Estrogen receptor alpha signaling in endothelial cells exacerbates arterial stiffening via upregulation of ENaC in insulin resistant females

内皮细胞中的雌激素受体 α 信号传导通过上调 ENaC 加剧胰岛素抵抗女性的动脉硬化

• 批准号: 10152374
• 负责人: Camila Margarita Manrique Acevedo
• 金额: $ 85.84万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10152374
• 关键词: Ablation; Affect; Age; Aging; Aldosterone; Amiloride; Attenuated; Biological Availability; Blood Vessels; Cardiovascular Diseases; Cell Culture Techniques; Cell Volumes; Cell surface; Characteristics; Data; Endothelial Cells; Endothelium; Estrogen Receptor alpha; Estrogens; Fatty acid glycerol esters; Female; Fructose; Functional disorder; Glucocorticoids; Human; Hypertension; In Vitro; Insulin Resistance; Knockout Mice; Measures; Mediating; Menopause; Mus; Nitric Oxide; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Overnutrition; Pathway interactions; Pharmacology; Phosphotransferases; Play; Postmenopause; Predisposition; Premenopause; Process; Protein-Serine-Threonine Kinases; Regulation; Risk; Role; Serum; Sex Differences; Signal Pathway; Signal Transduction; Small Interfering RNA; Stimulus; Therapeutic Agents; Tissues; Translating; Up-Regulation; Woman; Work; arterial stiffness; base; cardiovascular disorder risk; cohort; diabetic; dietary control; endothelial dysfunction; epithelial Na+ channel; experimental study; feeding; high risk; improved; in vivo; inhibitor/antagonist; knockout animal; male; men; mouse model; premature; prevent; protective effect; randomized placebo controlled trial; sex; steroid hormone; translational study; western diet

Project Summary/Abstract Arterial stiffening is a hallmark of the aging process. However, premature stiffening is often seen in hypertension, obesity, insulin resistance, and type 2 diabetes (T2D). Both men and women are affected, but women with T2D are at greater risk. As augmented arterial stiffness is an independent predictor of cardiovascular disease (CVD), the increased susceptibility of insulin-resistant (IR) and obese women to arterial stiffening may explain their higher risk for CVD. In healthy women, estrogen signaling via estrogen receptor alpha (ERα) prevents stiffening, but these effects are blunted in over-nutrition and obesity when the presence of the ER may be deleterious. Endothelial cell (EC) epithelial sodium channel (ENaC) expression increases in obese and IR female mice. Increased EC ENaC contributes to arterial stiffening and EC dysfunction, in part by lowering nitric oxide (NO) bioavailability. Aldosterone is a major ENaC stimulus, but other steroid hormones, specifically estrogen, also promote ENaC expression/function in various tissues. ENaC activity is regulated by the serum glucocorticoid inducible-kinase 1 (SGK-1) pathway. Elevated aldosterone and decreased NO bioavailability are characteristics of obese, IR and T2D women. Based on our prior work and most recent preliminary data, our hypothesis is that estrogen action through the EC ERα upregulates EC ENaC expression and activity, via SGK-1, exacerbating endothelial and arterial stiffening in obese, IR premenopausal females. The corollary to this hypothesis is that ENaC inhibition will have a greater impact on arterial stiffness in obese, premenopausal IR women than in obese IR postmenopausal women or age-matched obese, IR men. Consequently, ENaC inhibition will have a greater impact on arterial stiffness in premenopausal obese, IR women than in obese, IR men. We will measure arterial/EC stiffness in IR and obese EC-specific ERα and ENaC KO mice; in isolated ECs; and in a cohort of obese, IR women (pre and post-menopausal) and age- matched men, to accomplish the following Aims: 1) To determine whether EC ERα regulation of EC ENaC, via SGK-1, plays an important role in the genesis of accelerated endothelial and arterial stiffening in IR female mice and 2) to determine whether treatment with the ENaC inhibitor, amiloride, improves endothelial function and arterial stiffness in obese IR subjects in a randomized placebo-controlled trial. To date, the specific role of EC ERα regulation of ENaC expression/activation, in ensuing sex-related differences in arterial stiffness in obesity and insulin resistance, remains unexplored. This proposal aims to fill that gap and show that targeting ENaC activation holds extraordinary promise in reversing endothelial dysfunction and arterial stiffness in obesity and insulin resistance, and ultimately preventing cardiovascular disease, especially in women.

项目总结/文摘