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A STUDY OF GENES DIFFERENTIALLY EXPRESSED BY OSTEOCLASTOGENIC MOUSE STROMAL CELLS

破骨细胞小鼠基质细胞差异表达基因的研究

基本信息

批准号：
09557021
负责人：
KITAZAWA Riko
金额：
$ 5.63万
依托单位：
KOBE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557021/
关键词：
postmenopausal osteoporosis osteoclast stromal cell RANKL TRANCE ODF OPGL promoter in situ hybridizatin cDNAクローニング

项目摘要

Osteoclasts are multinucleated cells derived from hemopoietic cells. Osteoblastic/ stromal cell lineage contributes to the terminal differentiation of osteoclast precursor s through the direct cell-to-cell interaction mechanism (Takahashi et al., 1988). ST2 cells, mouse stromal cell line, support osteoclastogenesis in the coculture with hematopoietic cells in the presence of Vitamin D3 and Dexamethasone. We found the phenomenon of the passage-dependent reduction of osteoclastogenesis in the co-culture system of ST2 and hemopoietic cells. In this study we attempted to isolate the molecules of which gene expression is associated to the passage-dependent capability of supporting osteoclastogenesis, and got 1 .5 kb cDNA of that molecule.Recently osteoclast differentiation factor (RANKL/ TRANCE/ OPGL/ ODF) was identified by others (H.Yasuda et al., P.N.A.S., 1998 ; D.Lacy et al., Cell, 1998). We then cloned and analyzed of 5-flanking basic promoter region of the mouse RANKL gene and attempted to elucidate the regulatory mechanism of RANKL gene expression in relation to the passage-dependent capability of ST2 cells to support osteoclastogenesis. To assess the expression of genes involved in osteoclastogenesis on the bone tissues, we set up the system of in situ hybridization using decalcified bone specimen. We have presented our data at various international as well as domestic meetings, and publish a lot of scientific papers for academic journals.

破骨细胞是源自造血细胞的多核细胞。成骨细胞/基质细胞谱系通过直接的细胞间相互作用机制促进破骨细胞前体的终末分化（Takahashi et al., 1988）。 ST2 细胞（小鼠基质细胞系）在维生素 D3 和地塞米松存在下支持与造血细胞共培养的破骨细胞生成。我们在ST2和造血细胞的共培养体系中发现了破骨细胞生成的传代依赖性减少的现象。在本研究中，我们试图分离其基因表达与支持破骨细胞生成的传代依赖性能力相关的分子，并获得该分子的 1 .5 kb cDNA。最近破骨细胞分化因子（RANKL/ TRANCE/ OPGL/ ODF）被其他人鉴定（H.Yasuda 等人，P.N.A.S.，1998；D.Lacy 等人，Cell， 1998）。然后，我们克隆并分析了小鼠 RANKL 基因的 5 侧翼基本启动子区域，并试图阐明 RANKL 基因表达与 ST2 细胞支持破骨细胞生成的传代依赖性能力相关的调节机制。为了评估骨组织中破骨细胞生成相关基因的表达，我们使用脱钙骨标本建立了原位杂交系统。我们在各种国际和国内会议上展示了我们的数据，并在学术期刊上发表了大量科学论文。