Medical use of protease inhibitors for virus infection

克拉拉胰蛋白酶（Tryptase Clara）是一种特异定位于支气管上皮克拉拉细胞并由其分泌的类型素蛋白酶，可水解激活甲型流感病毒的感染性。在本研究项目中，我们发现迷你纤溶酶和胰蛋白酶Clara在体外也能激活流感病毒的传染性。纤溶酶是由纤溶酶n经尿激酶和弹性酶加工而成。为了确定气道中胰蛋白酶Clara的内源性抑制剂是一种防御性化合物，我们检测了人支气管灌洗液，发现了两种该酶的抑制剂。一种是肺表面活性剂，另一种是抗白细胞蛋白酶抑制剂（ALP）。human -inter-胰蛋白酶抑制剂和尿胰蛋白酶抑制剂对Miniplasmin有抑制作用，而ALP对其无抑制作用。在本研究中，两种宿主蛋白酶，胰蛋白酶Clara和miniplasrmn。它们的内源性抑制剂说明了细胞因子在流感病毒感染调控中的作用。

Tryptase Clara, a uypsin-likc protease localized exclusively in and secreted from Clara cells of the bronchial epithielium proteolytically activates the infectivity of influenia A virus. In this rcsearch project, we found that miniplasmin as well as tryptase Clara also activates infectivity of influenza virus in vitro. Miniptasmin is formed From plasminoge n after processing by urokinase and elastase. To identify the endogenous inhibitor(s) of tryptase Clara in airway as a defensive compounds, we examined human bronchial lavage fluid and found two inhibitors of this enzyme. One was a pulmonary surfactant and the other was antilcukoprotease inhibitor (ALP). Miniplasmin was inhibited by hurnan-inter-alpha-trypsin inhibitor and urinary trypsin inhibitor but not by ALP.In this reseach, the two host proteases, tryptase Clara and miniplasrmn. and their endogerious inhibitors illustrate the role of cellular factors in the regulationof influenza virus infection.