Traditional Medicine in Vietnam, Thailand and Myanmar -Investigation on Natural Drug Resources-

越南、泰国、缅甸传统医学-天然药物资源调查-

• 批准号: 09041177
• 负责人: KADOTA Shigetoshi
• 金额: $ 4.93万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09041177/
• 关键词: Traditional Medicine; Canser Metastasis; Hepatic Disorder; Vietnam Cinnamomi Cortex; Alpinia blepharocalyx; Combretum quadrangulare; Orthosiphon stamineus; Myanmar Coptis Radix; ベトナム人参; 糖尿病; Combretum guadrangulare; 三七人参

1] Overseas Investigation1) In 1997, in order to understand the history and current state of traditional medicine, the cultivation of medicinal plants as well as the clinical preparations of traditional medicines in Vietnam, we visited the Institute of Materia Medica, Institute of Traditional Medicine, Medical and Pharmaceutical University, the affiliated Pharmaceutical Botanic Garden and University Hospitals at Hanoi and Hochininh. We also investigated the local traditional folk medicine and medicinal plants at the northern Sapa and Cucphuong, the middle Dalat. In 1998, we investigated the southern Angiang Province and the central Quangnam Province. A total of 650 crude drugs and 350 medicinal plants were collected from the southern and northern regions. The cultivation, manufacture and quality control of Vietnam Cinnamomi Cortex, Aquilariae Lignum and Vietnam Ginseng were particularly investigated. We also participated in the medical events of the traditional medicine associations an … More d temples.2) At Myanmar, after a detailed investigation of the traditional medicine in Yangon and Mandalay, 100 crude drug samples were collected. We also collected 46 samples in Thailand.2] Domestic Research1) The crude drugs collected in Vietnam, Myanmar and Thailand were clarified and identified.2) The water and methanol extracts of each crude drug were prepared and screened for their hepatoprotective and antimetastasis activities. The methanol extract of the leaves of Combretum quadrangulare showed hepatoprotective activity against D-galactosamine(D-GalN)/lipopolysaccharide-induced liver injury in mice and cytotoxicity towards highly liver-metastatic murine colon 26-L5 carcinoma cells. Chemical investigation led to the isolation of 40 compounds including 28 new compounds. Amoung the isolated compounds, some of the triterpenes possessed potent hepatoprotective activity an D-GalN/TNF-α-induced cell death in primary mouse hepatocytes. From the seeds of this plant, several hepatoprotective compounds were also isolated. From the seeds of Alpinia blepharocalyx, arylheptanoids in novel frame with chalcone or flavones moiety were isolated and identified. These compounds showed strong inhibitory effects on nitric oxide (NO) production in activated macrophages. Epicalyxin F and calyxin I also exhibited antiproliferative activity against colon 26-L5 cells. From the aerial part of Orthosiphon staminenus, new diterpenes with novel carbon framework were isolated. These compounds had cytotoxicity against the metastatic colon 26-L5 cells.3) The quality evaluation of Vietnam Ginseng, Vietnam Cinnamomi Cortex and Myanmar Coptis Radix was performed. Less

1]海外考察1）1997年，为了了解越南传统医药的历史和现状、药用植物的种植以及传统医药的临床制剂，我们访问了药物研究所、传统医药研究所、医药大学、附属药用植物园和河内和胡志明市的大学医院。我们还调查了北方沙巴和Cucphuong，中部大叻的当地传统民间医药和药用植物。1998年，我们调查了南部的安江省和中部的广南省。从南部和北方地区共收集到650种生药和350种药用植物。对越南沉香、越南人参的栽培、加工及质量控制进行了研究。我们还参加了传统医药协会的医疗活动， ...更多信息 d寺庙。2）在缅甸，在对仰光和曼德勒的传统药物进行详细调查后，收集了100个生药样品。国内研究1）对越南、缅甸、泰国等地的药材进行了澄清和鉴定; 2）对各药材的水提物和甲醇提取物进行了提取，并对提取物的保肝活性和抗肿瘤转移活性进行了筛选。四棱风柴叶的甲醇提取物对D-半乳糖胺（D-GalN）/脂多糖诱导的小鼠肝损伤显示出肝保护活性，并且对高度肝转移的小鼠结肠26-L5癌细胞显示出细胞毒性。通过化学分析，共分离得到40个化合物，其中28个为新化合物。在分离的化合物中，一些三萜类化合物对D-GalN/TNF-α诱导的原代小鼠肝细胞死亡具有有效的保肝活性。从该植物的种子中，还分离出几种保肝化合物。从眼萼山姜种子中分离并鉴定了一系列结构新颖的含查尔酮或黄酮的芳庚类化合物。这些化合物对活化的巨噬细胞产生一氧化氮（NO）有很强的抑制作用。表萼素F和萼素I也表现出对结肠26-L5细胞的抗增殖活性。从毛管草地上部分分离得到具有新碳骨架的二萜类化合物。这些化合物对转移性结肠癌26-L5细胞具有细胞毒性。3）对越南人参、越南丹皮和缅甸黄连进行了质量评价。少

项目成果

Gewali M. B. 他: "Epicalyxin F and Calyxin I ; Two Novel Antiproliferative Diarylheptanoids from the Seeds of Alpinia blepharocalyx"Org. Lett.. 1. 1733-1736 (1999)

Gewali M. B. 等人：“Epicaryxin F 和 Calyxin I ；来自高良姜种子的两种新型抗增殖二芳基庚烷”Org. 1. 1733-1736 (1999)。

Banskota A. H. 他: "Hepatoprotective Effect of Leaves of Combretum quadrangulara and Its Constituents"Biol. Pharm. Bull.. 23(印刷中). (2000)

Banskota A. H. 等人：“四角风车草叶及其成分的肝保护作用”Biol. Bull.. 23（出版中）。

Stampoulis P, Tezuka Y, Banskota A.H., Tran K.Q., Saiki I, Kadota S: "Staminol A, a Novel Diterpene from Orthosiphon Stamineus"Tetrahedron Lett.. 40. 4239-4242 (1999)

Stampoulis P、Tezuka Y、Banskota A.H.、Tran K.Q.、Saiki I、Kadota S：“Staminol A，一种来自 Orthosiphon Stamineus 的新型二萜”Tetrahedron Lett.. 40. 4239-4242 (1999)

Banskota A. H. 他: "Cytotoxic Cycloartane-type Triterpenes from Combretum quadrangulare"Bioorg. Med. Chem. Lett.. 8. 3519-3524 (1998)

Banskota A. H. 等人：“来自风车草的细胞毒性环阿麻型三萜”Bioorg.Med. 8. 3519-3524 (1998)

Prasain J.K., Tezuka Y, Li J.-X., Tnanak K, Basnet P, Dong H, Namba T, Kadota S: "Six novel Diaryiheptanoids Bearing Chalcone or Flavanone Moiety from the seeds of Alpinia blepharocalyx"Tetrahedron. 53. 7833-7842 (1997)

Prasain J.K.、Tezuka Y、Li J.-X.、Tnanak K、Basnet P、Dong H、Namba T、Kadota S：“六种新颖的 Diaryiheptanoids 含有来自 Alpinia blepharocalyx 种子的查尔酮或黄烷酮部分”四面体。