Identification and analysis of the genes that regurate the bahavior of the honeybee

调节蜜蜂行为的基因的鉴定和分析

• 批准号: 09480228
• 负责人: KUBO Takeo
• 金额: $ 8.51万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480228/
• 关键词: Insect; Social insect; Honeybee; Behavior; memory & learning; Mushroom body; cDNA; Calcium; 記憶; Ca^<2+>; IP3受容体; cDNAクローニング; RNA; 膜翅目

Honeybee is a social insect and many exquisite communications are performed among the colony members such as "dance launguage". However, the molecular basis that underlies such highly advanced types of honeybee behavior is largely unknown. The mushroom bodies (MB), which are supposed to be a main center of the senssory integration and memory, are characteristically well-developed in comparison with those of other insects. Thus, the increased complexity of the function and structure of the MB might be due to the highly advanced types of honeybee bahavior. Here, we searched for genes that are expressed preferentially in the MB by the differential display method as candidate genes that regulate the honeybee behavior, and obtained the following results.1. The intrinsic ceuron cells that consist the MB are subdivided into two classes : the large-and small-type Kenyon cells. A gene which is expressed specifically in the large-type Kenyon cells, termed M5, and a gene which is expressed specif … More ically in the small-type Kenyon cells, termed Ks, are identified for the first time. cDNAs for these genes did not contain significant long open reading frames, suggesting that the M5 and Ks gene transcripts function as non-translated RNAs. In situ hybridization analysis of the M5 gene transcripts in the pupal brain revealed that the honeybee MB is a quadruple structure consisting of four clonal units, each of wich contains two distinct areas that express different genes. Furthure analysis of the functions of these gene products will reveal the subdivision of the inner structure of the MB that uinderlie the honeybee behavior.2. We showed that the expression of the genes for proteins involved in the intracellular calcium signal transduction was up-regulated in the MB of the honeybee. In contrast, in Drosophila, these genes were reported to be expressed ubiquitously thoroughout the brain cortex. Calcium signal transduction system of the neural cell has been reported to be involved in the estabrishment of memory and learning. Thus, it seems possible that the enhancement of the function of the calcium signaling contributes to the enhanced sinaptic plasticity of the MB of the honeybee. These findinG will be inportant from the perspective of comparative neurobiology of the insect. Less

蜜蜂是一种群居昆虫，在蜂群成员之间进行着许多精致的交流，如舞蹈发射。然而，这种高度先进的蜜蜂行为背后的分子基础在很大程度上是未知的。蘑菇体(MB)被认为是感觉整合和记忆的主要中心，与其他昆虫相比，它的特征是发育得很好。因此，MB功能和结构的复杂性增加可能是由于蜜蜂行为的高度先进类型。在这里，我们通过差异显示的方法寻找在MB中优先表达的基因作为调控蜜蜂行为的候选基因，并获得了以下结果。组成MB的固有神经元细胞被细分为两类：大的和小型的Kenyon细胞。在大型凯尼恩细胞中特异性表达的基因，称为M5，以及特异性表达…的基因更确切地说，在被称为Ks的小型肯扬细胞中，首次发现了这种细胞。这些基因的cDNA没有明显的长阅读框，这表明M5和Ks基因转录本的功能是非翻译的RNA。对蜂蛹脑中M5基因转录本的原位杂交分析表明，蜜蜂MB是一个由四个克隆单位组成的四重结构，每个克隆单位包含两个表达不同基因的不同区域。进一步分析这些基因产物的功能将揭示影响蜜蜂行为的MB内部结构的细分。我们发现，参与细胞内钙信号转导的蛋白基因在蜜蜂的MB中表达上调。相比之下，据报道，在果蝇中，这些基因在大脑皮层无处不在地表达。据报道，神经细胞的钙信号转导系统参与了记忆和学习的建立。因此，似乎钙信号功能的增强可能有助于蜜蜂MB的鼻窦可塑性增强。从昆虫比较神经生物学的角度来看，这些发现将是重要的。较少

项目成果

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Ito,T.：“睾丸特异性 S-II 的基因组织和染色体作图。”

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