Formation of soft by transplanting and maturing preadipocytes

结缔组织具有潜在的自我更新和重塑能力，以应对损伤。在这项研究中，我们首次展示了结缔组织的这一特性可以用于整形和重建手术，以在理想的位置重建所需体积的丢失的软组织。我们的结果可以概括为以下几点。Kawaguchi et al.报道(细胞技术，26,354-361,1998)将培养的3T3-F442a细胞(前脂肪细胞)皮下注射到裸鼠体内，4周后导致脂肪垫形成。组织切片可见发达的脂肪细胞与血细胞一起出现。Toriyama等人。组织培养工程，24,143-146,1998)证实，当包裹有培养的前脂肪细胞的微珠注射到小鼠胸腔时，间充质细胞迁移并用胶囊包裹注射塞子。在含有bFGFs的珠子周围有分化为成熟脂肪细胞的现象，而没有bFGFF的珠子周围没有分化成成熟脂肪细胞。报道了小鼠在注射基底膜和碱性成纤维细胞生长因子部位的新生脂肪生成。S，95,1062-1066,1998)重组基底膜(Matrigel)加碱性成纤维细胞生长因子注射到裸鼠体内的结果。在1周内诱导新生血管后，内源性脂肪前体细胞迁移，2周内形成清晰可见的脂肪垫。这种新生脂肪形成在任何位置都可以被诱导。因此，我们的结果指出了一种在整形和重建外科中修复丢失软组织的新技术。

Connective tissue has the potential ability to renew and remodel itself in response to injury. In this research, we showed for the first time that this characteristic of connective tissue could be used in plastic and reconstructive surgery for reconstruction of a desired volume of lost soft tissue at a desired position. Our results can be summarized as follows. Kawaguchi et al. Reported (Cytotechnology, 26, 354-361,1998) that subcutaneous injection of cultured 3T3-F442A cells (pre-adipocyte) into nude mice resulted in fat pad formation after 4 weeks. Histological section of the pad showed well-developed adipocytes appearing with blood cells. Toriyama et al. demonstrated (Tissue Culture Engineering, 24, 143-146, 1998) that mesenchymal cells migrated and wrapped the injected plug with the capsule when microbeads coated with cultured pre-adipocytes were injected into mice chests. Differentiation into mature adipose cells was found around the beads with bFGF while no differentiation was found around the beads without bFGF.Kawaguchi et al. reported (De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor. Proc Natl Acad Sci U S A.95, 1062-1066, 1998) the results of injecting reconstituted basement membrane (Matrigel) supplemented with bFGF into nude mice. The neovasculization induced within 1 week was followed by migration of endogenous adipose precursor cells, and a clearly visible fat pad was formed within 2 weeks. Such de novo adipogenesis was induced at any position. Thus our results point to a new technique of augmentation of lost soft tissues in plastic and reconstructive surgery.