Analysis using optical recording for synaptic plasticity of non-LTP type in hippocampal slices

海马切片中非 LTP 型突触可塑性的光学记录分析

• 批准号: 09480227
• 负责人: SEKINO Yuko
• 金额: $ 2.94万
• 依托单位: Gunma University, School of Medidcine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480227/
• 关键词: Hippocampus; Optical Recording; Adenosine; LTP; CA2; CA3a; 長期増強; 光学的測定; 膜電位感受性色素; 海馬スライス; 光学的測定法; アデノシンA1受容体

Using optical and field potential recordings, we have shown that there were two distinguished patterns of signal propagation, CA3-CA1 and CA3-CA2-CA1, in oblique slices obtained from rat hippocampus ; the, and the slow propagation. In the fast propagation, activity flowed directly from CA3 to the stratum radiatum of CA1, and in the slow propagation optical signals were generated in CA2 before spreading to CA1. Our data reveal that CA2 neurons can transmit delayed excitatory signals to CA1 neurons. We therefore conclude that consideration of electrical signal propagation through the hippocampus should include flow through the CA2 region in addition to the traditional dentate gyrus-CA3-CA1 trisynaptic circuit. When slices were treated with an adenosine antagonist (2 μM 8-CPT), population spikes (PSs) were recorded in the stratum pyramidale of CA1 and CA2 in almost all of transverse slices, where no PS was recorded without 8-CPT in most cases. This data indicated that there is strong puri … More nergic inhibition in CA2/CA3a and suggest that CA2 cells act as a gate of signal propagation in the transverse plane of hippocampus.We further described the immunocytochemical distribution of adenosine A1 receptors in the rat hippocampus. Adenosine A1 receptor-like immunoreactivity was seen on the cell soma and dendrites of pyramidal cells and the cell soma and proximal part of dendrites of granule cells, but not on glial cells. In the adult rat hippocampus, rostrocaudal inspection revealed that immunoreactivity in CA2/CA3a was greatest. Confocal microscopy revealed differences in the staining patterns for the adenosine A1 receptor and synaptophysin, a marker of presynaptic terminals. This suggests that the adenosine A1 receptor might have postsynaptic physiological functions. Double-labeling of adenosine A1 receptors and anterogradely labeled fibers from the supramammillary nucleus showed that the fibers from the supramammillary nucleus terminate directly on the cell soma of the A1 receptor immunopositive neurons in CA2/CA3a and the dentate gyrus.These results indicate that the adenosine A1 receptor in CA2/CA3a and the dentate gyrus are in a position to regulate hippocampal theta activity and that resultant strong synaptic depression in CA2/CA3a could play a role in regulating the intrinsic signal flow between CA3 and CA1. Less

使用光学和场电位记录，我们已经表明，有两个不同的模式的信号传播，CA 3-CA 1和CA 3-CA 2-CA 1，在斜切片从大鼠海马;的，和缓慢的传播。在快速传播中，活动直接从CA 3流到CA 1的辐射层，而在慢传播中，光信号在传播到CA 1之前在CA 2中产生。我们的数据表明，CA 2神经元可以传递延迟兴奋信号给CA 1神经元。因此，我们的结论是，考虑通过海马体的电信号传播应包括通过CA 2区除了传统的齿状回CA 3-CA 1三突触电路的流量。用腺苷拮抗剂（2 μM 8-CPT）处理脑片后，几乎所有横切面的CA 1和CA 2的齿状回层均记录到群体峰电位（PS），而在无8-CPT处理的情况下，大多数横切面的CA 1和CA 2的齿状回层未记录到PS。该数据表明存在较强的纯度 ...更多信息 结果表明，腺苷A_1受体在大鼠海马中的分布与腺苷A_1受体在海马中的分布有关，腺苷A_1受体在海马中的分布与腺苷A_1受体在海马中的分布有关。腺苷A1受体阳性反应见于锥体细胞的胞体索马和树突，颗粒细胞的胞体索马和树突近端，而胶质细胞上未见。在成年大鼠海马，喙尾检查显示，在CA 2/CA 3a的免疫反应性最大。共聚焦显微镜显示腺苷A1受体和突触素（突触前末梢的标记物）的染色模式存在差异。提示腺苷A1受体可能具有突触后生理功能。腺苷A_1受体和乳头上核顺行标记纤维的双标记表明，乳头上核的纤维直接终止于CA_2/CA_3a和齿状回的腺苷A_1受体免疫阳性神经元的细胞索马体上，提示CA_2/CA_3a的腺苷A_1受体与海马的腺苷A_1受体有关。CA 3a和齿状回处于调节海马theta活动的位置，并且由此产生的CA 2/CA 3a中的强突触抑制可以在调节CA 3和CA 1之间的内在信号流中发挥作用。少

项目成果

Y. Matsuoka, M. Okazaki, K. Tanaka, Y. Katamura, S. Ohta, Y. Sekino, T. Taniguchi: "Endogenous Adenosine Protects CA1 Neurons from Kainic Acid-Induced Neuronal Cell Loss in the Rat Hippocampus"Eur. J. Neurosci.. 11. 3617-3625 (1999)

Y. Matsuoka、M. Okazaki、K. Tanaka、Y. Katamura、S. Ohta、Y. Sekino、T. Taniguchi：“内源性腺苷保护 CA1 神经元免受红藻氨酸诱导的大鼠海马神经元细胞损失”Eur。

関野祐子(分担): "シリーズ・ニューバイオフィジックス(5)イオン チャネル" 日本生物物理学会シリーズ・ニューバイオフィジックス刊行委員会, 224 (1997)

Yuko Sekino（撰稿人）：《系列新生物物理学（5）离子通道》日本生物物理学会系列新生物物理学出版委员会，224（1997）

TOMOYO OCHIISHI,LING CHEN,AYA YUKAWA,YOSHIKO SAITOH,YUKO SEKINO,TAKAO ARAI,HIROYASU NAKATA,HIROSHI MIYAMOTO: "Cellular Localization of Adenoshine Al Receptors in Rat Forebrain : Immunohisto chemical Analysis Using Adenosine AI Receptor-Specific Monoclonal

TOMOYO OCHIISHI、Ling Chen、AYA YUKAWA、YOSHIKO SAITOH、YUKO SEKINO、TAKAO ARAI、HIROYASU NAKATA、HIROSHI MIYAMOTO：“大鼠前脑腺苷 Al 受体的细胞定位：使用腺苷 AI 受体特异性单克隆抗体进行免疫组织化学分析

Y. Sekino, K. Obata, M. Tanifuji, M. Mizuno and J. Murayama: "Delayed Signal Propagation via CA2 in Rat Hippocampal Slices Revealed by Optical Recording. 6."J. Neurophysiol.. 78. 1662-1668 (1997)

Y. Sekino、K. Obata、M. Tanifuji、M. Mizuno 和 J. Murayama：“光学记录揭示了大鼠海马切片中 CA2 的延迟信号传播。6”。

Ochiishi.T.et al Sekino,Y(CA): "High Level of Adenosine A_1 Receptor-like Immunoreactivity in the CA2/CA3 a Region at the Adult Rat Hippocimpus." Neuroscience. (in press). (1999)

Ochiishi.T.et al Sekino,Y(CA)：“成年大鼠海马 CA2/CA3 a 区域中腺苷 A_1 受体样免疫反应性水平很高。”