Gene therapy for the treatment of bone and soft tissue tumors

基因疗法治疗骨和软组织肿瘤

• 批准号: 15390456
• 负责人: UCHIDA Atsumasa
• 金额: $ 9.47万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390456/
• 关键词: bone and soft tissue tumors; gene therapy; suicide gene; osteosarcoma; lung metastasis; decorin; minimum invasive surgery; 軟骨肉腫; 骨転移; 軟部肉腫; 低侵襲手術; 骨軟部肉腫; 軟骨部肉腫

Gene therapy for the treatment of bone and soft tissue tumors was studied in vitro and in vivo using cell culture and an experimental animal model developed originally. Lung metastases of osteosarcoma were inhibited markedly by addition of thymidine kinase gene of herpes simplex virus followed by anti-virus agent. Growth of bone metastasis due to breast cancer was also inhibited by suicide gene therapy. Decorin, small leucin-rich proteoglycan family, was identified as lung metastasis-related gene in osteosarcoma by differential gene expression methods. Lung metastasis of ostesarcoma cells transfected decorin gene were inhibited significantly in an animal model.In 31 high grade soft tissue sarcomas higher expression decorin by immunohistochemical staining showed better prognosis.These results suggest that gene therapy is useful for the treatment of bone and soft tissue tumors and their lung metastasis.

利用细胞培养和最初开发的实验动物模型，在体外和体内研究了用于治疗骨和软组织肿瘤的基因治疗。单纯疱疹病毒胸苷激酶基因联合抗病毒药物对骨肉瘤肺转移有明显的抑制作用。自杀基因治疗也抑制了乳腺癌骨转移的生长。核心蛋白聚糖（Decorin）是一个富含亮氨酸的蛋白聚糖家族，通过基因表达差异分析，确定其为骨肉瘤肺转移相关基因。在动物模型中，转染decorin基因的骨肉瘤细胞肺转移明显受到抑制，在31例高级别软组织肉瘤中，免疫组化染色显示decorin基因高表达者预后较好，提示基因治疗可用于骨和软组织肿瘤及其肺转移的治疗。

项目成果

New cell lines chondrocytic phenotypes from human chondrosarcoma

来自人类软骨肉瘤的新细胞系软骨细胞表型

• 发表时间: 2004
• 期刊: Virchow Arch 444
• 作者: Kudawara I;Araki N;Myoui A;Kato Y;Uchida A;et al.
• 通讯作者: et al.

Case report : Case of clear cell sarcoma surviving with the primary lesion for 20 years afterresection of a metastatic lymph node

病例报告：透明细胞肉瘤切除转移淋巴结后原发病灶存活 20 年的病例

• 发表时间: 2003
• 期刊: Anticancer Res 23
• 作者: Okamura N;Kusuzaki K;Matsumine A;Uchida A. et al.
• 通讯作者: Uchida A. et al.

Tenascin-C levels in pseudosynovial fluid of loose hip prosthesis

松动髋关节假体假滑液中腱蛋白-C 的水平

• 发表时间: 2005
• 期刊: Scand J Rheumatol 34
• 作者: Hasegawa M;Uchida A;et al.
• 通讯作者: et al.

Hasegawa M, Sudo A, Hirata H, Uchida A: "Ceramic acetabular liner fracture in total hip arthroplasty with a ceramic sandwich cup"J Arthroplasty. 18. 658-661 (2003)

Hasekawa M、Sudo A、Hirata H、Uchida A：“陶瓷夹心杯全髋关节置换术中的陶瓷髋臼内衬骨折”J 关节成形术。

Case report : Recurrence of soft tissue MFH in bone due to minute intravenous tumor emboli detected by MRI

病例报告 : MRI 检测到微小静脉肿瘤栓塞导致骨内软组织 MFH 复发

• 发表时间: 2003
• 期刊: Oncol Rep 10
• 作者: Nakamura T;Kusuzaki K;Seto M;Matsumine A;Uchida A
• 通讯作者: Uchida A