Characterization of microRNA-protein complexes involved in Hepatitis C Virus translation regulation by the liver-specific microRNA-122

肝脏特异性 microRNA-122 参与丙型肝炎病毒翻译调节的 microRNA-蛋白质复合物的表征

• 批准号: 92854978
• 负责人: Professor Dr. Michael Niepmann
• 金额: --
• 依托单位: Biochemisches Institut
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/92854978?language=en
• 关键词: Characterization; microRNA; protein; complexes; involved

Hepatitis C Virus (HCV) replicates preferentially in the liver and causes liver cirrhosis and hepatocellular carcinoma. Translation of the positive-strand HCV RNA genome is directed by the Internal Ribosome Entry Site (IRES) in the 5 -UTR. The liver-specific microRNA-122 (miR-122) stimulates HCV translation by acting on two miR-122 target sites in the HCV 5 - untranslated region (5 -UTR). In this project, we want to investigate the regulation of HCV translation by miR-122. The project aims at the analysis of the microRNA-protein (miRNP) complexes involved in the action of miR-122 in HCV translation, the identification of miRNP complex components {probably including Ago proteins) and their functional characterization.

丙型肝炎病毒（HCV）优先在肝脏中复制并导致肝硬化和肝细胞癌。正链 HCV RNA 基因组的翻译由 5-UTR 中的内部核糖体进入位点 (IRES) 指导。肝脏特异性 microRNA-122 (miR-122) 通过作用于 HCV 5 - 非翻译区 (5 -UTR) 中的两个 miR-122 靶位点来刺激 HCV 翻译。在这个项目中，我们想要研究 miR-122 对 HCV 翻译的调节。该项目旨在分析参与 miR-122 在 HCV 翻译中作用的 microRNA-蛋白 (miRNP) 复合物，鉴定 miRNP 复合物成分（可能包括 Ago 蛋白）及其功能表征。