Forensic Studies on Identification of a Suspect in a Sexual Crime by Typing of DNA Polymorphisms and Biochemical Genetic Markers

1.我们以前已经找到了人类脱氧核糖核酸酶（DNase） II，在这个过程中成为遗传标记。在这项研究中，我们成功地克隆了两种补充DNA和基因组DNA编码人类DNA酶II。Furthermore，基因的区域定位（DNASE 2）至19p13.2 - 13.1通过FISH分析完成。这是对哺乳动物DNA酶II的cDNA和基因克隆和表征的第一份报告。在human vary depending on whether the individual has the DNASE 2中的D1* 中的D1 H or中的D1* 中的D1 L allele。The transient transfection luciferase analysis of the DNase II gene expression permit us to conclude that the A-75 G transition in the proximal promoter region causes the allelic difference in the promoter activity of the gene， underlying its genetic polymorphism. 3.人类DNA酶II是由三个非身份子组成的。从站点指导的突变学研究中，发现了一种类似的附着物，即碳水化合物动力学、蛋白质清除和其他物质。 ... More 固有的信号肽可能需要对酶的亚细胞排序和匹配。Since two new alleles，D1* D15 and D1 * D16， of human？ase I polymorphism were identified，？ase I-polymorphism can be classified into 21 different phenotypes。这些发现允许DNase I的能力-在个人身份识别中，将进一步提升。6型同工酶是第一种被展示出来的异构酶。Caucasian and Negroid populations were classified into several？ase I phenotypes found？ase-polymorphism to be world-wide。Furthermore，我们在日本人口中发现了一个地理学上的北南减线，I-2等位基因频率。两个短串联重复系统， HumTPO和HumLPL，对日本人口进行了调查，这意味着新开发的基因型法。这些系统的双重类型在法医工具中被发现具有非常强大的功能，考虑到这些发现是在这个研究项目中制造的，DNases I和II都被确认为一个由法医semen样本提供的最有用和最有效的遗传标记者。Less（低）

1. We have previously found human deoxyribonuclease (DNase) II to be a genetic marker present in semen. In this study, we succeeded in cloning of both the complementary and genomic DNAs encoding human DNase II. Furthermore, regional localization of the gene (DNASE2) to 19p13.2-p13.1 was achieved by FISH analysis. This is the first report of the cloning and characterization of the cDNA and gene for mammalian DNase II.2. DNase II levels in human vary depending on whether the individual has the DNASE2ィイD1*ィエD1H or ィイD1*ィエD1L allele. The transient transfection luciferase analysis of the DNase II gene expression permit us to conclude that the A-75G transition in the proximal promoter region causes the allelic difference in the promoter activity of the gene, underlying its genetic polymorphism.3. Human DNase II was composed of three non-identical subunits. From site-directed mutagenesis study, it was found that a simultaneous attachment of a carbohydrate moiety, proteolytic cleavage and the … More inherent signal peptide might be required for subcellular sorting and maturation of the enzyme.4. Since two new alleles, DNASEIィイD1*ィエD15 and ィイD1*ィエD16, of human DNase I polymorphism were identified, DNase I-polymorphism can be classified into 21 different phenotypes. These findings permit the ability of DNase I-polymorphism in personal identification to be further elevated. The type 6 isoenzyme was the first type to be shown to be labile.5. The findings that Caucasian and Negroid populations were classified into several DNase I phenotypes found DNase -polymorphism to be world-wide. Furthermore, we found a geographical north-south decline in DNase I 2 allele frequency in Japanese populations.6. Two short tandem repeat systems, HumTPO and HumLPL, were investigated in Japanese populations by means of newly developed genotyping method. Duplex typing of these systems were found to be very powerful in forensic utility.Considering these findings made in this research project, both DNases I and II have been confirmed to be one of the most useful and effective genetic markers suited for personal identification from forensic semen samples. Less