Utilization of DNase I as a risk factor and/or useful diagnostic biomarker for myocardial infarction and gastroenterological cancer

利用 DNase I 作为心肌梗塞和胃肠道癌症的危险因素和/或有用的诊断生物标志物

• 批准号: 23659367
• 负责人: YASUDA Toshihiro
• 金额: $ 2.33万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659367/
• 关键词: DNase I; 心筋虚血; 消化器がん; 自己免疫疾患; DNase family; 診断マーカー; リスクファクター; SNP; DNase II; DNase 1L2; 不安定狭心症; 遺伝的危険因子; 疾患感受性遺伝子; SLE; 遺伝子型―活性相関

From this study, we could demonstrate that;(1) Serum DNase I activity can be used as a useful marker for disease activity and may be involved in the pathogenesis of SLE.(2) A high diagnostic efficacy of serum DNase I activity levels could be observed at the early phase in unstable angina pectoris or non-ST segment elevation myocardial infarction patients who did not show elevation of conventional cardiac biomarkers, suggesting that serum DNase I activity can be a useful biomarkers for the early diagnosis of these diseases inducing a transient myocardial ischemia.(3)Application of a microchip electrophoresis allowed a novel assay method for DNase I activity with a less time-consuming to be developed.(4) In non-synonymous SNPs of DNaseI, II and I-like 3 genes, specific alleles producing an inactive/low activity-harboringenzyme were found. These alleles might be one of several factors involved in genetic predisposition to autoimmune disease.(5)Three rheumatoid arthritis-related SNPs in the promoter region of the DNase II gene could affect in vivo DNase II activity through reduction of the promoter activity.

从这项研究中，我们可以证明；(1)血清DNase I活性可作为疾病活动性的有用标志，可能参与SLE的发病机制。(2)血清DNase I活性水平在不稳定型心绞痛或非st段抬高型心肌梗死患者的早期诊断效能较高，提示血清DNase I活性可作为早期诊断这些疾病引起的短暂性心肌缺血的有用生物标志物。(3)微芯片电泳的应用使得一种新的检测dna酶I活性的方法得以开发，而且耗时更短。(4)在dna ei、II和I-like 3基因的非同义snp中，发现了产生无活性/低活性窝藏酶的特异性等位基因。这些等位基因可能是导致自身免疫性疾病遗传易感性的几个因素之一。(5) DNase II基因启动子区域的三个与类风湿关节炎相关的snp可以通过降低启动子活性来影响体内DNase II的活性。

项目成果

HMGA2遺伝子多型：日本人身長との関連性と民族差異

HMGA2 基因多态性：与日本人身高和种族差异的关联

• 作者: 藤原純子;竹下治男;安田年博ら;飯田 礼子;安田 年博
• 通讯作者: 安田 年博

Serum deoxyribonuclease I activity can be a useful diagnostic marker for the early diagnosis of unstable angina pectoris or non-ST-segment elevation myocardial infarction

• DOI: 10.1016/j.jjcc.2012.01.005
• 发表时间: 2012-05-01
• 期刊: JOURNAL OF CARDIOLOGY
• 影响因子: 2.5
• 作者: Fujibayashi, Kousuke;Kawai, Yasuyuki;Yasuda, Toshihiro
• 通讯作者: Yasuda, Toshihiro

Accuracy and usefulness of the AVOXimeter 4000 in determining circumastances related to cause of death

AVOXimeter 4000 在确定与死亡原因相关的情况方面的准确性和有用性

• 发表时间: 2013
• 期刊: Forensic Toxicology
• 影响因子: 2.2
• 作者: Shinkai S;Toba M;Saito T;Sato I;Tsubouchi M;Taira K;Kakumoto K;Inamatsu T;Yoshida H;Fujiwara Y;Fukaya T;Matsumoto T;Tateda K;Yamaguchi K;Kohda N;Kohno S;Junko Fujihara
• 通讯作者: Junko Fujihara

Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation.

使用基于 DNA 降解的微芯片电泳快速测量脱氧核糖核酸酶 I 活性。

• 发表时间: 2011
• 期刊: Analytical Biochemistry
• 影响因子: 2.9
• 作者: J. Fujihara;M. Tabuchi;Takayuki Inoue;T. Yasuda;Y. Fujita;H. Takeshita
• 通讯作者: H. Takeshita

Five non-synonymous single nucleotide polymorphisms in the gene encoding human deoxyribonuclease I-like 2 implicated in terminal differentiation of keratinocytes reduce or abolish its activity

编码人脱氧核糖核酸酶 I-like 2 的基因中的五个非同义单核苷酸多态性涉及角质形成细胞的终末分化，从而降低或消除其活性

• 发表时间: 2013
• 期刊: Electrophoresis
• 影响因子: 2.9
• 作者: M. Ueki;R. Iida;T. Yasuda ら
• 通讯作者: T. Yasuda ら