Cooperative Study on Pathogen of Corn Food Poisoning.

玉米食物中毒病原合作研究。

• 批准号: 10044257
• 负责人: EZAKI Takayuki
• 金额: $ 1.73万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044257/
• 关键词: Burholderia cocovenenans; Burkholderia gladioli; 16S rDNA; DNA; DNA hybridization; Detection; Burkholderia gladioli; 16SrRNA; とうもろこし食中毒; 分類

Causative agent of fermented corn food poisoning was once classified as Pseudomonas cocovenenans. The organism was recently transferred to genus Burkholderia. Taxonomic position of B.cocovenenans was still not clear before we start our work. Through this study we analyzed 16 S ribosomal RNA sequences of this organism and found the phylogenentic position, The organism had almost identical 16S ribosomal DNA sequences to the sequence of Burkholderia gladioli. Quantitative DNA/DNA hybridization experiment also revealed that B, cocovenerans and B. gladioli were taxonomically identical because their DNA/DNA similarity values were higher than 70%. However, strains identified as B. cocovenenans produced leatha toxin, banllekic acid. However, human clinical strain and environmental strains identified as B. gladioli did not produce banklekic acid. Therefore, we decided to differentiated these toxin positive and negative strains by pathovars: Toxin positive strains as B. gladioli pathovar cocovenenans and toxinnegative strains as B. gladioli pathovar gladioli. Through survey in Chaina, more death ration of foodpoisoning cases almost reached to 50%, So we designed rapid detection system of B. gladioli pathovar cocovenenans. From sequenced 16s ribosomal DNA, we designed primers to detect B. gladioli, Monoclonal antibody to detect O antigen of this B, gladioli pathovar cocovenerans was prepared. However, gene clusters for banklekic acid synthesis still remains unknown.

玉米发酵食物中毒的病原一度被归类为玉米叶假单胞菌。这种有机体最近被转移到伯克氏菌属。cocovenenans的分类地位在我们开始工作之前还不清楚。本研究对该菌的16S核糖体RNA序列进行了分析，确定了该菌的系统发育位置，该菌的16S核糖体DNA序列与剑兰伯克霍尔德菌的序列几乎相同。定量DNA/DNA杂交实验也表明，白桦、白桦和剑兰在分类上是相同的，它们的DNA/DNA相似性值都大于70%。然而，被鉴定为cocovenenans的菌株产生皮革毒素，亚麻酸。然而，人类临床菌株和环境菌株鉴定为b剑兰不产生银柳酸。因此，我们决定将毒素阳性菌株和阴性菌株按病原进行区分：毒素阳性菌株为剑兰白僵菌（B. gladioli pathovar cocovenenans），毒素阴性菌株为剑兰白僵菌。通过在中国的调查，食物中毒病例的死亡率几乎达到50%，因此我们设计了剑兰白僵菌的快速检测系统。从16s核糖体DNA序列中，设计了检测剑兰B型病原菌O抗原的引物，制备了用于检测剑兰B型病原菌O抗原的单克隆抗体。然而，banklekacid合成的基因簇仍然未知。

项目成果

Jiao, ZQ, et al.: "Emended description of Burkholderia gladiol and identification of five pathovars by 16S rRNA sequence manuscript in prepation."manuscript in prepation.

Jiao, ZQ, et al.：“正在准备中的伯克霍尔德氏菌剑兰描述和通过 16S rRNA 序列手稿鉴定五种致病菌”手稿正在准备中。

Jiao.ZQ, et al: "Emended description of Burkholderia giadiol and identification of five pathovars by 16srRNA sequence manuscript in Prepation"International Journal of Systematic Bacteriology. (投稿予定).

Jiao.ZQ，等人：“《国际系统细菌学杂志》中的Burkholderia giadiol的修改描述和通过16srRNA序列鉴定五种致病菌的手稿”（待提交）。

Jiro.ZQ, et al.: "Emended description of Burkholderia gladioli and identification of five pathovars by 16SrRNA sequence"International.Journal of Systematic Bacteriology. (投稿予定).

Jiro.ZQ等人：“剑兰伯克霍尔德氏菌的修改描述和通过16SrRNA序列鉴定五种致病菌”国际系统细菌学杂志（待提交）。