Combined effect of reoxygenation and photodynamic therapy on the tumor treatment

复氧与光动力疗法联合治疗肿瘤

• 批准号: 12480267
• 负责人: MINAMITANI Haruyuki
• 金额: $ 9.34万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12480267/
• 关键词: tumor cell; oxygen transfer; radiation therapy; biomechanics; photodynamic therapy; micro oxygen electrode; 腫瘍組織; 再酸素化; 血管新生; 光感受性物質; 活性酸素

The efficient radiation therapy requires the increased oxygen tension and the photodynamic therapy (PDT) is achieved by photosensitizing drug and laser as excitation light for the tumor cells. The combined effect of these two treatments is expected for more efficient therapy. So we need to clarify the destructive ability by the radiation to the oxygenated tumor tissue and the condition of uptake of photosensitizing drug. The purpose of this study is to clarify the process of improved radiation sensitivity and the production of active oxygen induced by photochemical reaction. The fluorescence derived from a monocyte added Zinc coproporphyrin III; ZnCP-III before measurement and with HeNe laser irradiation caused fluorescence distribution move to increase as the time course. The result indicates that photochemical reaction occurred due to excitation of photpsensitizer and active oxygen derived from the reaction stimulated monocytes. The active monocytes generated active oxygen and H2O2 was made visibly by dichorofluorescin; DCFH fluorescence method. We confirmed that activated monocytes by photochemical reaction are concerned with vascular shut down effect.

有效的放射治疗需要增加氧分压，而光动力学治疗（PDT）是通过光敏药物和激光作为肿瘤细胞的激发光来实现的。这两种治疗方法的联合作用有望获得更有效的治疗。因此，我们需要明确放射线对氧合肿瘤组织的破坏能力和光敏药物的摄取情况。本研究的目的是阐明光化学反应诱导提高辐射敏感性和产生活性氧的过程。在测定前加入锌粪卟啉III（ZnCP-III）的单核细胞产生的荧光和HeNe激光照射引起的荧光分布随时间推移而增加。结果表明，光化学反应的发生是由于光敏剂的激发和反应刺激单核细胞产生的活性氧。DCFH荧光法检测单核细胞活性氧和H_2O_2的产生。我们证实了通过光化学反应激活的单核细胞与血管关闭效应有关。

项目成果

S.Kudo,R.Morigaki,J.Saito,M.Ikeda,K.Oka,K.Tanishita: "Shear stress effect on mitochondorial potential and albumin uptake in cultured endothelial cells"Biochemical & Biophysical Research Communications. 270. 616-621 (2000)

S.Kudo，R.Morigaki，J.Saito，M.Ikeda，K.Oka，K.Tanishita：“剪切应力对培养内皮细胞线粒体电位和白蛋白摄取的影响”生化

Y.Imazeki,T.Nagao,A.Saito,C.Oshio,E.Sekizuka,H.Minamitani: "Vascular shutdown effect in photodynamic therapy-Mechanism of platelet thrombus formation by photochemical reaction-"Microcirculation Annual. Vol.16. 69-70 (2000)

Y.Imazeki，T.Nagao，A.Saito，C.Oshio，E.Sekizuka，H.Minamitan：“光动力疗法中的血管关闭效应-光化学反应血小板血栓形成的机制-”微循环年鉴。

南谷晴之他: "光励起による臓器微小循環の血流動態および酸素分圧計測"電気学会論文誌C. Vol.121-C, No.9. 1415-1421 (2001)

Haruyuki Minamitani 等人：“通过光激励测量器官微循环中的血流动力学和氧分压”，日本电气工程师学会会刊 C. 第 121-C 卷，第 1415-1421 号（2001 年）。

Tomokazu Nagao, Kazuki Matsuzaki, Miho Takahashi, Yoshiharu Imazeki, Haruyuki Minamitani: "Quantitative analysis for intracel lular distribution of photosensitizer using confocal laser scanning microscope"IEICE Transactions on Information and Systems. Vol

Tomokazu Nagao、Kazuki Matsuzaki、Miho Takahashi、Yoshiharu Imazeki、Haruyuki Minamitani：“使用共焦激光扫描显微镜对光敏剂的细胞内分布进行定量分析”IEICE 信息与系统交易。

南谷晴之他: "Analysis of platelet thrombus formation mechanism by photodynamic therapy-Influence to VSD effect by leuckoytes"Microcirculation Annual. 93-94 (2001)

Haruyuki Minamitani等人：“光动力疗法对血小板血栓形成机制的分析-白细胞对VSD效应的影响”微循环年鉴93-94（2001）。