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Extraction Rates and Plant Engineering Study on the Reversed Micellar Extraction of Protein Using Tank or Column Type Contactor

使用罐式或柱式接触器反胶束提取蛋白质的提取率和设备工程研究

基本信息

批准号：
09650850
负责人：
NISHIKI Tadaaki
金额：
$ 1.73万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

1. Extraction equilibria of amino acids by AOT reverse micelles were studied to obtain fundamental information on this research project. The effects of different factors on the partitioning behavior were clarified.2. The mass transfor rates in forward and back extractions of protein lysozyme or amino acid phenylalanine through a flat interface between an aqueous KCl solution and an AOT-is ooctane reversed micellar solution were investigated to elucid ate the mass transfer characterization in the reversed micellar extraction. The solubilization and release rates at the interface were evaluated.3. Forward and back extraction rates oflysozyme between aqueous KCl solutions and AOT-isooctane reversed micellar solutions were measured using a batch agitated tank to investigate a possibility of concentrating and recoverying a protei n rapidly by reversed micell ar extraction. The forward extraction was very fsst and lysozyme was quantitatively extracted to the organic phase for 1 min. The back extraction was slow compared to the forward extraction, but the aqueous lysozyme concentration reached to the equilibrium value within 5 min. It was therefore found that lysozyme could be concentrated and recovered rapidly. The time-dependent concen- tration oflysozyme could be simulated by a mass transfer model applying the extraction model through a flat interface.4. Forward and back extraction experiments of phenylalanine were carried out by using a agitated tank and a rotating disc column under various conditions. In the agitated tank there was no problem ir both iwward and back extractions, but in the column operation phenylalanine could not be smoothly recovered from the reversed micellar organic phase to the aqueous stripping phase. At the present time, therefore, the rotating disc column was inapplicable to the back extraction process, and the improvement of the contactor is the subject for a future study.

1.研究了AOT反胶团萃取氨基酸的平衡，为该研究项目提供了基础资料。阐明了不同因素对分配行为的影响.研究了AOT-正辛烷反胶束溶液与KCl水溶液在平界面上正、反萃取蛋白质、溶菌酶和氨基酸苯丙氨酸的传质速率，以阐明反胶束萃取的传质特性。考察了微球在界面的增溶和释放速率.采用间歇式搅拌槽，测定了溶菌酶在KCl水溶液和AOT-异辛烷反胶束溶液中的正、反萃取速率，探讨了反胶束萃取快速浓缩回收蛋白质的可能性。正向萃取非常快速，溶菌酶被定量萃取到有机相1 min。反向萃取与正向萃取相比缓慢，但溶菌酶水溶液浓度在5 min内达到平衡值。因此，发现溶菌酶可以快速浓缩和回收。应用平界面萃取模型建立的传质模型可以模拟酶液浓度随时间的变化.采用搅拌槽和转盘萃取柱在不同条件下对苯丙氨酸进行了正、反萃取实验。在搅拌槽中，逆萃取和反萃取都没有问题，但在柱操作中，苯丙氨酸不能顺利地从反胶束有机相回收到反萃水相中。因此，目前转盘塔不适用于反萃取工艺，对转盘塔的改进是今后研究的课题。