Combined effect of reoxygenation and photodynamic therapy on the tumor treatment

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The efficient radiation therapy requires the increased oxygen tension and the photodynamic therapy (PDT) is achieved by photosensitizing drug and laser as excitation light for the tumor cells. The combined effect of these two treatments is expected for more efficient therapy. So we need to clarify the destructive ability by the radiation to the oxygenated tumor tissue and the condition of uptake of photosensitizing drug. The purpose of this study is to clarify the process of improved radiation sensitivity and the production of active oxygen induced by photochemical reaction. The fluorescence derived from a monocyte added Zinc coproporphyrin III; ZnCP-III before measurement and with HeNe laser irradiation caused fluorescence distribution move to increase as the time course. The result indicates that photochemical reaction occurred due to excitation of photpsensitizer and active oxygen derived from the reaction stimulated monocytes. The active monocytes generated active oxygen and H2O2 was made visibly by dichorofluorescin; DCFH fluorescence method. We confirmed that activated monocytes by photochemical reaction are concerned with vascular shut down effect.