Selection of male and female embryos before implantation

植入前选择雄性和雌性胚胎

• 批准号: 08458238
• 负责人: OKABE Masaru
• 金额: --
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458238/
• 关键词: GFP; transgenic mouse; X chromosome; Y chromosome; X inactivation; green fluorescent protein; transgenic mouse; embryonic stem cell

The green fluorescent protein (GFP) is responsible for the green bioluminescence of the jellyfish Aequorea victoria. Many classes of GFP mutants exist that display modified fluorescence spectra and an increased extinction coefficient. We produced transgenic mouse lines with an "enhanced" GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. All of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light. The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments.In order to separate the X and Y baring embryo, we have produced many lines of transgenic mouse. Among then, we had succeeded to produce three lines of X-linked GFP and one line of Y-linked GFP.These mice would serve as a study in separation of male and female embryos before implantation. It would also serve as a powerful tool to investigate the mechanism of X inactivation.

绿色荧光蛋白（GFP）负责维多利亚水母的绿色生物发光。存在许多种类的GFP突变体，其显示改变的荧光光谱和增加的消光系数。我们生产了转基因小鼠品系与“增强”的绿色荧光蛋白（绿色荧光蛋白）的鸡β-肌动蛋白启动子和巨细胞病毒增强子的控制下的cDNA。除红细胞和毛发外，这些转基因品系的所有组织在激发光下均为绿色。这些转基因小鼠细胞的荧光特性将有助于它们在各种细胞移植实验中的应用。为了分离X和Y裸胚，我们已经生产了许多转基因小鼠品系。其中，我们成功地制备了三个X连锁的GFP和一个Y连锁的GFP小鼠，这些小鼠将作为植入前雌雄胚胎分离的研究。它也将成为研究X失活机制的有力工具。

项目成果

Ikawa, M., and Okabe, M.: "Green flourescent protein (GFP) as a marker in transgenic mice." J.Reprod.Dev.43. 181-2 (1997)

Ikawa, M. 和 Okabe, M.：“绿色荧光蛋白 (GFP) 作为转基因小鼠的标记。”

伊川 正人, 岡部 勝: "光るマウス!?" 化学と生物. 36. 140-141 (1998)

井川正人，冈部胜：“发光的老鼠！？” 36. 140-141 (1998)

Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T., and Nishimura, Y.: "'Green mice'as a source of ubiquitous green cells." FEBS Lett. 407. 313-9 (1997)

Okabe, M.、Ikawa, M.、Kominami, K.、Nakanishi, T. 和 Nishimura, Y.：“‘绿色小鼠’是无处不在的绿色细胞的来源。”

M.Okabe, et al.,: "'Green mice'as a source of ubiquitous green cells" FEBS Lett. 407,. 313-319 (1997)

M.Okabe 等人：“‘绿色小鼠’是无处不在的绿色细胞的来源”FEBS Lett。

伊川 正人, 岡部 勝: "GFPに関する情報" 細胞工学. 16. 642-643 (1997)

Masato Ikawa、Masaru Okabe：“有关 GFP 的信息”细胞工程 16. 642-643 (1997)。