Study of pathogenesis and treatment in metastatic bone disease

1.转移性骨疾病发病机制的动物模型为了建立骨转移的动物模型，将B-16黑色素瘤细胞接种于C57BL/6小鼠背部，建立了B-16黑色素瘤细胞移植模型。接种肿瘤细胞4周后，皮下种植的骨外骨转移发生率较高。结论：1.该模型具有独到之处，可用于骨转移和血管生成的研究。多胺合成抑制剂甲基乙醛-双环戊基氨基肼(MGBCP)治疗骨转移的实验结果显示，与对照组相比，MGBCP能显著降低骨转移率。其发病机制可能是抑制肿瘤细胞生长和血管生成。本实验系列未发现不良反应。局部热疗治疗转移性骨疾病铁磁场局部热疗对骨转移瘤的生长有明显的抑制作用。

1. Animal model for investigation of pathogenesis in metastatic bone diseaseIn order to establish an animal model for bone metastasis B-16 melanoma cells were injected into the heart after implantation of allogenic bone in the back of a C57BL/6 mouse. Metastasis were found at high frequent in the extra skeletal bones implanted subcutaneously after 4week inoculation of the tumor cells. The original model, being an unique and useful, was applied for the study of progress of bone metastasis and angiogenesis.2. Treatment study of bone metastasisIntraperitoneal administration of Polyamine synthesis inhibitor, Methylglyoxal-bis-cyclopentylamidinohydrazone (MGBCP), showed significant decrease of metastatic rate in the implanted bone, compared to control group. This pathogenesis may be inhibition of tumor cell growth and angiogenesis. No adverse effects were found in this experimental series.3. Treatment by local hyperthermia to metastatic bone diseaseLocal hyperthermia by ferromagnetic field showed a significant inhibitory effect of growth of metastasis in the bone.