Biomimetic Wearable Sensors for Alcohol Monitoring

项目总结 根据2015年全国毒品使用与健康调查(NSDUH)，1510万成年人饮酒 美国的骚乱。酗酒已被确认为疾病、残疾和 死亡率，带来了巨大的经济和社会负担。实时、准确和谨慎的设备，用于 血液酒精浓度(BAC)的评估对于可靠地支持医学研究是必不可少的 监测酒精行为的可靠手段，以及有效的个人自我评估。测量 通过可穿戴设备通过皮肤出汗的乙醇尤其令人感兴趣，因为 对BAC进行谨慎和隐蔽的监控，但可用的设备仍然缺乏可靠性、准确性 和敏感。在此背景下，Biospirira，Inc.开发了一种基于噬菌体的多功能比色法 气体传感的传感器平台技术。这项新颖的内部开发技术使用了一种自组装技术 可通过基因工程与特定气体结合的噬菌体传感器阵列(M13噬菌体) 分子并根据气体类型和浓度改变其颜色模式。通过利用这一创新的 技术方面，通过此次SBIR阶段的研究，Biospirira将评估实现可穿戴的可行性 用于实时监测乙醇蒸气的仿生经皮酒精监测传感器(BiosTAM) 通过皮肤蒸发。初步实验室数据显示，BioInspira的平台技术 来检测乙醇。高效噬菌体乙醇传感器的研制将填补我国乙醇传感器的技术空白 酒精行为监测，使更全面和详细的医学研究，并将提供 监测和管理个人酒精使用障碍的可能性。在SBIR第一阶段， 对乙醇蒸气的选择性、灵敏和实时检测进行评估。从这个意义上讲，下面的研发 将开展的活动包括：1.筛选和鉴定噬菌体阵列候选毒株 对乙醇蒸气的灵敏检测；2.最佳性能噬菌体传感器候选的校准 检测不同温度和相对湿度条件下的乙醇浓度。结果是 获得的结果将为SBIR第二阶段做准备，在该阶段，将进行临床试验，以研究 用BAC进行酒精透皮吸收测量。 关键词：酒精中毒，酒精滥用，可穿戴式生物传感器，噬菌体，出汗乙醇，酒精蒸气。

PROJECT SUMMARY According to the 2015 National Survey of Drug Use and Health (NSDUH), 15.1 million adults had alcohol use disorder in the United States. Alcoholism has been identified as an important risk factor for illness, disability, and mortality, carrying a huge economic and societal burden. Real-time, accurate and discreet devices for the assessment of Blood Alcohol Concentration (BAC) are essential to empower medical research with reliable reliable means to monitor alcohol behavior, as well as for effective personal self-assessment. Measurement of ethanol perspired through the skin by means of wearable devices is of particular interest for the possibility to perform discreet and unobtrusive monitoring of BAC, but available devices are still lacking reliability, accuracy and sensitivity. In this context, Bioinspira, Inc. has developed a versatile bacteriophage-based, colorimetric sensor platform technology for gas sensing. The novel, in-house developed technology uses a self-assembling bacteriophage sensor array (M13 bacteriophage) that can be genetically engineered to bind to specific gas molecules and change its color pattern based on type of gas and concentration. By leveraging this innovative technology, through this SBIR phase I study Bioinspira will assess the feasibility of realizing a wearable Biomimetic Sensor for Transdermal Alcohol Monitoring (BiosTAM) for real-time monitoring of ethanol vapor that evaporates through the skin. Preliminary laboratory data showed the ability of BioInspira’s platform technology to detect ethanol. The development of a phage-based ethanol sensor with high will fill a technological gap in alcohol behavior monitoring, enabling more comprehensive and detailed medical studies, and will offer the possibility to monitor and manage personal alcohol use disorder. In SBIR Phase I, the technical feasibility of detecting ethanol vapor selectively, sensitively and real-time will be assessed. To this extent, the following R&D activities will be conducted: 1. Production and evaluation of gas phage array candidates for the selective and sensitive detection of ethanol vapor; 2. Calibration of the best performing phage sensor candidate for the detection of ethanol concentration under different temperature and relative humidity conditions. The results obtained will be preparatory for a SBIR Phase II, where the clinical trials will be performed for the correlation of transdermal alcohol measurements with BAC. Keywords: Alcoholism, Alcohol abuse, wearable biosensor, phage, perspired ethanol, alcohol vapor.