High Sensitive Detection of Biochemical Substances Using by Thin-film Optical Waveguide Sensor
利用薄膜光波导传感器高灵敏度检测生化物质
基本信息
- 批准号:09650486
- 负责人:
- 金额:$ 2.11万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In order to measure concentration of biochemical substances and ion solutions with high sensitivity and wide range band, thin-film optical waveguide sensor was developed under the basis of theoretical consideration of optimal waveguide construction. Three layers slab type of optical waveguide was constructed by substrate (clad) layer, waveguide (core) layer and sample (clad) layer. Sensitivity of evanescent field absorption, complex propagation constant of the waveguiding and interaction ratio of the waveguide sensor to aqueous light-absorbing species, which are measure of device sensitivity, were theoretically and experimentally investigated. Pyrex glass plate (refractive index n=l.47) for the substrate layer and rf-sputtered Corning 7059 glass (n=1.58) for the waveguide layer were used respectively. Top clad layer was composecd by air layer and sample layer on which very small quantity of biochemical aqueous solution was droped. Principle of the optical sensing was based on the evane … More scent field absorption of biochemical molecules and ions at the specific light wavelength. Oxy- and deoxyhemoglobin concentration and glucose concentration with chemo-reactive enzyme were measured by using the evanescent field absorption method. Another type of waveguide having composite polymer (PVCPVA) core layer (n=l.51) was also developed by means of spin-coating method. In the composite polymer, ion selective ionophore and specific dye were doped, of which sensing mechanism was dependent on changing of light absorption of the doped medium when the objective ion was specifically combined with the ionophore. Concentration of Na, K or Ca ion was selectively detected by changing the respective ionophores. This type of sensor was functioned not by evanescent field absorption but by bulk absorption. One of limitations of conventional ATR spectrophotometry was poor sensitivity for weakly light absorbing samples. This arises from that the effective sample thickness and length are in the order of penetration depth and length of the evanescent wave. The proposed optical waveguide sensor gave enough high interaction length between the evanescent field and sample meidum and therefore it was available to high sensitivity and wide range of concentration measurement. In this study, a new method of short time measurement for ion concentration calculation was also proposed by using exponential response curve fitting. Less
为了实现对生化物质和离子溶液浓度的高灵敏度、宽频带测量,在对最佳波导结构进行理论分析的基础上,研制了薄膜光波导传感器。三层平板型光波导由基片(包层)、波导(芯层)和样品(包层)构成。理论和实验研究了作为器件灵敏度度量的倏逝场吸收灵敏度、波导的复传播常数和波导传感器与水溶液光吸收组分的相互作用比。分别使用Pyrex玻璃板(折射率n= 1.47)作为衬底层和RF溅射Corning 7059玻璃(n=1.58)作为波导层。上覆层由空气层和试样层组成,试样层上滴有极少量的生化水溶液。光学传感的原理是基于evane ...更多信息 在特定光波长下生物化学分子和离子的气味场吸收。氧和脱氧血红蛋白浓度和葡萄糖浓度与化学反应酶的测定,通过使用倏逝场吸收法。另一种类型的具有复合聚合物(PVCPVA)芯层(n= 1.51)的波导也通过旋涂方法开发。在复合聚合物中掺杂离子选择性离子载体和特异性染料,其传感机理依赖于目标离子与离子载体特异性结合时掺杂介质光吸收的变化。Na、K或Ca离子的浓度通过改变各自的离子载体来选择性地检测。这种类型的传感器的功能不是由倏逝场吸收,而是由体积吸收。传统ATR分光光度法的局限性之一是对弱光吸收样品的灵敏度低。这是由于有效样品厚度和长度与倏逝波的穿透深度和长度的数量级相同。该光波导传感器具有足够大的倏逝场与样品介质的相互作用长度,可用于高灵敏度和宽范围的浓度测量。本文还提出了一种利用指数响应曲线拟合的短时测量离子浓度计算方法。少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.H.Kim,H.Minamitani,他: "Active optical thin-film waveguide sensor for ion senseing" Analytica Chimica Acta. 343. 199-208 (1997)
K.H.Kim、H.Minamitani 等人:“用于离子传感的有源光学薄膜波导传感器”Analytica Chimica Acta 343. 199-208 (1997)
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Kyongho Kim, Haruyuki Minamitani他: "Thin film waveguide sensor for measurement of the absorption coefficient of hemoglobin derivatives" Optical Review. 5・4. 257-261 (1998)
Kyongho Kim、Haruyuki Minamitani 等:“用于测量血红蛋白衍生物吸收系数的薄膜波导传感器”光学评论 5・4 (1998)。
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Kunihiro Matsumoto, Haruyuki Minamitani, et.al: "Basic characteristics of optical thin-film waveguide sensor for biochemical substances" Trans.Inst.Electr.Inform.Comm.Eng.Vol.J81・D2, No.6. 1439-1447 (1998)
Kunihiro Matsumoto、Haruyuki Minamitani 等:“用于生化物质的光学薄膜波导传感器的基本特性” Trans.Inst.Electr.Inform.Comm.Eng.Vol.J81・D2,No.6。 1998)
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H.Hisamoto,H.Minamitani,他: "Ion-sensitive and selective active waveguide optodes" Analytica Chimica Acta. 342. 31-39 (1997)
H. Hisamoto、H. Minamitani 等人:“离子敏感和选择性有源波导光极”Analytica Chimica Acta 342. 31-39 (1997)
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- 影响因子:0
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Kyongho Kim, Haruyuki Minamitani他: "Sensing property of thin film optical waveguide sensor based on PVC co-polymer" Proc.SPIE(Int.Soc.Opt.Eng.). 3278. 1059-1072 (1998)
Kyongho Kim、Haruyuki Minamitani 等:“基于 PVC 共聚物的薄膜光波导传感器的传感特性”Proc.SPIE(Int.Soc.Opt.Eng.) 3278. 1059-1072 (1998)。
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MINAMITANI Haruyuki其他文献
MINAMITANI Haruyuki的其他文献
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{{ truncateString('MINAMITANI Haruyuki', 18)}}的其他基金
Study on cellular interactions of blood cells and endothelial cells under diabetic and ischemia/reperfusion dysfunctions using microcirculatory mock system
利用微循环模拟系统研究糖尿病和缺血/再灌注功能障碍下血细胞和内皮细胞的细胞相互作用
- 批准号:
19300163 - 财政年份:2007
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on vascular shutdown effects of carcinoma blood vessels and cytotoxic effects of carcinoma cells induced by photochemical reaction.
光化学反应诱导癌血管关闭效应及癌细胞细胞毒作用的研究。
- 批准号:
14380410 - 财政年份:2002
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Combined effect of reoxygenation and photodynamic therapy on the tumor treatment
复氧与光动力疗法联合治疗肿瘤
- 批准号:
12480267 - 财政年份:2000
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Micro-machining of microcirculation model and a method for analyzing deformability and fluidity of blood cells
微循环模型微加工及血细胞变形性和流动性分析方法
- 批准号:
11558112 - 财政年份:1999
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Multi-sensors system of physiological signals detected in oral cavity for health care monitoring
用于健康护理监测的口腔生理信号多传感器系统
- 批准号:
09558122 - 财政年份:1997
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of Easily Phase-Matchable Wavelength-Conversion Devices Due to Fabrication of Periodically Polarization-Inverted Structures Using Organic Nonlinear-Optical Crystals.
由于使用有机非线性光学晶体制造周期性偏振反转结构,开发了易于相位匹配的波长转换器件。
- 批准号:
05558116 - 财政年份:1993
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Multi-Joint Control and Functional Restoration System of Paralyzed Extremity Using Functional Electrical Stimulation
功能性电刺激瘫痪肢体多关节控制与功能恢复系统
- 批准号:
01550341 - 财政年份:1989
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Functional Electrical Stimulation System for Motion Control of Lower and Upper Extremities.
用于下肢和上肢运动控制的功能性电刺激系统。
- 批准号:
61550282 - 财政年份:1986
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)