Expression of liver specific functions in chicken hepatocytes

Rats primary hepatocytes have been usually cultured in a 5% coi D22 D2 incubator at ph7.4 .however,we found that chicken hepatocytes were able to cultured in serum-free L-15 medium for a long periodin free gas exchange with the atmosphere (without CO - D22 - D2 gas). the amount of albumin secretedin the medium decreased to low level earlyfollowed by marked increase from day 9 to day 17 of culture. The profile of appearance of albuminm-RNA是非常简单的，即albumin protein. Such observation was not observed in rathepatocytes,showing some difference in the properties of hepatocytes between avian and rat. Therefore，我们tried to monitored the effects of enbironmental pollution chemicals on metabolism in liver ofwild birds using chicken hepatocytes as a model. 10 d -1 d -1 M TCDD （telacholoro -dibenzo-p-dioxin）decreased the expression of albuminand 10 d -2 d - 1 M TCDD markedly induced the expression of the depoisoning enzyme, P450 CYP1A4。

Rats primary hepatocytes have been usually cultured in a 5% COィイD22ィエD2 incubator at pH 7.4. However, we found that chicken hepatocytes were able to cultured in serum-free L-15 medium for a long period in free gas exchange with the atmosphere (without COィイD22ィエD2 gas). The amount of albumin secreted in the medium decreased to low level early, followed by marked increase from day 9 to day 17 of culture. The profile of appearance of albumin m-RNA was very similar to that of albumin protein. Such observation was not observed in rat hepatocytes, showing some difference in the properties of hepatocytes between avian and rat. Therefore, we tried to monitored the effects of enbironmental pollution chemicals on metabolism in liver of wild birds using chicken hepatocytes as a model. 10ィイD1-1ィエD1 M TCDD (tetracholoro-dibenzo-p-dioxin) decreased the expression of albumin, and 10ィイD1-2ィエD1 M TCDD markedly induced the expression of the depoisoning enzyme, P450 CYP1A4.