The application of "green mouse" as a versatile experimental animal.

“绿色小鼠”作为多功能实验动物的应用。

• 批准号: 09558106
• 负责人: OKABE Masaru
• 金额: $ 6.53万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09558106/
• 关键词: GFP; trangenic mouse; X chromosome; Y chromosome; X inactivation; 凍結保存

The green fluorescent protein (GFP) is responsible for the green bioluminescence of the jellyfish Aequorea victoria. Many classes of GFP mutants exist that display modified fluorescence spectra and an increased extinction coefficient. We produced transgenic mouse lines with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments. We have succeeded to safely frozen this precious transgenic mouse lines and have proliferated to distributed to the scientific world. Up to now, more than 100 laboratories have received the green mice".In order to separate the X and Y baring embryo, we have produced many lines of transgenic mouse. Among then, we had succeeded to produce three lines of X-linked GFP and one line of Y-linked GFP.These mice would serve as a study in separation of male and female embryos before implantation. It would also serve as a powerful tool to investigate the mechanism of X inactivation.

绿色荧光蛋白(GFP)负责水母的绿色生物发光。存在许多类型的GFP突变体，它们表现出修改的荧光光谱和增加的消光系数。在鸡β-肌动蛋白启动子和巨细胞病毒增强子的控制下，我们获得了含有“增强型”绿色荧光蛋白(EGFP)基因的转基因小鼠。这些转基因小鼠品系的细胞的荧光性质将有助于它们在许多类型的细胞移植实验中使用。我们已经成功地安全地冷冻了这些珍贵的转基因小鼠品系，并已增殖并分发给科学界。到目前为止，已有100多个实验室收到了绿色小鼠。为了分离X和Y胚胎，我们培育了多个转基因小鼠系。其中，我们成功地获得了3个X-连锁GFP系和1个Y-连锁GFP系。这些小鼠将作为着床前分离雌雄胚胎的研究工具，也将为研究X失活的机制提供有力的工具。

项目成果

M.Ikawa, S.Yamada, T.Nakanishi and M.Okabe: "'Green mice' and their potential usage in biological research." FEBS Lett. 430. 83-7 (1998)

M.Ikawa、S.Yamada、T.Nakanishi 和 M.Okabe：“‘绿色小鼠’及其在生物研究中的潜在用途。”

Okabe, M.et al.: "Green fluorescent protein and its usage in reproductive biology" Zygote. 6. 117 (1998)

Okabe, M.et al.：“绿色荧光蛋白及其在生殖生物学中的用途”Zygote。

M.Okabe, M.Ikawa, K.Kominami, T.Nakanishi and Y.Nishimune: "'Green mice' as a source of ubiquitous green cells." FEBS Lett. 407. 313-9 (1997)

M.Okabe、M.Ikawa、K.Kominami、T.Nakanishi 和 Y.Nishimune：“‘绿色小鼠’是无处不在的绿色细胞的来源。”

Okabe,M.et al.: "'Green mice'as a source of ubiquitous green cells" FEBS Lett. 407. 313-319 (1997)

Okabe,M.et al.：“‘绿色小鼠’是无处不在的绿色细胞的来源”FEBS Lett。

Hadjantonakis, A.K.et al.: "Non-invasive sexing of preimplantation stage mammalian embryos." Nat Genet. 19. 220-222 (1998)

Hadjantonakis, A.K.等人：“植入前阶段哺乳动物胚胎的非侵入性性别鉴定。”