Tissue reconstrudion with Ox vivo culturad auto logous mesenchymal stem cells

自体脂肪垫移植在整形和重建手术中有着悠久的历史，用于增加失去的软组织。自体脂肪移植物、珍珠和半液体脂肪组织在治疗面部凹陷区域或疤痕方面的许多应用已被报道。尽管人们对自体游离脂肪移植手术的改进充满热情，但随着时间的推移，移植物的逐渐吸收使研究人员感到失望。脂肪细胞是脂肪细胞的单电位前体，在胚胎发育过程中完成了对脂肪细胞的承诺。没有预料到产后脂肪细胞的供应。这就是为什么脂肪组织切除手术一直只集中在预先形成的脂肪垫的移植上。然而，我们最近对小鼠基底膜注射部位的新生脂肪形成的观察表明，大量的脂肪前体细胞分布在成年bo…More dy的结缔组织中，它们可以迁移、增殖和成熟，形成所需大小和所需位置的脂肪垫。由于这一结果意味着一种增加丢失的软组织的新技术，我们在这里探索通过分离和体外培养这些脂肪细胞作为脂肪垫增加的自体细胞来利用这些脂肪细胞前体细胞的可能性。Yamaguchi et al .（2000）分析了脂肪前体细胞和内皮细胞合成的层粘连蛋白α4链G结构域的结构和功能，构建了这两种细胞之间的基本膜结构。在Toriyama和Kawaguchi（2001）中，我们回顾了通过注射Matrigel在小鼠体内成功的新生脂肪形成。在Tajima等人（2001）的研究中，我们发现缺氧条件下3T3-L1成纤维细胞的脂肪转化由于刺激了IV型胶原合成而加速。在Goto等人（2001）中，我们分析了果蝇层粘连蛋白α， β的细胞内组装过程。利用链特异性RNA干扰的γ链。在Kitagawa和Yamashita（2001）中，我们介绍了我们关于层粘连蛋白a链G结构域在外周结缔组织中的功能的最新数据。在Toriyama et al，（2002）中，我们报道了用于再生软组织工程的脂肪前体细胞的电镜组织学。少

Auto-grafting of fat pads has a long history in plastic and reconstructive surgery for augmentation of lost soft tissue. Many uses of autogenous fat grafts, pearls and semi liquid adipose tissue in the treatment of depressed regions or scars in the facial area have been reported. Despite the enthusiasm for improved operation of free fat-autografts, however, researchers have been disappointed by progressive absorption of the graft with time. It has been accepted that the commitment of adipoblasts, unipotential precursor of adipocytes, completes during the embryonic development. Postnatal supply of adipoblasts has not been Expected. This is the reason why the surgery for argumentation of lost fat tissue has been focused only on transplantation of pre-formed fat pads. Our recent observation of de novo adipogenesis in mice at the site of injection of basement membrane, however, suggested that an abundant population of adipose precursor cells is distributed in connective tissues of adult bo … More dy and that they can migrate, proliferate and maturate to form : fat pads with desired size and at desired site. Since this result implies a novel technique of augmenting lost j soft tissue, we here explore for the possibility of taking advantage of such adipocyte precursor cells by isolating and culturing them ex vivo as the autogenous cell for fat pad augmentation.In Yamaguchi et al, (2000), we have analyzed the structure and function of the G domain of laminin α4 chain which is synthesized by both adipocyte precursor cells and endothelial cells for the construction of the basemenf membrane structure between these tow types of cells. In Toriyama and Kawaguchi (2001), we reviewed our success of de novo adipogenesis in mice by injection of Matrigel. In Tajima et al, (2001), we found accelerated adipose conversion of 3T3-L1 fibroblasts under hypoxia due to stimulated type IV collagen synthesis. In Goto et al, (2001), we analyzed intracellular process of the assembly of Drosophila laminin α, β.γ Chains taking advantage of chain-specific RNA interference. In Kitagawa and Yamashita (2001), we introduced our recent data on the function of laminin a chain G domain in the peripheral connective tissue. In Toriyama et al, (2002), we reported an electron-microscopic histology of the adipocyte precursor cells for regenerative soft-tissue engineering. Less