MECHANISMS OF INTRASPINAL TRANSMISSION OF NOCICEPTIVE INFORMATIONS : QUANTITATIVE EVALUATION OF INTRACELLULAR CALCIUM ION CONCENTRATION

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THE INFLAUX OF CALCIUM IONS HAVE A ROLE IN THE SPINAL PROCESSING OF NOCICEPTIVE INFORMATION FROM PERIPHERAL INFLAMMATION.WE MEASURED IINTRACELLULAR CALCIUM ION CONCENTRATION ([CA2+]I) OF THE SPINAL DORSAL HORN NEURONS IN A MODEL OF CARRAGEENAN-INDUCED INFLAMMATION AND EXAMINED THE RESPONSE OF ([CA2+]I) TO PGE2 AND THE EFFECT OF PROSTAGLANDIN RECEPTOR SUBTYPE EP1 ANTAGONIST.A TRANSVERSE SLICES OF LUMBAR SPINAL CORD WERE PRELOADED WITH FURA-2AM.IN LAMINA II-V OF THEH DORSAL HORN NEURONS ON THE INFLAMED SIDE SHOWED HIGHER ([CA2+]I) THAN THE CONTRALATERAL SIDE.THE RESPONSE OF ([CA2+]I) TO PGE2 WAS GREATER ON THE INFLAMED IN LAMINA III-IV.THE PGE2-INDUCED INCREMENT OF ([CA2+]I) WAS SUPPRESSED BY PERFUSION OF EP1 ANTAGONIST, ONO-8711. INFLAMMTION INDUCES NEURONAL SENSITIZATION TO PGE2 AT THE SPINAL DORSAL HORN, AND THE PGE2-INDUCED INCREMENT OF ([CA2+]I) IS MAINLY MEDIATED THROUGH THE ACTIVATION OF EP1 RECEPTOR.