Formation of soft by transplanting and maturing preadipocytes

通过移植和成熟前脂肪细胞形成软组织

• 批准号: 09557122
• 负责人: TORII Shuhei
• 金额: $ 7.87万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557122/
• 关键词: mesenchymal stem cell; reconstitute basement membrane; adipose tissue; fibroblast growth factor; soft tissue; 間葉系細胞膜

Connective tissue has the potential ability to renew and remodel itself in response to injury. In this research, we showed for the first time that this characteristic of connective tissue could be used in plastic and reconstructive surgery for reconstruction of a desired volume of lost soft tissue at a desired position. Our results can be summarized as follows. Kawaguchi et al. Reported (Cytotechnology, 26, 354-361,1998) that subcutaneous injection of cultured 3T3-F442A cells (pre-adipocyte) into nude mice resulted in fat pad formation after 4 weeks. Histological section of the pad showed well-developed adipocytes appearing with blood cells. Toriyama et al. demonstrated (Tissue Culture Engineering, 24, 143-146, 1998) that mesenchymal cells migrated and wrapped the injected plug with the capsule when microbeads coated with cultured pre-adipocytes were injected into mice chests. Differentiation into mature adipose cells was found around the beads with bFGF while no differentiation was found around the beads without bFGF.Kawaguchi et al. reported (De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor. Proc Natl Acad Sci U S A.95, 1062-1066, 1998) the results of injecting reconstituted basement membrane (Matrigel) supplemented with bFGF into nude mice. The neovasculization induced within 1 week was followed by migration of endogenous adipose precursor cells, and a clearly visible fat pad was formed within 2 weeks. Such de novo adipogenesis was induced at any position. Thus our results point to a new technique of augmentation of lost soft tissues in plastic and reconstructive surgery.

结缔组织具有响应损伤而自我更新和重塑的潜在能力。在这项研究中，我们首次表明结缔组织的这种特性可用于整形和重建手术，以在所需位置重建所需体积的丢失软组织。我们的结果可以总结如下。川口等人报道（细胞技术，26，354- 361，1998），将培养的3 T3-F442 A细胞（前脂肪细胞）皮下注射到裸小鼠体内，4周后导致脂肪垫形成。垫的组织切片显示发育良好的脂肪细胞与血细胞一起出现。Toriyama等人证明（Tissue Culture Engineering，24，143-146，1998），当将包被有培养的前脂肪细胞的微珠注射到小鼠胸部时，间充质细胞迁移并用囊包裹注射的塞子。川口等人报道（De novo adipogenesis in mice at the site of injection of basic membrane and basic fibroblast growth factor. Proc Natl Acad Sci U S A. 95，1062-1066，1998）将补充有bFGF的重构基底膜（Matrigel）注射到裸鼠中的结果。在1周内诱导的新血管形成之后是内源性脂肪前体细胞的迁移，并且在2周内形成清晰可见的脂肪垫。这种从头脂肪形成在任何位置被诱导。因此，我们的研究结果指出了一种新的技术，增加失去的软组织的整形和重建手术。

项目成果

Nobuko Kawaguchi: "Reconstituted basement menbrane potentiates in vivo adipogenesis of 3T3-F442A cells" Cytotechnology. (印刷中). (1998)

Nobuko Kawaguchi：“重建的基底膜增强 3T3-F442A 细胞的体内脂肪生成”《细胞技术》（出版中）。

Nobuko Kawaguchi: "Reconstitued basement membrane potentiates in vivo adipogenesis of 3T3-F442A cells." Cytotechnology. (in press).

Nobuko Kawaguchi：“重建的基底膜增强了 3T3-F442A 细胞的体内脂肪生成。”

Nobuko Kawaguchi, Kazuhiro Toriyama, Eleni Nicodemou-Lena, Kazuhiko Inou, Shuhei Torii, and Yasuo Kitagawa: "De novo adipogenesis in mice at the site of injection of basement membrane and basic fibroblast growth factor." Proc.Natl.Acad.Sci.USA. 95. 1062-1

Nobuko Kawaguchi、Kazuhiro Toriyama、Eleni Nicodemou-Lena、Kazuhiko Inou、Shuhei Torii 和 Yasuo Kitakawa：“在注射基底膜和碱性成纤维细胞生长因子的部位，小鼠的脂肪从头生成。”

Nobuko Kawaguchi, Kazuhiro Toriyama, Eleni Nicodemou-Lena, Kazuhiko Inou, Shuhei Torii, and Yasuo Kitagawa: "Reconstituted basement membrane potentiates in vivo adipogenesis of 3T3-F442A" Cytotechnology. (in press).

Nobuko Kawaguchi、Kazuhiro Toriyama、Eleni Nicodemou-Lena、Kazuhiko Inou、Shuhei Torii 和 Yasuo Kitakawa：“重建基底膜增强 3T3-F442A 体内脂肪生成”细胞技术。

Nobuko Kawaguchi: "Reconstitued basement membrane potentiates in vivo adipogenesis of 3T3-F442A cells." Cytotechnology. in press.

Nobuko Kawaguchi：“重建的基底膜增强了 3T3-F442A 细胞的体内脂肪生成。”