权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Surveillance of Lyme disease borreliae and vector ticks

莱姆病疏螺旋体和媒介蜱的监测

基本信息

批准号：
10041185
负责人：
FUKUNAGA Masahito
金额：
$ 1.73万
依托单位：
Fukuyama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10041185/
关键词：
Borrelia Tick vector Ixodes holocyclus Lyme disease in Australia 適応

项目摘要

We have isolated and characterizedspirochete from skin sample of Australian Lyme disease patient in Sydney area. The isolate was closely related with European Borrelia garinii by comparing its flagellin and rDNA sequences. The outcome indicated that Lyme disease borreliae may also distributed in Australia. We, therefore, investigated tick distribution in Sydney area and infestation of borreliae in Australian tick, Ixodes holocyclus. An internal transcribed spacer (ITS2) sequence between the 5.8S and 28S rRNA genes and mitochondrial rrs sequence were used to estimate the phyletic relationship among Lxodes spp. Tick vectors of Lyme disease-causing Borrelia spirochetes. We found spirochetes in 5% of the tick-mid-gut tissues examined but they did not grow in BSKII medium. PCR amplification using known primer sets for 16S rDNA and/or flagellin gene sequence was also inactive against such tick mid-gut tissue samples. Phyletic analysis by ITS2 sequence indicated that I. Horocyclus is far from the other tick species associated with Lyme disease borreliae. All of out results indicate (1) unknown species of spirochetes is infest in Australian ticks, I. Holocyclus (2) they appear to have a specific association with vector tick species (3) it is not known to transmit the Borrelia-that cause Lyme disease in humans. There is a high degree of concordance in the phylogenetic of Borrelia taxa and the phylogenetic relationships among Oxides ticks, which suggests a history of cospeciation in the tick-spirochete assemblage. The results suggest that the occurrence and distribution of Borrelia in Australia are depend on the vector competence of particular tick species.

我们从悉尼地区的澳大利亚莱姆病患者的皮肤样本中分离并鉴定了螺旋体。通过鞭毛蛋白和rDNA序列比较，该分离株与欧洲疏螺旋体有较近的亲缘关系。结果表明，莱姆病疏螺旋体也可能在澳大利亚分布。因此，我们调查了悉尼地区硬蜱的分布和澳大利亚硬蜱中疏螺旋体的感染情况。利用5.8S和28S rRNA基因之间的内部转录间隔区(ITS2)序列和线粒体RRS序列分析了Lxodes spp之间的亲缘关系。引起莱姆病的螺旋体的扁虱媒介。我们在检查的扁虱中肠组织中发现了5%的螺旋体，但它们不能在BSKII培养基中生长。用已知的16S rDNA和/或鞭毛基因序列的引物组进行的聚合酶链式反应对这种硬蜱中肠组织样本也是无效的。ITS2序列的系统发育分析表明，该种与其他与莱姆病疏螺旋体有关的硬蜱物种有很大的不同。所有结果表明：(1)未知种类的螺旋体寄生在澳大利亚硬蜱I中。(2)它们似乎与媒介硬虱物种有特定的联系。(3)目前尚不清楚是否传播引起人类莱姆病的疏螺旋体。疏螺旋体分类群的系统发育与硬壳类之间的系统发育关系高度一致，表明硬体-螺旋体组合中存在着共分类的历史。结果表明，疏螺旋体在澳大利亚的发生和分布取决于特定种类的媒介能力。