Cooperative Study on Pathogen of Corn Food Poisoning.

玉米食物中毒病原合作研究。

• 批准号: 10044257
• 负责人: EZAKI Takayuki
• 金额: $ 1.73万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044257/
• 关键词: Burholderia cocovenenans; Burkholderia gladioli; 16S rDNA; DNA; DNA hybridization; Detection; Burkholderia gladioli; 16SrRNA; とうもろこし食中毒; 分類

Causative agent of fermented corn food poisoning was once classified as Pseudomonas cocovenenans. The organism was recently transferred to genus Burkholderia. Taxonomic position of B.cocovenenans was still not clear before we start our work. Through this study we analyzed 16 S ribosomal RNA sequences of this organism and found the phylogenentic position, The organism had almost identical 16S ribosomal DNA sequences to the sequence of Burkholderia gladioli. Quantitative DNA/DNA hybridization experiment also revealed that B, cocovenerans and B. gladioli were taxonomically identical because their DNA/DNA similarity values were higher than 70%. However, strains identified as B. cocovenenans produced leatha toxin, banllekic acid. However, human clinical strain and environmental strains identified as B. gladioli did not produce banklekic acid. Therefore, we decided to differentiated these toxin positive and negative strains by pathovars: Toxin positive strains as B. gladioli pathovar cocovenenans and toxinnegative strains as B. gladioli pathovar gladioli. Through survey in Chaina, more death ration of foodpoisoning cases almost reached to 50%, So we designed rapid detection system of B. gladioli pathovar cocovenenans. From sequenced 16s ribosomal DNA, we designed primers to detect B. gladioli, Monoclonal antibody to detect O antigen of this B, gladioli pathovar cocovenerans was prepared. However, gene clusters for banklekic acid synthesis still remains unknown.

发酵玉米食物中毒的致病菌曾被归为椰毒假单胞菌。该微生物最近被转移到伯克霍尔德氏菌属。B.cocovenenans的分类地位尚不明确。通过本研究，我们分析了该生物的16 S核糖体RNA序列，发现了该生物的系统发育位置，该生物的16 S核糖体DNA序列与唐菖蒲伯克霍尔德菌的序列几乎相同。DNA/DNA定量杂交实验还表明，B、椰毒蛋白和B.唐菖蒲的DNA/DNA相似性值高于70%，因此它们在分类上是相同的。然而，菌株被鉴定为B。椰毒壳菌产生的毒素为leatha毒素、banllekic酸。然而，人类临床菌株和环境菌株鉴定为B。唐菖蒲不产生班克酸。因此，我们决定通过致病型来区分这些毒素阳性和阴性菌株：毒素阳性菌株为B。毒力阴性菌株为B.唐菖蒲通过对我国的调查，发现我国食物中毒死亡率高达50%以上，为此我们设计了B快速检测系统。唐菖蒲根据16 s核糖体DNA序列设计引物检测B。制备了检测唐菖蒲椰毒致病变种B O抗原的单克隆抗体。然而，用于banklekic酸合成的基因簇仍然是未知的。

项目成果

Jiao, ZQ, et al.: "Emended description of Burkholderia gladiol and identification of five pathovars by 16S rRNA sequence manuscript in prepation."manuscript in prepation.

Jiao, ZQ, et al.：“正在准备中的伯克霍尔德氏菌剑兰描述和通过 16S rRNA 序列手稿鉴定五种致病菌”手稿正在准备中。

Jiao.ZQ, et al: "Emended description of Burkholderia giadiol and identification of five pathovars by 16srRNA sequence manuscript in Prepation"International Journal of Systematic Bacteriology. (投稿予定).

Jiao.ZQ，等人：“《国际系统细菌学杂志》中的Burkholderia giadiol的修改描述和通过16srRNA序列鉴定五种致病菌的手稿”（待提交）。

Jiro.ZQ, et al.: "Emended description of Burkholderia gladioli and identification of five pathovars by 16SrRNA sequence"International.Journal of Systematic Bacteriology. (投稿予定).

Jiro.ZQ等人：“剑兰伯克霍尔德氏菌的修改描述和通过16SrRNA序列鉴定五种致病菌”国际系统细菌学杂志（待提交）。