Novel function of pigment granules in the pigment cells of retina

视网膜色素细胞中色素颗粒的新功能

• 批准号: 17570059
• 负责人: OZAKI Koichi
• 金额: $ 2.3万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570059/
• 关键词: Pigment cell; Drosophila melanogaster; Retina; Retinoid; Fatty acid-binding protein; Retinol-binding protein; Photoreception; Vision; レチノール

Pigment cells of the Drosophila retina contain numerous pigment granules. We purified these granules and identified 2 proteins (fatty acid-binding protein (FBP) and photoreceptor/pigment cell dehydrogenase (PDH)) possibly involved in the visual cycle. To investigate the function of FBP and PDH, we first carried out immunohistochemical localization of these proteins. The results demonstrated that both proteins are localized on the surface of pigment granules as well as in the cytoplasm of retinal pigment cells. We next examined the binding activity of FBP to all-trans retinol with the use of the protein expressed in E. coli cells. The results demonstrated that retinol was specifically bound to FBP. Furthermore, we showed that FBP could be coimmunoprecipitated with PDH, suggesting that FBP and PDH would be involved in a common pathway of retinoid metabolism and directly interact with each other. We next modified the in vivo expression of FBP by overexpressing wild-type FBP or suppressing it with dsRNA. The HPLC analysis of retinoid composition in the mutant flies demonstrated that FBP is involved in the dark isomerization of 11-cis retinol to all-trans isomer. These results thus suggest that FBP and PDH are involved in the dark isomerization and oxidation of 11-cis retinol to all-trans retinal in the Drosophila retinal pigment cells, which may play a crucial role in the visual cycle like retinal pigment epithelium cells in the vertebrate eye.

果蝇视网膜的色素细胞含有大量的色素颗粒。我们纯化了这些颗粒，并确定了2个蛋白质（脂肪酸结合蛋白（FBP）和感光/色素细胞脱氢酶（PDH））可能参与视觉周期。为了研究FBP和PDH的功能，我们首先对这些蛋白进行了免疫组织化学定位。结果表明，这两种蛋白质定位于色素颗粒的表面以及在视网膜色素细胞的细胞质中。我们接下来使用在大肠杆菌中表达的蛋白质检测了FBP与全反式视黄醇的结合活性。coli细胞。结果表明，视黄醇与FBP特异性结合。此外，我们发现，FBP可以与PDH免疫共沉淀，这表明，FBP和PDH将参与一个共同的途径，类维生素A代谢，并直接相互作用。我们接下来通过过表达野生型FBP或用dsRNA抑制它来修饰FBP的体内表达。对突变果蝇中类维生素A组成的HPLC分析表明，FBP参与了11-顺式视黄醇向全反式异构体的暗异构化。因此，这些结果表明，在果蝇视网膜色素细胞中，FBP和PDH参与了11-顺式视黄醇的暗异构化和氧化为全反式视黄醇，这可能在视觉周期中起着至关重要的作用，如在脊椎动物眼睛的视网膜色素上皮细胞。

项目成果

Metabolic pathways of retinoid in the pigment cells of Drosophila retina.

果蝇视网膜色素细胞中类维生素A的代谢途径。

• 发表时间: 2006
• 期刊: Comparative Biochemistry and Physiology, Part B 145・3-4
• 作者: 山内兄人;新井康允(共編著);山内兄人;Koichi Ozaki;Koichi Ozaki
• 通讯作者: Koichi Ozaki

Rabll mediates post-Golgi trafficking of rhodopsin to the photosensitive apical membrane of Drosophila photoreceptors.

Rabll 介导高尔基体后视紫红质向果蝇光感受器的光敏顶膜的运输。

• 发表时间: 2005
• 期刊: Development 132・7
• 作者: K.Ozaki;S.Miyazono;N.Gombi;D.Ohno;Koichi Ozaki;Koichi Ozaki;Akiko K. Satoh
• 通讯作者: Akiko K. Satoh

Function of fatty acid-binding protein(FBP) in the Drosophila retina.

脂肪酸结合蛋白（FBP）在果蝇视网膜中的功能。

• 发表时间: 2006
• 期刊: Zoological Science 23・12
• 作者: K.Ozaki;S.Miyazono;N.Gombi;D.Ohno;Koichi Ozaki
• 通讯作者: Koichi Ozaki

Function of fatty acid-binding protein (FBP) in the Drosophila retina.

果蝇视网膜中脂肪酸结合蛋白（FBP）的功能。

• 发表时间: 2006
• 期刊: Zoological Science 23・12
• 作者: 山内兄人;新井康允(共編著);山内兄人;Koichi Ozaki
• 通讯作者: Koichi Ozaki

An odorant-binding protein facilitates odorant transfer from air to hydrophilic surroundings in the blowfly.

• DOI: 10.1093/chemse/bji049
• 发表时间: 2005-09
• 期刊: Chemical senses
• 影响因子: 3.5
• 作者: K. Tsuchihara;K. Fujikawa;M. Ishiguro;Takuji Yamada;C. Tada;K. Ozaki;M. Ozaki