Roles of cells in the liver in occurrence of liver dysfunction in vivo

本研究的目的是阐明梗阻性黄疸对肝脏微循环和枯否细胞活性的影响。在C57BL/6小鼠上结扎胆总管，造成梗阻性黄疸。术后1周和2周取材，用活体肝脏显微镜观察白细胞卷曲贴壁和肝窦血流情况。吞噬活性也用FITC标记的珠子进行了定量。胆管结扎后1周和2周，白细胞滚动明显增加，达到对照组的409.5%。肝窦血流量在胆管结扎后随着吞噬活性的增强而显著减少，肝窦血流量以每个显微镜视野内包含血流的肝窦数目来评估。这些数据表明，梗阻性黄疸导致白细胞黏附和Kupffer细胞活性增加，这可能会损害肝脏微循环，使宿主容易发生肝功能衰竭。

The aim of this study was to clarify the effect of obstructive jaundice on hepatic microcirculation and Kupffer cell activities. Common bile duct ligation was performed on C57BL/6 mice to induce obstructive jaundice. One and 2 weeks after the operation, Leukocyte rolling and sticking, and hepatic sinusoidal blood flow were evaluated using hepatic intravital microscopy. Phagocytic activities were also quantitated using FITC-labeled beads. Leukocyte rolling increased significantly 1 and 2 weeks after bile duct ligation upto 409.5 % of control. Hepatic sinusoidal blood flow, evaluated as the number of sinusoids containing blood flow per microscopic field, decreased significantly associated with increased phagocytic activities after bile duct ligation. These data suggest that obstructive jaundice results in increased leukocyte adhesion and Kupffer cell activities, which may impair hepatic microcirculation, and make a host susceptible to hepatic failure.