A new type of cytokine derived from lymphocytes affecting on nervous-endocrine system : Searching by fuzzy set cloning method

影响神经内分泌系统的一种新型淋巴细胞细胞因子:模糊集克隆法搜索

基本信息

项目摘要

Cytokines were originally reported to be generated during the interaction of lymphocytes with specific antigens or non-specific mitogens and to function as communication signals among the immune systems. To investigate an interaction between lymphocytes and neurons or endocrine cells, and to seek for a new cytokine, the effects of lymphocytes and their conditioned medium on catecholamine efflux were examined in cultured bovine adrenal medullary cells. 1) Co-culture of adrenal medullary cells with lymphocytes for 3 days enhanced catecholamine efflux from the cells. 2) A conditioned medium prepared by incubation of lymphocytes for 3 days also stimulated catecholamine efflux in a time-and concentration-dependent manner. 3) Heat treatment of the conditioned medium at 60℃ and 100℃ reduced stimulatory effect to 59 and 20% of control. The stimulatory activity of the conditioned medium was almost observed in a high molecular fraction after dialysis (<8kDa cutoff) or gel filtration. 4) The cond … More itioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [ィイD13ィエD1H] norepinephrine uptake by the cells. These findings suggest that lymphocytes release a heat-sensitive and high molecular (>8kDa) factor (lymphocyte-derived factor) which stimulates catecholamine efflux from adrenal medullary cells.Lymphocyte-derived factor(s)(LDFs) were partially purified by precipitation with 100% ammonium sulfate and followed by DEAE-cellulose column chromatography. Three peaks of active fraction were collected and pooled. Two antisera against the peak l and peak 2 were prepared and named as fuzzy antibody land antibody 2. Using the fuzzy antibody 1, 20 positive clones were isolated from bovine spleen cDNA library and partially sequenced. In these clones sequenced, the sequences of several clones (NY105, NY10, NY11) were new one when searched by the GENBANK or EMBL databases. The clones of NY16, NY18 and NY5 were found to be a new isoform of IgM, intron region for ornithine decarboxylase and one kind of serine protease, respectively. Less
细胞因子最初被报道是在淋巴细胞与特异性抗原或非特异性有丝分裂原相互作用期间产生的,并作为免疫系统之间的通信信号发挥作用。为了研究淋巴细胞与神经元或内分泌细胞之间的相互作用,寻找新的细胞因子,在培养的牛肾上腺髓质细胞中研究了淋巴细胞及其条件培养液对儿茶酚胺流出的影响。1)肾上腺髓质细胞与淋巴细胞共培养3天可增强细胞的儿茶酚胺流出。2)通过淋巴细胞孵育3天制备的条件培养基也以时间和浓度依赖性方式刺激儿茶酚胺流出。3)60℃和100℃热处理条件培养基的刺激效应分别为对照的59%和20%。条件培养基的刺激活性几乎在透析(<8 kDa截止)或凝胶过滤后的高分子级分中观察到。4)第二, ...更多信息 上述培养液对培养的肾上腺髓质细胞培养液中乳酸脱氢酶的活性和细胞对去甲丙咪嗪敏感的去甲肾上腺素的摄取几乎没有影响。淋巴细胞衍生因子(Lymphocyte-derived factor,LDFs)是一种热敏感的高分子量(> 8 kDa)因子,能刺激肾上腺髓质细胞的儿茶酚胺流出。收集并合并活性级分的三个峰。制备了抗峰1和峰2的两种抗血清,命名为模糊抗体和抗体2。用模糊抗体1从牛脾cDNA文库中筛选出20个阳性克隆,并进行部分序列测定。在测序的克隆中,有几个克隆(NY 105、NY 10、NY 11)的序列在GENBANK或EMBL数据库中检索时为新序列。克隆NY 16、NY 18和NY 5分别为一种新的IgM亚型、鸟氨酸脱羧酶内含子区和一种丝氨酸蛋白酶。少

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yasuhito Uezono: "Adrenomedullin increases intracellular Ca^<2+> and inositol 1,4,5-trisphosphate in human oligodendroglial cell line KG-1C"Brain Research. 786. 230-234 (1998)
Yasuhito Uezono:“肾上腺髓质素增加人少突胶质细胞系KG-1C中的细胞内Ca 2+ 和肌醇1,4,5-三磷酸”脑研究。
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Nobuyuki, Yanagihara: "Stimulatory effect of lymphocyte-derived factor on catecholamine efflux form cultured bovine adrenal medullary cells"Neuroscience Letters. 243. 25-28 (1998)
Nobuyuki, Yanagihara:“淋巴细胞衍生因子对培养的牛肾上腺髓质细胞儿茶酚胺流出的刺激作用”神经科学快报。
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Koji Kajiwara: "Atherogenic lipoproteins inhibit catecholamine secretion in cultured bovine adrenal medullary cells" Naunyn-Schimiedeberg's Archives of Pharmacology. 358. 308-314 (1998)
Koji Kajiwara:“致动脉粥样硬化脂蛋白抑制培养的牛肾上腺髓质细胞中儿茶酚胺的分泌”Naunyn-Schimiedeberg 的药理学档案。
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    0
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Megumi, Aso: "cDNA clonig of inducer reactive protein in adult T-cell leukemia cells by applying the fuzzy cloning theory for sequencing defuzzification"Biomedical Soft Computing and Human Science. 5. 15-20 (1999)
Megumi, Aso:“通过应用模糊克隆理论进行测序去模糊化,对成人 T 细胞白血病细胞中的诱导反应蛋白进行 cDNA 克隆”《生物医学软计算与人类科学》。
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Megumi Aso: "cDNA cloning of inducer reactive protein in adult T-cell leukemia cells by applying the fuzzy set cloning theory for sequencing defuzzification"Biomedical Soft Computing and Human Sciences. 5. 15-20 (1999)
Megumi Aso:“通过应用模糊集克隆理论进行测序去模糊化,在成人 T 细胞白血病细胞中克隆诱导反应蛋白”《生物医学软计算与人类科学》。
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YANAGIHARA Nobuyuki其他文献

Scalar curvature as moment map in generalized Kahler geometry.
标量曲率作为广义卡勒几何中的矩图。
  • DOI:
    10.4310/jsg.2020.v18.n1.a4
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TONDOKORO Tsukumi;NAKATA Akinori;OTSUKA Yasumasa;YANAGIHARA Nobuyuki;ANAN Ayumi;KODAMA Hiromi;SATOH Noriaki;国里愛彦;伊藤正哉;Ryushi Goto
  • 通讯作者:
    Ryushi Goto
アメリカ民主党の変容:バイデン政権が抱える分極化
美国民主党转型:拜登政府面临的两极分化
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TONDOKORO Tsukumi;NAKATA Akinori;OTSUKA Yasumasa;YANAGIHARA Nobuyuki;ANAN Ayumi;KODAMA Hiromi;SATOH Noriaki;渡辺将人
  • 通讯作者:
    渡辺将人
証券市場における流動性の短期間周期性
证券市场流动性的短期周期性
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TONDOKORO Tsukumi;NAKATA Akinori;OTSUKA Yasumasa;YANAGIHARA Nobuyuki;ANAN Ayumi;KODAMA Hiromi;SATOH Noriaki;渡辺将人;村上勇介;太田亘
  • 通讯作者:
    太田亘
心理学研究における研究室インフラの整備
心理学研究实验室基础设施的发展
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TONDOKORO Tsukumi;NAKATA Akinori;OTSUKA Yasumasa;YANAGIHARA Nobuyuki;ANAN Ayumi;KODAMA Hiromi;SATOH Noriaki;国里愛彦
  • 通讯作者:
    国里愛彦
Teacher Education and Training in Japan, Hanoi National University of Education, Vietnam
日本教师教育与培训,越南河内国立教育大学
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    TONDOKORO Tsukumi;NAKATA Akinori;OTSUKA Yasumasa;YANAGIHARA Nobuyuki;ANAN Ayumi;KODAMA Hiromi;SATOH Noriaki;国里愛彦;伊藤正哉;Ryushi Goto;倉本哲男
  • 通讯作者:
    倉本哲男

YANAGIHARA Nobuyuki的其他文献

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{{ truncateString('YANAGIHARA Nobuyuki', 18)}}的其他基金

Influences of plant flavonoids on body functions and basical research for evaluation of functional foods
植物黄酮对机体功能的影响及功能食品评价基础研究
  • 批准号:
    23617035
  • 财政年份:
    2011
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Basic study on a new membrane estrogen receptor and search for its selective agonist
新型膜雌激素受体的基础研究及其选择性激动剂的寻找
  • 批准号:
    20611020
  • 财政年份:
    2008
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Effects of orexin on catecholamine synthesis and release in adrenal medulla
食欲素对肾上腺髓质儿茶酚胺合成和释放的影响
  • 批准号:
    13670855
  • 财政年份:
    2001
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Stimulus-transcription coupling in catecholamine synthesis
儿茶酚胺合成中的刺激-转录耦合
  • 批准号:
    03670115
  • 财政年份:
    1991
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Development of platform for non-invasive, rapid, and simple analysis of cytokine secretion from single cells
开发非侵入、快速、简单分析单细胞细胞因子分泌的平台
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    23H01824
  • 财政年份:
    2023
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    $ 3.2万
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Therapeutic approach to confer an anti-tumorigenic role of IL-6 to the pro-tumorigenic cytokine in effector T cells
赋予效应T细胞中促肿瘤细胞因子IL-6抗肿瘤作用的治疗方法
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    23K06723
  • 财政年份:
    2023
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    $ 3.2万
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    Grant-in-Aid for Scientific Research (C)
Diversity of haematopoietic cytokine receptor activation exploring with marine probes
用海洋探针探索造血细胞因子受体激活的多样性
  • 批准号:
    23K14019
  • 财政年份:
    2023
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    $ 3.2万
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FOXA1 regulates cytokine signaling and immune landscape in prostate cancer through ARID1A
FOXA1 通过 ARID1A 调节前列腺癌中的细胞因子信号传导和免疫景观
  • 批准号:
    10681898
  • 财政年份:
    2023
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Combinatorial cytokine-coated macrophages for targeted immunomodulation in acute lung injury
组合细胞因子包被的巨噬细胞用于急性肺损伤的靶向免疫调节
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    10648387
  • 财政年份:
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Cytokine Regulation of Secondary Neural Progenitors
次级神经祖细胞的细胞因子调节
  • 批准号:
    10752901
  • 财政年份:
    2023
  • 资助金额:
    $ 3.2万
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A point-of-care salivary cytokine test for early detection of oral cancer
用于早期发现口腔癌的即时唾液细胞因子检测
  • 批准号:
    10760626
  • 财政年份:
    2023
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Development of Coccidioides Cytokine Release Assay
球孢子菌细胞因子释放测定的发展
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    10760131
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Generating a novel conditional knockout mouse for a super-enhancer that controls cytokine responsiveness
生成一种新型条件敲除小鼠,用于控制细胞因子反应的超级增强子
  • 批准号:
    10740932
  • 财政年份:
    2023
  • 资助金额:
    $ 3.2万
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Cytokine receptor common beta chain alterations in mediastinal lymphomas
纵隔淋巴瘤细胞因子受体常见β链改变
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    489197
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    $ 3.2万
  • 项目类别:
    Operating Grants
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