Establishment of in vitro experimental method for biological evaluation of biodegradable magnesium

可生物降解镁生物学评价体外实验方法的建立

• 批准号: 22K12903
• 负责人: 張 麓ルウ
• 金额: $ 2.66万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K12903/
• 关键词: 生分解性マグネシウム; 初期分解挙動; In vitro; In vivo; 表面分析

本研究の目的は，医療応用が期待されている生分解性Mg材料を対象に， In vitro（浸漬）試験とIn vivo（家兎埋植）試験における材料の生分解挙動とその関連性を解明し，生体内のMg分解挙動を適切に再現できるIn vitro試験法を確立することである．令和4年度では、分解初期の挙動を明らかにするために，純Mgを細胞培養液（DMEM+10%FBS）に1日の浸漬，また家兎の大腿骨端部と背部皮下に1日の埋植を実施し，走査電子顕微鏡（SEM）及びX線光電子分光法（XPS）を用いて表面分解生成物の観察と組成分析を行った．浸漬試験及び家兎埋植試験のいずれのMg表面においてMg(OH)2，Ca-phosphateとMgCO3等の分解生成物の存在が確認され，浸漬試験試料表面の分解生成物には亀裂が，埋植試料表面の分析生成物には亀裂に加え局部的な剥離も観察された．また，浸漬試験結果から，Ca-phosphate層は緻密であり保護層としてMg表面の腐食挙動に大きく影響し，その生成は緩衝系の重炭素イオン濃度に依存することが分かった．具体的には，重炭素イオン濃度が低い場合には，Ca-phosphate層が生成しやすく腐食が抑制され，表面が相対的平坦であった；一方，重炭素イオン濃度が高い場合には，Ca-phosphate層が生成し難く腐食が進行し亀裂が生じる．以上，浸漬と埋植の両試験の結果より，重炭素イオン濃度がMgの分解挙動に影響する重要な因子であると結論付けた．これまで長期試験での通説として埋植試験におけるMg腐食率は浸漬試験より低いとされてきたが，本研究成果である初期埋植試験におけるMg腐食率は浸漬試験より高いことが分かった．これは局所的な重炭素イオン濃度の違い，また家兎の活動に伴う大腿骨内の骨髄が流れる動的環境に影響された結果と考えられる．

The purpose of this study is to provide information on biodegradable Mg materials such as In vivo, In vitro (immersion), biodegradable and biodegradable materials. The decomposition of Mg in vivo was rediscovered. The method of In vitro was used to make sure that the cycle was established. In 2004 and in the initial stage of decomposition, the cell culture solution (DMEM+10%FBS) of Mg was immersed on the 1st day, and the bone end of the thigh was implanted subcutaneously on the back of the thigh for 1 day. Electron microanalysis (SEM) and X-ray photoelectron spectroscopy (XPS) were used to analyze the composition of surface decomposition products. Immersion and home embedding methods were used to analyze the surface decomposition products of Mg, such as Mg (OH) 2, CaCaPhosphate, MgCO3, etc., and the decomposition products of the surface decomposition products of the immersion test materials were confirmed. The products of embedded material surface analysis were cracked, and the local peeling test was performed. The results show that the Ca-phosphate temperature is very sensitive to the food consumption on the Mg surface, and the heavy carbon content is dependent on the temperature. The specific temperature, carbon content, temperature, temperature Ca-phosphate scavengers generate spoilage suppressants, and the surface is flat. On the one hand, the heavy carbon temperature is very high, and the Ca-phosphate is used to produce spoilage food. The above, immersion and implantation experiments show that the results are satisfactory. Heavy carbon content, carbon content, Mg decomposition activity, heavy carbon consumption, high carbon content, low temperature, low temperature The results of this study show that in the early stage of the experiment, the rate of Mg saprophyte was impregnated. The results of the study showed that the environmental impact of heavy carbon consumption in the environment associated with osteoporosis in the thigh was tested.