Development of systematic approach in determining three-dimensional structure of membrane bound biomolecules based on accurate interatomic distances

在这一研究项目中，我们研究了一种基于膜束生物分子的三维结构来确定从固态核磁共振中获得的精确互原子距离的系统方法。在这一研究项目中取得的结果。（1） The three-dimensional structure of [イイD113イエD1C，イイD115イエD1N]-labeled Leu-enkephalin （tyr-Gly-Gly-Phe-Leu） dihydrate crystals was determined on the basis of six sets of accurately determinedイD11C-イD115イD1N interatomic distances by rotational double resonance （REDOR） and some additional constraints from echo D11C chemical shifts。（2） The phenyl ring dynamics of [イイD12イイD1HイD25イD2]PheイD14イD1-labeled LeuイD15イD1-and MetイD15イD1-enkephalin molecules in crystals grown from four solvents were examined using solid stateイD12イD1H NMR spectroscopy。您的位置：知道173>> D1 H NMR？pattern clearly indicated the presence of 180°flip motions about the C？bond axis of the phenyl rings。的差异性。 ... More 这种运动的频率被归因于他们在水晶中的分子包装的使命。（3） Conformational transition of human calcitonin （hCT） during fibril formation in the acidic and neutral condition were investigated by high resolution solid state-D113-D1C NMR spectroscopy。结果表明，从α-螺旋到β-表的规范性过渡，以及从随机线圈到β-表形式在中央和C-终端区域发生的过程中，在光纤形成期间发生。（4） The conformation and dynamics of melittin bound to the dimyristoylphophatidylcholin （DMPC） bilayer and the magnetic orientation in the lipid bilayer systems were investigated by solid-stateイD131イエD1P andイイD113イエD1C NMR spectroscopy。使用D131-D1 P核磁共振，发现了一种以液态水晶球凝胶相转换温度为平行的长轴延伸的载体，该载体以磁性方向延伸到液态水晶球凝胶相转换温度。伊D113伊D1C NMR spectra were observed on the伊D113伊D1C labeled melittin bound to the lipid bilayer。最后，我们发现了一个melittin采用一个跨膜α-螺旋，其中平均轴与Billayers正常平行。Less（低）

In this research project, we have studied to establish systematic approach in determining the three-dimensional structure of membrane bound biomolecules based on accurate interatomic distances obtained from solid state NMR. Following results were obtained in this research project.(1) The three-dimensional structure of [ィイD113ィエD1C, ィイD115ィエD1N]-labeled Leu-enkephalin (tyr-Gly-Gly-Phe-Leu) dihydrate crystals was determined on the basis of six sets of accurately determined ィイD113ィエD1C-ィイD115ィエD1N interatomic distances by rotational echo double resonance (REDOR) and some additional constraints from ィイD113ィエD1C chemical shifts.(2) The phenyl ring dynamics of [ィイD12ィエD1HィイD25ィエD2]PheィイD14ィエD1-labeled LeuィイD15ィエD1- and MetィイD15ィエD1-enkephalin molecules in crystals grown from four solvents were examined using solid state ィイD12ィエD1H NMR spectroscopy. ィイD12ィエD1H NMR powder pattern clearly indicated the presence of 180°flip motions about the Cβ-Cγbond axis of the phenyl rings. The difference of … More the frequencies for the motion was attributed to the manner of their molecular packing in the crystal.(3) Conformational transition of human calcitonin (hCT) during fibril formation in the acidic and neutral condition were investigated by high resolution solid state ィイD113ィエD1C NMR spectroscopy. The results indicate that conformational transitions from α-helix to β-sheet, and from random coil to β-sheet forms occurred in the central and C-terminus regions, respectively, during fibril formation.(4) The conformation and dynamics of melittin bound to the dimyristoylphophatidylcholin (DMPC) bilayer and the magnetic orientation in the lipid bilayer systems were investigated by solid-state ィイD131ィエD1P and ィイD113ィエD1C NMR spectroscopy. Using ィイD131ィエD1P NMR, it was found that melittin-DMPC bilayer system forms magnetically oriented elongated vesicles with the long axis parallel to the magnetic field above the liquid crystalline-gel phase transition temperature. ィイD113ィエD1C NMR spectra were observed on the ィイD113ィエD1C labeled melittin bound to the lipid bilayer. Finally, it was found that melittin adopts a transmembrane α-helix whose average axis is parallel to the bilayers normal. Less