Utilization of DNase I as a risk factor and/or useful diagnostic biomarker for myocardial infarction and gastroenterological cancer

从这项研究中，我们可以证明；(1)血清DNase I活性可作为疾病活动性的有用标志，可能参与SLE的发病机制。(2)血清DNase I活性水平在不稳定型心绞痛或非st段抬高型心肌梗死患者的早期诊断效能较高，提示血清DNase I活性可作为早期诊断这些疾病引起的短暂性心肌缺血的有用生物标志物。(3)微芯片电泳的应用使得一种新的检测dna酶I活性的方法得以开发，而且耗时更短。(4)在dna ei、II和I-like 3基因的非同义snp中，发现了产生无活性/低活性窝藏酶的特异性等位基因。这些等位基因可能是导致自身免疫性疾病遗传易感性的几个因素之一。(5) DNase II基因启动子区域的三个与类风湿关节炎相关的snp可以通过降低启动子活性来影响体内DNase II的活性。

From this study, we could demonstrate that;(1) Serum DNase I activity can be used as a useful marker for disease activity and may be involved in the pathogenesis of SLE.(2) A high diagnostic efficacy of serum DNase I activity levels could be observed at the early phase in unstable angina pectoris or non-ST segment elevation myocardial infarction patients who did not show elevation of conventional cardiac biomarkers, suggesting that serum DNase I activity can be a useful biomarkers for the early diagnosis of these diseases inducing a transient myocardial ischemia.(3)Application of a microchip electrophoresis allowed a novel assay method for DNase I activity with a less time-consuming to be developed.(4) In non-synonymous SNPs of DNaseI, II and I-like 3 genes, specific alleles producing an inactive/low activity-harboringenzyme were found. These alleles might be one of several factors involved in genetic predisposition to autoimmune disease.(5)Three rheumatoid arthritis-related SNPs in the promoter region of the DNase II gene could affect in vivo DNase II activity through reduction of the promoter activity.