Selection of male and female embryos before implantation

绿色荧光蛋白(GFP)负责水母的绿色生物发光。存在许多类型的GFP突变体，它们表现出修改的荧光光谱和增加的消光系数。在鸡β-肌动蛋白启动子和巨细胞病毒增强子的控制下，我们获得了含有“增强型”绿色荧光蛋白(EGFP)基因的转基因小鼠。除红细胞和毛发外，这些转基因品系的所有组织在激发光下都是绿色的。这些转基因小鼠品系的细胞的荧光性质为它们在多种细胞移植实验中的应用提供了便利。为了分离X和Y胚胎，我们培育了许多转基因小鼠品系。其中，我们成功地获得了3个X连锁的GFP和1个Y连锁的GFP，这些小鼠将作为植入前分离雄性和雌性胚胎的研究。它也将成为研究X失活机制的有力工具。

The green fluorescent protein (GFP) is responsible for the green bioluminescence of the jellyfish Aequorea victoria. Many classes of GFP mutants exist that display modified fluorescence spectra and an increased extinction coefficient. We produced transgenic mouse lines with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. All of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light. The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments.In order to separate the X and Y baring embryo, we have produced many lines of transgenic mouse. Among then, we had succeeded to produce three lines of X-linked GFP and one line of Y-linked GFP.These mice would serve as a study in separation of male and female embryos before implantation. It would also serve as a powerful tool to investigate the mechanism of X inactivation.