Study of pathogenesis and treatment in metastatic bone disease

骨转移病发病机制及治疗研究

• 批准号: 09470313
• 负责人: UCHIDA Atsumasa
• 金额: $ 3.52万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470313/
• 关键词: Bone metastasis; Animal model; Polyamine synthesis inhibition; hyperthermia; 温熱療法; 骨移転; オルニチン脱炭酸酵素; 磁性体

1. Animal model for investigation of pathogenesis in metastatic bone diseaseIn order to establish an animal model for bone metastasis B-16 melanoma cells were injected into the heart after implantation of allogenic bone in the back of a C57BL/6 mouse. Metastasis were found at high frequent in the extra skeletal bones implanted subcutaneously after 4week inoculation of the tumor cells. The original model, being an unique and useful, was applied for the study of progress of bone metastasis and angiogenesis.2. Treatment study of bone metastasisIntraperitoneal administration of Polyamine synthesis inhibitor, Methylglyoxal-bis-cyclopentylamidinohydrazone (MGBCP), showed significant decrease of metastatic rate in the implanted bone, compared to control group. This pathogenesis may be inhibition of tumor cell growth and angiogenesis. No adverse effects were found in this experimental series.3. Treatment by local hyperthermia to metastatic bone diseaseLocal hyperthermia by ferromagnetic field showed a significant inhibitory effect of growth of metastasis in the bone.

1.为建立骨转移动物模型，将B-16黑色素瘤细胞植入C57 BL/6小鼠背部同种异体骨后，注入心脏。肿瘤细胞接种4周后，在皮下植入的额外骨骼中发现高频率的转移。该模型具有独特性和实用性，可用于研究骨转移和血管生成的进展.骨转移的治疗研究腹腔注射多胺合成抑制剂甲基甘氨酰双环戊脒腙（MGBCP），与对照组相比，植入骨的转移率显著降低。其发病机制可能是抑制肿瘤细胞生长和血管生成。在本实验系列中未发现不良反应。局部热疗对骨转移瘤的治疗研究铁磁场局部热疗对骨转移瘤的生长有明显的抑制作用。

项目成果

Nakanishi H: "Immunohistochemical detection of bcl-2 and p53 pr.oteins and apotosis in soft tissue sarcoma:their correlations with prognosis" Oncology. 54. 238-244 (1997)

Nakanishi H：“软组织肉瘤中 bcl-2 和 p53 蛋白质和细胞凋亡的免疫组织化学检测：它们与预后的相关性”肿瘤学。

K.Takegami: "New ferromagnetic bone cement for local hyperthermia" J Biomed Mater Res. 43. 210-214 (1998)

K.Takegami：“用于局部热疗的新型铁磁骨水泥”J Biomed Mater Res。

M Hasegawa: "Repair of osteochondral defect using artificial articular cartilage" Bioceramics. 10. 587-590 (1997)

M Hasekawa：“使用人工关节软骨修复骨软骨缺损”生物陶瓷。

A.Uchida: "Bone and soft tissue sarcomas : Summary of the third united states/Japan clinical trials summit J Cancer Res Clin Oncol" J Cancer Res Clin Oncol. 123. 674-677 (1997)

A.Uchida：“骨和软组织肉瘤：第三届美国/日本临床试验峰会 J Cancer Res Clin Oncol 总结”J Cancer Res Clin Oncol。

K.Takegami: "New ferromagnetic bone cement for local hyperthermia" Bioceramic. 10. 535-538 (1997)

K.Takegami：“用于局部热疗的新型铁磁骨水泥”生物陶瓷。