Biopsychological study on characteristics of stress responses associated with appetitive and aversive behaviors

与食欲和厌恶行为相关的应激反应特征的生物心理学研究

• 批准号: 09410024
• 负责人: NAKAHARA Daiichiro
• 金额: $ 5.7万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09410024/
• 关键词: intracranial self-stimulation; immobilization; glumatic acid; medical prefrontal cortex; glucocorticoid; natural killer cell; T cell; B cell; 前頭眼窩野; テトロドトキシン; 取り組み阻害; 内分泌反応; 免疫反応; マイクロダイアリシス; 嫌悪性行動; 欲求性行動; ストレス; 酵素一蛍光分析; グルココルチコイド

The aim of the present study was to examine the effects of stress associated with appetitive and aversive behaviors on endocrine, immune and neurochemical functions in rats. Appetitive and aversive stimuli used here were intracranial self-stimulation and immobilization, respectively. The results were as follows : (1) Plasma corticosterone levels were determined using a radioimmunoassay. Both self-stimulation and immobilization stress increased corticosterone levels in the blood to a similar degree. (2) Cellular immune responses of splenic lymphocytes to B-cell and T-cell mitogens were measured by [ィイD13ィエD1H]thymidine uptake, and natural killer cell cytotoxicity in splenic lymphocytes was measured against YAC-1 by a[ィイD151ィエD1Cr] release assay. Immobilization suppressed all of these immune responses, whereas self-stimulation did not have any effect on immune function. (3) The effects of appetitive and aversive stimuli on glutamate (GLU) efflux in the medial prefrontal cortex (mPFC) wer … More e assessed using microdialysis. Self-stimulation caused a small decrease in extracellular GLU concentrations followed by a large increase returning to basal levels after cessation of the appetitive manipulation. Immobilization produced a rapid increase in GLU levels which peaked twice, during the period of the stress and when rats were freed from the aversive manipulation. Thus, we observed a differential time course of changes in GLU efflux from the mPFC in response to appetitive and aversive stimuli. Both appetition- and aversion-evoked GLU effluxes were attenuated, but not abolished, by co-perfusion of tetrodotoxin (TTX), a sodium channel inhibitor. These TTX-insensitive GLU effluxes sustained biphasic changes, comparable to that in the normal stimulated-GLU effluxes. On the other hand, TTX-sensitive components of GLU efflux were estimated to increase monophasically and to be very similar in their time courses. The results suggest that the different time course of GLU effluxes in the mPFC induced by appetitive and aversive behaviors is a result of TTX-insensitive GLU efflux. Less

本研究的目的是探讨与食欲和厌恶行为相关的应激对大鼠内分泌、免疫和神经化学功能的影响。此处使用的食欲刺激和厌恶刺激分别为颅内自我刺激和固定。结果如下：（1）用放射免疫法测定血浆皮质酮水平。自我刺激和固定应激都以相似的程度增加了血液中的皮质酮水平。(2)通过[胸腺嘧啶D13]胸苷摄取测定脾淋巴细胞对B细胞和T细胞有丝分裂原的细胞免疫应答，并通过[胸腺嘧啶D151]胸腺嘧啶D1Cr]释放试验测定脾淋巴细胞对YAC-1的自然杀伤细胞毒性。固定抑制了所有这些免疫反应，而自我刺激对免疫功能没有任何影响。(3)本研究观察了食欲和厌恶刺激对内侧前额叶皮层（mPFC）谷氨酸（GLU）流出的影响， ...更多信息 e使用微透析评估。自我刺激引起细胞外GLU浓度的小幅下降，随后在停止食欲操纵后大幅增加，恢复到基础水平。固定产生了一个快速增加的葡萄糖水平达到峰值两次，在此期间的压力，当大鼠从令人厌恶的操作。因此，我们观察到不同的时间过程中的变化，葡萄糖流出的mPFC在食欲和厌恶刺激。食欲和厌恶引起的GLU流出减弱，但没有取消，由共同灌注河豚毒素（TTX），钠通道抑制剂。这些TTX不敏感的GLU流出持续的双相变化，在正常的刺激GLU流出。另一方面，TTX敏感的GLU流出的组件估计增加单相，并在其时间过程中是非常相似的。结果表明，食欲和厌恶行为引起的mPFC中GLU流出的不同时间过程是TTX不敏感的GLU流出的结果。少

项目成果

Daiichiro Nakamura: "Intracranial self-stimulation increases differentially in vivo hydroxylation of tyrosine but similarly in vivo hydroxylation of tryptophan in rat medial prefrontal cortex, nucleus accumbens and striatum"Brain Research. (in press). (20

Daiichiro Nakamura：“颅内自我刺激不同程度地增加了大鼠内侧前额皮质、伏隔核和纹状体中酪氨酸的体内羟基化，但类似地增加了色氨酸的体内羟基化”大脑研究。

中原大一郎: "マイクロダイアリシスによる細胞外物質濃度の測定"日本生理学雑誌. 61(10). 363-375 (1999)

Daiichiro Nakahara：“通过微透析测量细胞外物质浓度”，日本生理学杂志 61(10) 363-375 (1999)。

Minami C, Deng Y, Maruyama W, Takahashi T, Kawai M, Nakahara D, Naoi M: "Generation of reactive oxygen species accounts for cytotoxicity of an endogenous dopaminergic neurotoxin, (R)-N-methylsalsolinol, to differentiated dopaminergic SHSY5Y cells"J Neural

Minami C、Deng Y、Maruyama W、Takahashi T、Kawai M、Nakahara D、Naoi M：“活性氧的产生解释了内源性多巴胺能神经毒素（R）-N-甲基salsolinol对分化的多巴胺能SHSY5Y细胞的细胞毒性”

Nakahara D, Ishida Y, Nakamura M, Kuwahara I, Todaka K, Nishimori, T: "Regional differences in desensitization of c-Fos expression following repeated self stimulation of the medial forebrain bundle in the rat"Neuroscience. 90(3). 1013-1020 (1999)

Nakahara D、Ishida Y、Nakamura M、Kuwahara I、Todaka K、Nishimori、T：“大鼠内侧前脑束重复自我刺激后 c-Fos 表达脱敏的区域差异”神经科学。

Kobayashi K, Noda Y, Matsushita N, Nishii K, Sawada H, Nagatsu T, Nakahara D, Fukabori R, Yasoshima Y, Yamamoto T, Miura M, Kano M, Mamiya T, Miyamoto Y, Nabeshima T: "Modest neuropsychological deficits caused by reduced noradrenaline metabolism in mice h

Kobayashi K、Noda Y、Matsushita N、Nishii K、Sawada H、Nagatsu T、Nakahara D、Fukabori R、Yasoshima Y、Yamamoto T、Miura M、Kano M、Mamiya T、Miyamoto Y、Nabeshima T：“引起的适度神经心理缺陷