Studies on biodegration mechanism of cellulose by white-rotting fungi.

白腐真菌生物降解纤维素机理的研究

基本信息

  • 批准号:
    09660176
  • 负责人:
  • 金额:
    $ 2.18万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1997
  • 资助国家:
    日本
  • 起止时间:
    1997 至 1998
  • 项目状态:
    已结题

项目摘要

The white wood-rotting fungus Phanerochaete chrysosporium grown on cellulose produces the oxidoreductase cellobiose dehydrogenase (CDH). In this study, the role of CDH in cellulose degradation was investigated.Adsorption of CDH is occurred only on the amorphous surface of cellulose, but not on the crystalline surface, suggesting that the function of CDH is not directly responsible for decomposition of the crystalline structure of cellulose.In the static cultures of P.chrysosporium grown on cellulose, most of CDH activity is observed in the mycelium-cellulose complex, but not released in the culture solution. Moreover, localization of CDH on the cellulose surface is specialized to the crack formed by the action of cellulases. This observation is a good evicdence to suggest the cooperative function between cellulases and CDH in vivo.The optimization of cultivation condition has been elaborated to obtain a large amount of CDH.The addition of calf bovine serum in the culture medium is resulted in the significant enhancement of CDH production in the extracellular solution. To investigated the reason of this phenomenon, the method using RT-PCR for measurement of the corresponding mRNA level have been established.The interaction between cellobiohydrolase I (CBH I) and CDH in the decomposition of the microcrystalline bacterial cellulose was investigated The result demonstrated that the CDH redox system enhances CBH I activity by relieving the product inhibition.The mechanism on pH dependence of electron transportation between flavin and heme cofactors in CDH was investigated. The result suggests that this phenomenon is caused by the pH depending conformational changes of the protein structure of the flavin containing domain.
生长在纤维素上的白色木腐真菌黄孢原毛革菌产生氧化还原酶纤维二糖脱氢酶(CDH)。本文研究了CDH在纤维素降解中的作用. CDH只吸附在纤维素的无定形表面上,而不吸附在纤维素的结晶表面上,表明CDH的功能不是直接分解纤维素的结晶结构.在纤维素上生长的黄孢原毛平革菌的静态培养中,CDH活性主要在菌丝体-纤维素复合体中观察到,但在培养液中不释放。此外,CDH在纤维素表面的定位是专门的纤维素酶的作用形成的裂纹。本研究通过优化培养条件,获得了大量的CDH,并在培养基中加入小牛血清,使细胞外液中CDH的产量显著提高。为了探究这一现象的原因,建立了RT-PCR检测相应mRNA水平的方法,研究了纤维二糖水解酶I(CBH I)结果表明,CDH氧化还原体系通过解除产物抑制而提高CBH Ⅰ的活性,并对pH依赖性的黄素与CDH之间的电子传递机理进行了探讨和血红素辅因子。结果表明,这一现象是由pH依赖性的蛋白质结构的构象变化的黄素含有域。

项目成果

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N.habu, K.Igarashi, M.Samejima, B.Pettersson, and K.-E.L.Eriksson :"Enhanced Production of Cellobiose Dehydrogenase in Cultures of Phanerochaete chrysosporium Supplemented with Bovine Calf Serum." Biotech.Appl.Biochem.26. 97-102 (1997)
N.habu、K.Igarashi、M.Samejima、B.Pettersson 和 K.-E.L.Eriksson:“补充了小牛血清的黄孢原毛平革菌培养物中纤维二糖脱氢酶的增强生产。”
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    0
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五十嵐圭日子: "セロビオース脱水素酵素とセルロース生分解におけるその役割" Cellulose Communication. 4. 56-60 (1997)
Keiichi Igarashi:“纤维二糖脱氢酶及其在纤维素生物降解中的作用”《纤维素通讯》,4. 56-60 (1997)。
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K.Igarashi: "Localization of Cellobiose dehydrogenase in cellulose-grown cultures of Phanerochaete chrysosporium" Fungal Genetic and Biolology. 21. 214-222 (1997)
K.Igarashi:“纤维二糖脱氢酶在黄孢原毛平革菌的纤维素生长培养物中的定位”真菌遗传学和生物学。
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K.Igarashi, M.F.J.M.Verhagen, M.Samejima, M.Schulein, K.-E.L.Eriksson, and T.Nishino :"Cellobiose dehydrogenase from Phanerochaete chrysosporium and Humicola insolens. : A flavohemoprotein from Humicola insolens contains 6-hydroxy-FAD as the dominant acti
K.Igarashi、M.F.J.M.Verhagen、M.Samejima、M.Schulein、K.-E.L.Eriksson 和 T.Nishino:“来自 Phanerochaete chrysosporium 和 Humicola insolens 的纤维二糖脱氢酶。:来自 Humicola insolens 的黄素血蛋白含有 6-羟基-FAD 作为
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M.Samejima: "The Behaviour of Phanerochaete chrysosporium cellobiose dehydrogenase on adsorption to crystalline and amorphous cellulose" Biotechnology and Applied Biochemistry. 25. 135-141 (1997)
M.Samejima:“黄孢原毛平革菌纤维二糖脱氢酶对结晶和无定形纤维素吸附的行为”生物技术和应用生物化学。
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SAMEJIMA Masahiro其他文献

SAMEJIMA Masahiro的其他文献

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{{ truncateString('SAMEJIMA Masahiro', 18)}}的其他基金

Establishment of a system model towards optimized utilization of woody biomass in Japan
日本木质生物质优化利用系统模型的建立
  • 批准号:
    24658153
  • 财政年份:
    2012
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Investigation on the ultrafine structure of wood cell wall for establishment of biorefinery technology of woody biomass
木质细胞壁超微结构研究建立木质生物质生物炼制技术
  • 批准号:
    23248025
  • 财政年份:
    2011
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Recognition and response mechanism on hemicellulose structure in the initial stage of wood degradation by basidiomycete fungi
担子菌对木材降解初期半纤维素结构的识别及响应机制
  • 批准号:
    20380100
  • 财政年份:
    2008
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation on function and gene expression of multiple cellulolytic enzymes from filamentous fungi.
丝状真菌多种纤维素分解酶的功能和基因表达研究。
  • 批准号:
    14360094
  • 财政年份:
    2002
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation on Function and Molecular Structure of Cellobiose Dehydrogenase
纤维二糖脱氢酶的功能和分子结构研究
  • 批准号:
    11660161
  • 财政年份:
    1999
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation on the degradation mechanism of crystalline cellulose by cellulases using a new sensitive detection method.
采用新型灵敏检测方法研究纤维素酶降解结晶纤维素的机制。
  • 批准号:
    06660205
  • 财政年份:
    1994
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
The role of Cellobiose Dehydrogenases in Cellulose Biodegradation by Filamentous Fungi
纤维二糖脱氢酶在丝状真菌纤维素生物降解中的作用
  • 批准号:
    04660176
  • 财政年份:
    1992
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Investigation on Function and Molecular Structure of Cellobiose Dehydrogenase
纤维二糖脱氢酶的功能和分子结构研究
  • 批准号:
    11660161
  • 财政年份:
    1999
  • 资助金额:
    $ 2.18万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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