Development of an artificial liposome to detect exosome markers in wound exudate

开发人工脂质体来检测伤口渗出液中的外泌体标记物

• 批准号: 22K18405
• 负责人: 真田 弘美
• 金额: $ 16.39万
• 依托单位: Ishikawa Prefectural Nursing University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Research (Pioneering)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-06-30 至 2026-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K18405/
• 关键词: 創傷アセスメント; 褥瘡; 滲出液; エクソソーム; miRNA

治癒に数か月以上を要する褥瘡の治癒期間を短縮するために、本研究では創部に貼付した創傷被覆材が創の上で自動的に創部アセスメントを行う『on woundアセスメント』を提唱し、滲出液エクソソームとの融合能を有する人工リポソームを固相化した創傷被覆材を開発する。人工リポソームにはmiRNAを体表温度を利用して特異的に増幅する低温反応性LAMP反応液を充填する。本目的を達成するために、（1）褥瘡滲出液のエクソソームおよびエクソソームmiRNAの分析、(2)人工リポソームを使った滲出液エクソソームmiRNA検出技術の確立、(3)人工リポソームを固相化した創傷被覆材の作製、および(4)滲出液miRNA検出能を備えた創傷被覆材の妥当性および効果検証を、臨床調査、In vitro実験、動物実験を組み合わせて実施する。本年度は、本課題の基盤技術となるリポソーム作製法について研究協力者である竹内昌治先生より技術移転を受け、本学内に新たな設備を準備し、再現性の高いリポソーム作製プロトコルを完成させた。また、検出技術として低温反応性LAMP法の確立のため、従来のLAMP法の実施と、低温反応性プローブの作製を試みた。

For more than a few months, it is necessary to treat the disease for more than a few months. in this study, the department of this study is responsible for the automatic application of the coated material in the system, which is called "on wound production system", and the production system can be used to improve the performance of the laminated material. The body surface temperature of miRNA is filled with low temperature anti-temperature LAMP solution. The purpose of this paper is: (1) to make sure that the technology is correct, (1) to make sure that the liquid is used, (1) to make sure that the liquid is produced, (1) to make sure that the liquid is produced, (1) to prepare the liquid, (1) to analyze the miRNA, (2) to make the liquid out of the machine, to make sure that the technology is correct, (3) to make sure that the technology is correct, (3) to use artificial equipment to solidify the coating material, The effluent miRNA can be used to determine the appropriateness of the clad material, such as the temperature, the bed temperature, the In vitro temperature, and the mechanical properties. This year, this year, this year's basic technical training program, this year's basic technical training The low-temperature anti-thermal LAMP method is used to determine the temperature response, the LAMP method is applied, and the low-temperature anti-thermal test is used.

项目成果

Development of mRNA markers of wound infection in fresh exudate of rat rull-thickness wounds.

大鼠全厚伤口新鲜渗出液中伤口感染 mRNA 标记的发展。

• 发表时间: 2022
• 作者: Takizawa C;Minematsu T;Nakagami G;Kitamura A;Koudounas S;Kunimitsu M;Sanada H.
• 通讯作者: Sanada H.

Expression levels of NPPB, ITGB6, CPNE4, EML5, and ITSN1 in fresh exudates swabbed from critically colonised and infected full-thickness wounds in rats.

NPPB，ITGB6，CPNE4，EML5和ITSN1的表达水平在大鼠的批判性殖民和感染的全厚性伤口中擦拭。

• DOI: 10.1111/iwj.13965
• 发表时间: 2023-04
• 期刊: INTERNATIONAL WOUND JOURNAL
• 影响因子: 3.1
• 作者: Takizawa, Chihiro;Minematsu, Takeo;Nakagami, Gojiro;Kitamura, Aya;Koudounas, Sofoklis;Kunimitsu, Mao;Sanada, Hiromi
• 通讯作者: Sanada, Hiromi

石川県立看護大学

石川县立看护专门学校