A new type of cytokine derived from lymphocytes affecting on nervous-endocrine system : Searching by fuzzy set cloning method

细胞因子最初报告说，在具有特定抗原或非特定突变体的情况下产生淋巴细胞的相互作用，并作为免疫系统中的通信信号发挥作用。为了研究淋巴细胞和神经元或内分泌细胞之间的相互作用，并寻找一种新的细胞因子，其对淋巴细胞及其对儿茶素效应的影响及其调节介质在培养的婴儿肾上腺素医学细胞中进行了测试。（1）与细胞中3天增强的儿茶素效应的肾上腺素结合培养的肾上腺素。（2）一种条件培养基，由淋巴细胞孵化为3天，也是受刺激的儿茶素效应在一个时间和并发依赖性的指示器中制备。（3）在60 ° C和100 ° C对条件介质进行热处理，将刺激效应降低到控制的59和20%。条件介质的刺激活动几乎可以在透析（<8 kDa切割）或凝胶过滤后的高分子片段中观察到。（4）康德 ... More itioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [イイD13エD1H] norepinephrine uptake by the cells。这些发现表明，淋巴细胞释放了一种热敏感的高分子（> 8 kDa）因子（淋巴细胞衍生因子），其中刺激了肾上腺素衍生因子（s）（LDFs）部分地通过预激活了100%氨基硫酸盐和DEAE-Cellulose柱色谱法。三个峰值的活动分数被收集和池化。两种对抗峰值和峰值2的抗氧化剂已经准备好并命名为模糊抗体陆地抗体2。使用模糊抗体1， 20阳性克隆体是从bovine spleen cDNA库和部分测序的。在这些克隆人序列中，几个克隆人序列（NY 105，NY 10，NY 11）是由Genbank或EMBL数据库搜索的新克隆人。在纽约16、纽约18和纽约5的克隆人中发现了一种新的IgM、Intron区域，用于精氨酸decarboxylase和一种丝氨酸蛋白酶，尽管如此。Less（低）

Cytokines were originally reported to be generated during the interaction of lymphocytes with specific antigens or non-specific mitogens and to function as communication signals among the immune systems. To investigate an interaction between lymphocytes and neurons or endocrine cells, and to seek for a new cytokine, the effects of lymphocytes and their conditioned medium on catecholamine efflux were examined in cultured bovine adrenal medullary cells. 1) Co-culture of adrenal medullary cells with lymphocytes for 3 days enhanced catecholamine efflux from the cells. 2) A conditioned medium prepared by incubation of lymphocytes for 3 days also stimulated catecholamine efflux in a time-and concentration-dependent manner. 3) Heat treatment of the conditioned medium at 60℃ and 100℃ reduced stimulatory effect to 59 and 20% of control. The stimulatory activity of the conditioned medium was almost observed in a high molecular fraction after dialysis (<8kDa cutoff) or gel filtration. 4) The cond … More itioned medium had little effect on the activity of lactate dehydrogenase in the medium of cultured adrenal medullary cells and on desipramine-sensitive [ィイD13ィエD1H] norepinephrine uptake by the cells. These findings suggest that lymphocytes release a heat-sensitive and high molecular (>8kDa) factor (lymphocyte-derived factor) which stimulates catecholamine efflux from adrenal medullary cells.Lymphocyte-derived factor(s)(LDFs) were partially purified by precipitation with 100% ammonium sulfate and followed by DEAE-cellulose column chromatography. Three peaks of active fraction were collected and pooled. Two antisera against the peak l and peak 2 were prepared and named as fuzzy antibody land antibody 2. Using the fuzzy antibody 1, 20 positive clones were isolated from bovine spleen cDNA library and partially sequenced. In these clones sequenced, the sequences of several clones (NY105, NY10, NY11) were new one when searched by the GENBANK or EMBL databases. The clones of NY16, NY18 and NY5 were found to be a new isoform of IgM, intron region for ornithine decarboxylase and one kind of serine protease, respectively. Less