Forensic Studies on Identification of a Suspect in a Sexual Crime by Typing of DNA Polymorphisms and Biochemical Genetic Markers

通过DNA多态性和生化遗传标记分型识别性犯罪嫌疑人的法医学研究

• 批准号: 09470122
• 负责人: YASUDA Toshihiro
• 金额: $ 6.4万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470122/
• 关键词: Persona identification; Sexual crime; Deoxyribonuclease; Semen; Genetic polymorphism; Molecular biology; Human genetics; Genetic markers; 血液型; DNA型; 遺伝子; cDNA構造

1. We have previously found human deoxyribonuclease (DNase) II to be a genetic marker present in semen. In this study, we succeeded in cloning of both the complementary and genomic DNAs encoding human DNase II. Furthermore, regional localization of the gene (DNASE2) to 19p13.2-p13.1 was achieved by FISH analysis. This is the first report of the cloning and characterization of the cDNA and gene for mammalian DNase II.2. DNase II levels in human vary depending on whether the individual has the DNASE2ィイD1*ィエD1H or ィイD1*ィエD1L allele. The transient transfection luciferase analysis of the DNase II gene expression permit us to conclude that the A-75G transition in the proximal promoter region causes the allelic difference in the promoter activity of the gene, underlying its genetic polymorphism.3. Human DNase II was composed of three non-identical subunits. From site-directed mutagenesis study, it was found that a simultaneous attachment of a carbohydrate moiety, proteolytic cleavage and the … More inherent signal peptide might be required for subcellular sorting and maturation of the enzyme.4. Since two new alleles, DNASEIィイD1*ィエD15 and ィイD1*ィエD16, of human DNase I polymorphism were identified, DNase I-polymorphism can be classified into 21 different phenotypes. These findings permit the ability of DNase I-polymorphism in personal identification to be further elevated. The type 6 isoenzyme was the first type to be shown to be labile.5. The findings that Caucasian and Negroid populations were classified into several DNase I phenotypes found DNase -polymorphism to be world-wide. Furthermore, we found a geographical north-south decline in DNase I 2 allele frequency in Japanese populations.6. Two short tandem repeat systems, HumTPO and HumLPL, were investigated in Japanese populations by means of newly developed genotyping method. Duplex typing of these systems were found to be very powerful in forensic utility.Considering these findings made in this research project, both DNases I and II have been confirmed to be one of the most useful and effective genetic markers suited for personal identification from forensic semen samples. Less

1. 我们之前发现人类脱氧核糖核酸酶 (DNase) II 是精液中存在的遗传标记。在这项研究中，我们成功克隆了编码人类 DNase II 的互补 DNA 和基因组 DNA。此外，通过 FISH 分析实现了基因 (DNASE2) 到 19p13.2-p13.1 的区域定位。这是哺乳动物 DNase II.2 的 cDNA 和基因的克隆和表征的第一份报告。人类的 DNase II 水平取决于个体是否具有 DNASE2ィイD1*ィエD1H 或ィイD1*ィエD1L 等位基因。 DNase II基因表达的瞬时转染荧光素酶分析使我们得出这样的结论：近端启动子区的A-75G转变导致该基因启动子活性的等位基因差异，这是其遗传多态性的基础。3．人类 DNase II 由三个不同的亚基组成。通过定点诱变研究，发现酶的亚细胞分选和成熟可能需要同时附着碳水化合物部分、蛋白水解裂解和固有信号肽。4．由于人类DNase I多态性的两个新等位基因DNASEIiiD1*ィエD15和ィイD1*ィエD16被鉴定出来，DNase I多态性可分为21种不同的表型。这些发现使得DNase I多态性在个人身份识别中的能力进一步提高。 6 型同工酶是第一个被证明不稳定的类型。5．将白种人和黑人群体分为几种 DNase I 表型的研究发现，DNase 多态性是世界范围内的。此外，我们发现日本人群中 DNase I 2 等位基因频率从北到南呈下降趋势。6。通过新开发的基因分型方法在日本人群中研究了两个短串联重复系统 HumTPO 和 HumLPL。人们发现这些系统的双重分型在法医实用性方面非常强大。考虑到本研究项目中的这些发现，DNA酶 I 和 II 已被证实是最有用和最有效的遗传标记之一，适合从法医精液样本中进行个人识别。较少的

项目成果

Aoyama Masahiro: "DYS19 marker in Japanese populations"International Journal of Legal Medicine. 110(2). 112-113 (1997)

青山正宏：“日本人群中的 DYS19 标记”《国际法律医学杂志》。

Toshihiro Yasuda: "Population studies of human deoxyribonuclease I polymorphism"Human Heredity. 47(3). 121-124 (1997)

Toshihiro Yasuda：“人类脱氧核糖核酸酶 I 多态性的群体研究”人类遗传。

Souichi Tsutsumi: "Phenotype 2 of deoxyribonuclease I may be used as a risk factor of gastric carcinoma"Cancer. 82(9). 1621-1625 (1998)

Souichi Tsutsumi：“脱氧核糖核酸酶 I 的表型 2 可能被用作胃癌的危险因素”癌症。

Tamiko Nakajima: "Two novel sereening methods for selecting monoclonal antibodies which specifically inhibit DNase I enzyme activity"Immunological Investigation. 27(3). 145-152 (1998)

Tamiko Nakajima：“两种新的筛选方法，用于选择特异性抑制 DNase I 酶活性的单克隆抗体”免疫学研究。

Haruo Takeshita: "Identification of the three non-identical subunits constituting human deoxyribonuclease II"FEBS Letters. 440(1/2). 239-242 (1998)

Haruo Takeshita：“构成人脱氧核糖核酸酶 II 的三个不同亚基的鉴定”FEBS 快报。