Physiological roles of insulin-like growth factor I in the mouse uterine endometrium.

雌激素和黄体酮调节子宫内膜细胞的生长。雌激素诱导小鼠子宫内膜细胞的增殖和分化可能与生长因子有关。本研究旨在确定胰岛素样生长因子（IGF-I）在原代无血清培养中是否能刺激小鼠上皮细胞和基质细胞的增殖。在上皮细胞和基质细胞中均检测到IGF-I mRNA和IGF-I受体（r型）mRNA。雌激素刺激了IGF-I的合成，雌激素与孕激素联合在基因转录水平刺激了IGF-I受体的合成。igf - 1以剂量依赖的方式刺激上皮细胞和基质细胞的增殖。这些研究共同表明，子宫内膜igf - 1以旁分泌或自分泌的方式调节子宫内膜细胞的生长，很可能是雌激素作用的中介。我们成功地在体外重建了子宫内膜。将基质细胞悬浮在胶原凝胶中，将上皮细胞镀在胶原凝胶中的基质细胞层上。上皮细胞中可见连接复合体结构（紧密连接和小带粘附）和微绒毛。上皮细胞与基质细胞共培养可增加上皮细胞的数量。这表明基质细胞影响上皮细胞的生长。上皮细胞与基质细胞相互作用的分子机制有待进一步研究。

Estrogens and progestin regulate the growth of uterine endometrial cells. Estrogen-induced proliferation and differentiation of mouse endometrial cells may be mediated by growth factors. This study was undertaken to determine whether insulin-like growth factor (IGF-I)' stimulates proliferation of mouse epithelial and stromal cells in primary serum-free culture. In both epithelial and stromal cells, IGF-I mRNA and IGF-I receptor (type r) mRNA were detected. Estrogen stimulated IGF-I synthesis, and combination of estrogen and progesterone stimulated IGF-I receptor synthesis at the level of gene transcription. IGF-I stimulated the proliferation of both the epithelial and stromal cells in a dose-dependent manner. These study altogether indicate that endometrial IGF-I regulates the growth of endometrial cells in a paracrine or autocnine manner, highly probably as mediator of estrogen actions.We successfully reconstituted the uterine endometrium in vitro. The stromal cells were suspended in collagen gel, and the epithelial cells were plated on the stromal cell layer in the collagen gel. Junctional complex structure (tight-junction and zonula adherence) and microvilli were observed in the epithelial cells. Co-culture of epithelial cells and stromal cells increased the number of epithelial cells cultured. This suggests that the stromal cells affect the growth of epithelial cells. Molecular mechanism of this interaction between the epithelial cells and stromal cells needs to be clarified.