anthocyanins of atsumi-kabu and blueberries, suggesting that strong antioxidative activity of nasunin in vivo partly is due to radical scavenging activity of nasunin in vivo. inhibition of NADPH-cytochrome P-450 reductase activity which increase in administration of paraquat, by feeding anthocyanins suggested that generation of active oxygen species may be suppressed in rats fed on anthocyanins, resulting in relieve of oxidative stress by paraquat.