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Analysis of the information network for protein synthesis and metabolism in neuron

神经元蛋白质合成代谢信息网络分析

基本信息

批准号：
09640806
负责人：
OZAKI Koichi
金额：
$ 2.37万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640806/
关键词：
vesicle transport Rab photoreceptor cell neuron rhodopsin endocytosis Drosophila Rab蛋白質ゴルジ体小胞体

项目摘要

In this project, the network system of vesicle transport and the communication between its elementary steps have been studied with special attention to Rab proteins by using Drosophila photoreceptor cell as a model cell for neuron. For this purpose, I investigated the tissue- and intracellular-distribution of Rab proteins, and carried out the biochemical and morphological analyses of the phenotypes of the dominant negative mutants of these Rab proteins. The results are as follows :1. Proteins localized in the photoreceptive membrane domain as well as those in the axonal domain are transported from the rER to the Golgi body under the control of RAB1.2. RAB2 would possibly control all pathways from the Golgi body to the photo-receptive membrane, the axonal membrane and the lysosome. This suggests that RAB2 would links between these pathways.3. RAB11 specifically controls the vesicle transport between the trans-Golgi network and the photoreceptive membrane. Moreover, PAB11 may possibly inform the condition of the photoreceptive membrane because the morphological phenotype of photoreceptor cells in this mutant closely resembles to that of the photoreceptor cells abnormally activated by light.4. The early step of endocytosis in the photoreceptor membrane region is controlled by RAB5. In contrast, the recycling of the synaptic vesicles, a major endocytic system in the axonal region, is independent of RAB5. This suggests that the axonal region would develop the partly self-supported system in the photoreceptor cell.

本课题以果蝇感光细胞为模型细胞，研究了囊泡运输的网络系统及其基本步骤之间的通讯，特别是Rab蛋白。为了这个目的，我调查的组织和胞内分布的Rab蛋白，并进行了生化和形态学分析的表型的显性负突变体，这些Rab蛋白。研究结果如下：1.定位于光感受膜结构域以及轴突结构域中的蛋白质在RAB 1.2的控制下从rER转运到高尔基体。RAB 2可能控制从高尔基体到光感受膜、轴突膜和溶酶体的所有通路。这表明RAB2将这些途径联系起来。RAB11特异性地控制囊泡在高尔基体网络和感光膜之间的运输。此外，PAB11可能告知感光膜的状况，因为该突变体中的感光细胞的形态表型与光异常激活的感光细胞的形态表型非常相似。在感光膜区域内吞作用的早期步骤由RAB 5控制。相比之下，突触囊泡（轴突区域的主要内吞系统）的回收不依赖于RAB 5。这表明轴突区在感光细胞中形成了部分的自我支持系统。