Development of anti-disease foodstuffs by use of a new radical scavenging system, and their verification and application in vivo

研究了XYZ体系中Y组分的抗氧化活性与化学发光强度的关系，其中X、Y、Z分别为活性氧、抗氧化剂（催化剂）和受体。从食品和生物系统中寻找Z物种的化合物。此外，还研究了钇的化学发光强度与其体内抗氧化活性的关系。另一方面，确定了被发现具有抑制癌症作用的Y或Z的活性，当这些组分单独使用或与用于预防人乳腺癌的药物组合使用时，这些组分是否显示出对乳腺癌细胞的抗增殖活性。研究结果如下：1. Y组分在脂质过氧化体系中表现出较强的抗氧化活性，在XYZ体系中也表现出较强的化学发光。结果表明，XYZ体系适用于食品中抗氧化物质的测定.（-）-表儿茶素没食子酸酯（ECg）有效地防止百草枯诱导的大鼠氧化应激，与（-）-表儿茶素的作用相比，其显示比EGg弱的化学发光。这一结果也表明XYZ系统可用于检测有效防止体内氧化应激的Y.血红蛋白细胞色素C过氧化物酶超氧化物歧化酶。和过氧化氢酶在体外更易引起脂质过氧化反应，在典型Y组分存在的情况下充当典型Z组分.味噌和鹰嘴豆芽素A单独或与预防乳腺癌的药物联合给药对大鼠移植的乳腺癌细胞具有抗增殖作用。

The relationship between antioxidative activity of Y component in XYZ system and intensity of chemiluminescence, in which X, Y and Z are active oxygen species, antioxidants (catalytic species) and receptors. Compounds act as Z species were also searched from foodstuffs and biological system. In addition the relationship between intensity of chemiluminescence of Y and its antioxidative activity in vivo were examined. On the other hand, activity of Y or Z, which was found to act to suppress cancer, was determined whether these components show antiproliferative activity for mammary cancer cell when were used alone or in combination with drug useful for prevention human breast cancer. The results were as follows.1. Y component which shows strong antioxidative activity in lipid peroxidation system, showed strong chemiluminescence in XYZ system, too. These results showed that XYZ system are useful to determine antioxidative substances from foodstuffs.2. (-)-Epicatechin gallate (ECg) prevented effectively paraquat-induced oxidative stress in rats when compared to the effect of (-)-epicatechin, which shows weak chemiluminescence than EGg. This result also showed that XYZ system is useful to examine Y which is effective to prevent oxidative stress in vivo.3. Hemoglobin, cytochrome C, peroxidase, superoxide dismutase. and catalase which are rather induce lipid peroxidation in vitro, acted as typical Z component in the presence of typical Y component.4. Miso and biochanin A in miso demonstrated antiproliferative effect when they were given to rats trasnplanted mammary cancer cells alone or in combination with drug useful for mammary cancer prevention.