Biomimetic Wearable Sensors for Alcohol Monitoring

项目摘要 根据2015年全国药物使用和健康调查（NSDUH），1510万成年人使用酒精 在美国的混乱。酒精中毒已被确定为疾病、残疾和 死亡率，带来巨大的经济和社会负担。实时、准确和谨慎的设备， 血液酒精浓度（BAC）的评估是必不可少的授权医学研究与可靠的 可靠的手段来监测酒精行为，以及有效的个人自我评估。测量 通过可穿戴设备通过皮肤渗出的乙醇特别令人感兴趣，因为它可能 对BAC进行谨慎和不显眼的监测，但现有设备仍然缺乏可靠性、准确性和可靠性。 和敏感性。在此背景下，Bioinspira，Inc.开发了一种多功能的基于噬菌体的比色法 传感器平台技术用于气体传感。这种新颖的内部开发的技术使用了一种自组装技术， 噬菌体传感器阵列（M13噬菌体），可通过基因工程与特定气体结合 分子，并根据气体类型和浓度改变其颜色模式。通过利用这种创新的 技术，通过这项SBIR第一阶段研究Bioinspira将评估实现可穿戴设备的可行性， 用于经皮酒精监测的仿生传感器（BiosTAM），用于实时监测乙醇蒸汽， 通过皮肤蒸发。初步实验室数据显示，BioInspira的平台技术 来检测乙醇开发一种具有高灵敏度的噬菌体乙醇传感器将填补我国的技术空白， 酒精行为监测，使更全面和详细的医学研究，并将提供 监测和管理个人酒精使用障碍的可能性。在SBIR第一阶段， 将评估选择性地、灵敏地和实时地检测乙醇蒸气。为此，以下研发 活动内容包括：1.产生和评价用于选择性噬菌体阵列的候选物， 乙醇蒸气的灵敏检测; 2.校准用于噬菌体的最佳性能噬菌体传感器候选物 不同温度和相对湿度条件下乙醇浓度的检测。结果 获得的将是SBIR第二阶段的准备，在那里将进行临床试验， 使用BAC进行透皮酒精测量。 关键词：酒精中毒，酒精滥用，可穿戴式生物传感器，噬菌体，出汗乙醇，酒精蒸汽。

PROJECT SUMMARY According to the 2015 National Survey of Drug Use and Health (NSDUH), 15.1 million adults had alcohol use disorder in the United States. Alcoholism has been identified as an important risk factor for illness, disability, and mortality, carrying a huge economic and societal burden. Real-time, accurate and discreet devices for the assessment of Blood Alcohol Concentration (BAC) are essential to empower medical research with reliable reliable means to monitor alcohol behavior, as well as for effective personal self-assessment. Measurement of ethanol perspired through the skin by means of wearable devices is of particular interest for the possibility to perform discreet and unobtrusive monitoring of BAC, but available devices are still lacking reliability, accuracy and sensitivity. In this context, Bioinspira, Inc. has developed a versatile bacteriophage-based, colorimetric sensor platform technology for gas sensing. The novel, in-house developed technology uses a self-assembling bacteriophage sensor array (M13 bacteriophage) that can be genetically engineered to bind to specific gas molecules and change its color pattern based on type of gas and concentration. By leveraging this innovative technology, through this SBIR phase I study Bioinspira will assess the feasibility of realizing a wearable Biomimetic Sensor for Transdermal Alcohol Monitoring (BiosTAM) for real-time monitoring of ethanol vapor that evaporates through the skin. Preliminary laboratory data showed the ability of BioInspira’s platform technology to detect ethanol. The development of a phage-based ethanol sensor with high will fill a technological gap in alcohol behavior monitoring, enabling more comprehensive and detailed medical studies, and will offer the possibility to monitor and manage personal alcohol use disorder. In SBIR Phase I, the technical feasibility of detecting ethanol vapor selectively, sensitively and real-time will be assessed. To this extent, the following R&D activities will be conducted: 1. Production and evaluation of gas phage array candidates for the selective and sensitive detection of ethanol vapor; 2. Calibration of the best performing phage sensor candidate for the detection of ethanol concentration under different temperature and relative humidity conditions. The results obtained will be preparatory for a SBIR Phase II, where the clinical trials will be performed for the correlation of transdermal alcohol measurements with BAC. Keywords: Alcoholism, Alcohol abuse, wearable biosensor, phage, perspired ethanol, alcohol vapor.