Traditional Medicine in Vietnam, Thailand and Myanmar -Investigation on Natural Drug Resources-

1) 1997年，为了了解越南传统医学的历史和现状、药用植物的种植以及传统药物的临床制剂，我们访问了河内和胡志明市的药科大学药物研究所、传统医学研究所、附属药物植物园和大学医院。我们还调查了大叻中部沙巴北部和古布隆的当地民间医药和药用植物。1998年，我们调查了安江省南部和广南省中部。从南北方地区共采集到650种药材和350种药用植物。对越南桂皮、木香和越南人参的栽培、生产和质量控制进行了重点研究。我们还参加了传统医学协会和寺庙的医疗活动。2)在缅甸，对仰光和曼德勒的传统医学进行了详细的调查，收集了100个原料药样本。[2]国内研究1)对在越南、缅甸和泰国采集的药材进行了澄清鉴定。2)制备各药材的水提物和甲醇提物，筛选其保肝和抗转移活性。四棱草叶甲醇提取物对d -半乳糖胺(D-GalN)/脂多糖诱导的小鼠肝损伤具有保护肝活性，对小鼠高肝转移结肠癌26-L5细胞具有细胞毒性。化学鉴定分离到40个化合物，其中新化合物28个。在分离的化合物中，一些三萜具有有效的肝保护活性和D-GalN/TNF-α-诱导原代小鼠肝细胞死亡。从该植物的种子中还分离出了几种保护肝脏的化合物。从白莲种子中分离鉴定了含有查尔酮或黄酮类化合物的新框架芳基七烷类化合物。这些化合物对活化的巨噬细胞产生一氧化氮（NO）有较强的抑制作用。calyxin F和calyxin I对结肠26-L5细胞也表现出抗增殖活性。从雄蕊正虹吸管的地上部分分离到新的碳骨架二萜。这些化合物对转移性结肠26-L5细胞具有细胞毒性。3)对越南人参、越南桂皮、缅甸黄连进行质量评价。少

1] Overseas Investigation1) In 1997, in order to understand the history and current state of traditional medicine, the cultivation of medicinal plants as well as the clinical preparations of traditional medicines in Vietnam, we visited the Institute of Materia Medica, Institute of Traditional Medicine, Medical and Pharmaceutical University, the affiliated Pharmaceutical Botanic Garden and University Hospitals at Hanoi and Hochininh. We also investigated the local traditional folk medicine and medicinal plants at the northern Sapa and Cucphuong, the middle Dalat. In 1998, we investigated the southern Angiang Province and the central Quangnam Province. A total of 650 crude drugs and 350 medicinal plants were collected from the southern and northern regions. The cultivation, manufacture and quality control of Vietnam Cinnamomi Cortex, Aquilariae Lignum and Vietnam Ginseng were particularly investigated. We also participated in the medical events of the traditional medicine associations an … More d temples.2) At Myanmar, after a detailed investigation of the traditional medicine in Yangon and Mandalay, 100 crude drug samples were collected. We also collected 46 samples in Thailand.2] Domestic Research1) The crude drugs collected in Vietnam, Myanmar and Thailand were clarified and identified.2) The water and methanol extracts of each crude drug were prepared and screened for their hepatoprotective and antimetastasis activities. The methanol extract of the leaves of Combretum quadrangulare showed hepatoprotective activity against D-galactosamine(D-GalN)/lipopolysaccharide-induced liver injury in mice and cytotoxicity towards highly liver-metastatic murine colon 26-L5 carcinoma cells. Chemical investigation led to the isolation of 40 compounds including 28 new compounds. Amoung the isolated compounds, some of the triterpenes possessed potent hepatoprotective activity an D-GalN/TNF-α-induced cell death in primary mouse hepatocytes. From the seeds of this plant, several hepatoprotective compounds were also isolated. From the seeds of Alpinia blepharocalyx, arylheptanoids in novel frame with chalcone or flavones moiety were isolated and identified. These compounds showed strong inhibitory effects on nitric oxide (NO) production in activated macrophages. Epicalyxin F and calyxin I also exhibited antiproliferative activity against colon 26-L5 cells. From the aerial part of Orthosiphon staminenus, new diterpenes with novel carbon framework were isolated. These compounds had cytotoxicity against the metastatic colon 26-L5 cells.3) The quality evaluation of Vietnam Ginseng, Vietnam Cinnamomi Cortex and Myanmar Coptis Radix was performed. Less