Disinfection of Cryptosporidium by ozone

臭氧消毒隐孢子虫

• 批准号: 09650611
• 负责人: KANEKO Mitsumi
• 金额: $ 1.34万
• 依托单位: Setsunan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650611/
• 关键词: Ozone; Cryptosporidium parvu; Oocyst; Ct value; disinfection; クリプトスポリジウム; オゾン消毒; 脱嚢試験; ct値; DAPI; PI染色試験

Waterbone outbreaks caused by the oocysts of Cryptosporidium which are resistant to many water treatment processes are increasingly reported. In order to examine the potential of ozone for activating Cryptosporidium parvum were conducted.The evaluation of disinfection wais distinctively affected by the method of judge merit of vitality of oocysts. The most reliable estimation on the survival number of of infective oocysts should be obtained by the method depended on infective activity of oocysts using living animals. The Ct values for 90%(llog) and 99%(21og90%) reduction of oocysts examined by the method of excystation were 7-8mg・min/iotaand l2mg・min/iotaA.respectively. The Ct values for oocyst inactivation judged by DAPI/PI staining method were 7-llmgmin/iota. and 20 mg・min/iota for the reductions of 90%(llog) and 99%(2log) respectively . When the Ct value of ozone was estimated using the results obtained by infectivity of oocysts to mice, the Ct value for 99%(2log) reduction was 6 mg・min/iota. The Ct value of chlorine estimated by infevtive activity was 1200 mg・minIL for 9O%(1log) reduction , indicating that the Ct value of chlorination was lessthan the values reported so far. However this value is not not enough to guarantee the safety of water quality against Cryptosporidium. It is concluded from this study that ozone can be used to obtain the safe water against Cryptosporidium taking the Ct value of around 10 mg・min/iota.

隐孢子虫卵囊对许多水处理工艺具有抗药性，引起水体暴发的报道越来越多。为了考察臭氧对微小隐孢子虫的激活潜力，用卵囊活力评价法对臭氧消毒效果的评价有显著影响。用活体动物进行感染性卵囊存活数的估计，应根据卵囊的感染活性进行。脱囊法检测90%（1 log）和99%（21 log）卵囊减少的Ct值分别为7- 8 mg·min/10和12 mg·min/10。用DAPI/PI染色法判断卵囊灭活的Ct值为7- 11 mgmin/μ l。20 mg·min/iota时，降低率分别为90%（1 log）和99%（2log）。当使用卵囊对小鼠的感染性获得的结果来估计臭氧的Ct值时，99%（2log）减少的Ct值为6 mg·min/iota。在90%（1 log）的去除率下，用感染活性估算的氯的Ct值为1200 mg·minIL，表明氯化的Ct值小于目前报道的值。但该值不足以保证水质对隐孢子虫的安全。结果表明，当Ct值在10 mg·min/iota左右时，臭氧可用于获得抗隐孢子虫的安全饮用水。

项目成果

Kaneko Mitsumi: "Preventive measures against pathogenic organism disaster in sewage (Cryptosporidium, E.coli, Virus, etc)" Proceedings 7thWEF/JSWA Joint Technical Seminar on SewageTreatment Technology. 151-177 (1998)

金子光美：《污水中病原生物灾害的预防措施（隐孢子虫、大肠杆菌、病毒等）》论文集第七届WEF/JSWA污水处理技术联合技术研讨会。

Ozawa Katsuyuki, Chikuma Daisuke, Hirata Tsuyosi, Motoyama Nobuyuki, Takahasi Kazutaka, Moniwa Takeo, Kaneko Mitsumi: "Inactivation of Cryptosporidium parvum oocysts with ozone-Evaluation by excystation and DAPI/PI staining-. (Japanese)" 32th Annual Confe

Ozawa Katsuyuki、Chikuma Daisuke、Hirata Tsuyosi、Motoyama Nobuyuki、Takahasi Kazutaka、Moniwa Takeo、Kaneko Mitsumi：“用臭氧灭活隐孢子虫卵囊 - 通过排泄和 DAPI/PI 染色进行评估 -（日语）”第 32 届年度会议

金子光美: "水の感染症リスクと震災対応" 水処理生物学会誌. 18. 3-4 (1998)

Mitsumi Kaneko：“水传染病的风险和地震灾害反应”日本水处理生物学会杂志 18. 3-4 (1998)。