Tissue reconstrudion with Ox vivo culturad auto logous mesenchymal stem cells

自体脂肪垫移植在修复丢失的软组织的整形和重建外科手术中有着悠久的历史。自体脂肪移植、珍珠和半液态脂肪组织在面部凹陷区或疤痕的治疗中的许多应用已有报道。尽管人们热衷于改进自体脂肪移植物的操作，但随着时间的推移，移植物的逐渐吸收让研究人员感到失望。脂肪母细胞作为单潜能脂肪细胞的前体细胞，其成脂作用在胚胎发育过程中完成已被公认。出生后不会有脂肪母细胞的供应。这就是为什么外科手术对丢失的脂肪组织的论证一直只集中在移植预先形成的脂肪垫上。然而，我们最近在基底膜注射部位对小鼠新生脂肪形成的观察表明，成体bo…结缔组织中分布着大量的脂肪前体细胞。它们可以迁移、增殖和成熟以形成：所需大小和所需位置的脂肪垫。由于这一结果暗示了一种新的扩大丢失的J软组织的技术，我们在这里探索通过分离和体外培养这些脂肪细胞前体细胞作为脂肪垫扩大的自体细胞的可能性。在Yamaguchi等人(2000年)中，我们分析了由脂肪细胞前体细胞和内皮细胞合成的层粘连蛋白α4链的G结构域的结构和功能，以构建这两种细胞之间的基底膜结构。在Toriyama和Kawaguchi(2001)中，我们回顾了我们通过注射Matrigel在小鼠体内进行新生脂肪生成的成功。在Tajima等人(2001)中，我们发现3T3-L1成纤维细胞在低氧条件下由于刺激IV型胶原合成而加速脂肪转化。在Goto等人(2001年)中，我们利用链特异性α，β.γ干扰分析了果蝇层粘连蛋白RNA链的细胞内组装过程。在Kitagawa和Yamashita(2001)中，我们介绍了我们关于层粘连蛋白A链G结构域在外周结缔组织中功能的最新数据。在Toriyama等人(2002)中，我们报道了再生软组织工程中脂肪细胞前体细胞的电子显微镜组织学。较少

Auto-grafting of fat pads has a long history in plastic and reconstructive surgery for augmentation of lost soft tissue. Many uses of autogenous fat grafts, pearls and semi liquid adipose tissue in the treatment of depressed regions or scars in the facial area have been reported. Despite the enthusiasm for improved operation of free fat-autografts, however, researchers have been disappointed by progressive absorption of the graft with time. It has been accepted that the commitment of adipoblasts, unipotential precursor of adipocytes, completes during the embryonic development. Postnatal supply of adipoblasts has not been Expected. This is the reason why the surgery for argumentation of lost fat tissue has been focused only on transplantation of pre-formed fat pads. Our recent observation of de novo adipogenesis in mice at the site of injection of basement membrane, however, suggested that an abundant population of adipose precursor cells is distributed in connective tissues of adult bo … More dy and that they can migrate, proliferate and maturate to form : fat pads with desired size and at desired site. Since this result implies a novel technique of augmenting lost j soft tissue, we here explore for the possibility of taking advantage of such adipocyte precursor cells by isolating and culturing them ex vivo as the autogenous cell for fat pad augmentation.In Yamaguchi et al, (2000), we have analyzed the structure and function of the G domain of laminin α4 chain which is synthesized by both adipocyte precursor cells and endothelial cells for the construction of the basemenf membrane structure between these tow types of cells. In Toriyama and Kawaguchi (2001), we reviewed our success of de novo adipogenesis in mice by injection of Matrigel. In Tajima et al, (2001), we found accelerated adipose conversion of 3T3-L1 fibroblasts under hypoxia due to stimulated type IV collagen synthesis. In Goto et al, (2001), we analyzed intracellular process of the assembly of Drosophila laminin α, β.γ Chains taking advantage of chain-specific RNA interference. In Kitagawa and Yamashita (2001), we introduced our recent data on the function of laminin a chain G domain in the peripheral connective tissue. In Toriyama et al, (2002), we reported an electron-microscopic histology of the adipocyte precursor cells for regenerative soft-tissue engineering. Less