Gene therapy for the treatment of bone and soft tissue tumors

利用细胞培养和独创的实验动物模型，在体外和体内研究了基因治疗骨和软组织肿瘤的方法。单纯疱疹病毒胸苷激酶基因加抗病毒药物对骨肉瘤肺转移有明显抑制作用。自杀基因疗法也能抑制乳腺癌骨转移的生长。通过差异基因表达方法确定骨肉瘤肺转移相关基因Decorin为富白蛋白小蛋白聚糖家族。在动物模型中，转染decorin基因的骨肉瘤细胞肺转移明显受到抑制。在31例高级别软组织肉瘤中，免疫组化染色显示高表达的decorin预后较好。这些结果提示基因治疗对骨和软组织肿瘤及其肺转移的治疗是有用的。

Gene therapy for the treatment of bone and soft tissue tumors was studied in vitro and in vivo using cell culture and an experimental animal model developed originally. Lung metastases of osteosarcoma were inhibited markedly by addition of thymidine kinase gene of herpes simplex virus followed by anti-virus agent. Growth of bone metastasis due to breast cancer was also inhibited by suicide gene therapy. Decorin, small leucin-rich proteoglycan family, was identified as lung metastasis-related gene in osteosarcoma by differential gene expression methods. Lung metastasis of ostesarcoma cells transfected decorin gene were inhibited significantly in an animal model.In 31 high grade soft tissue sarcomas higher expression decorin by immunohistochemical staining showed better prognosis.These results suggest that gene therapy is useful for the treatment of bone and soft tissue tumors and their lung metastasis.