Core 1: Genomics and Data Management Core

项目总结 单细胞rna-seq和其他变革性基因组技术提供了将人类 免疫学到下一层次的细胞细节，定义单个细胞比 以前是有可能的。作为一种高度细胞异质性的疾病，系统性红斑狼疮的治疗时机已经成熟。 受单电池技术的影响。基因组学和数据管理核心(GDMC)将提供状态- 为拟议的狼疮中心提供最先进的基因组技术和所有数据管理能力 研究。GDMC的主要贡献将是提供和开发分析SLE的技术 患者样本采用单细胞分辨率。应用单细胞技术将有助于定义细胞类型 这些患者群体的特征，允许在患者和正常细胞之间进行特定的比较 并有可能定义与系统性红斑狼疮相关的新细胞类型和/或疾病的分子亚型。 单细胞rna-seq将被用作定义细胞群体和识别特定细胞类型的发现工具。 记号笔。流式细胞术和质量细胞术应用程序将利用这些信息来开发新的 可以比单细胞转录物(即在更多患者上)应用更大吞吐量的抗体 并且每个样本具有更多数量的细胞)。最终，这种细胞表型的发展 这种方法将使SLE患者的分层得到改善。此外，GDMC的目标是提供单曲 用于从功能上询问新的细胞群体和途径的细胞工具，这些细胞群体和途径由 单细胞转录，因此有可能发现SLE治疗的新策略。数据 GDMC的管理活动将确保以统一和强有力的方法进行实验设计和 对中心的两个研究项目进行了分析。GDMC将负责数据和信息流 负责中心所有组成部分，并负责提交完整的数据集/项目 到适当的公共档案馆。GDMC的具体目标是：1)提供最先进的单曲 细胞转录技术；2)提供最先进的细胞表型；以及3)提供中央 狼疮研究中心生成的数据的存储、管理和信息安全。其核心是 因此，将通过应用强大的 基因组技术与专家数据分析和管理支持相结合。

PROJECT SUMMARY Single cell RNA-seq and other transformative genomic technologies provide the opportunity to take human immunology to the next level of cellular detail, defining individual cells in greater orders of magnitude than previously possible. As a disease of great cellular heterogeneity, Systemic Lupus Erythematosus is ripe to be impacted by single cell technologies. The Genomics and Data Management Core (GDMC) will provide state- of-the-art genomics technologies and all data management capabilities for the proposed Center for Lupus Research. A major contribution of the GDMC will be to provide and develop the technology to analyze SLE patient samples with single cell resolution. Applying single cell technologies will help to define cell type characteristics in these patient populations, allow for specific comparisons between patient versus normal cell types and could potentially define new cell types associated with SLE and/or molecular subtypes of disease. Single cell RNA-seq will be used as a discovery tool to define cell populations and identify cell-type-specific markers. The flow and mass cytometry applications will utilize this information to develop new panels of antibodies that can be applied with greater throughput than single cell transcriptomics (i.e. on more patients and with greater numbers of cells per sample). Ultimately, the development of such a cellular phenotyping approach will enable improved stratification of SLE patients. In addition, the GDMC aims to provide the single cell tools to functionally interrogate new cell populations and pathways revealed by the resolving power of single cell transcriptomics, thus potentially uncovering new strategies for SLE therapeutics. The data management activities of the GDMC will ensure a unified and robust approach to experimental design and analysis for both research projects of the Center. GDMC will be responsible for the flow of data and information across all components of the Center and will be responsible for the submission of completed data sets/projects to the appropriate public archives. The Specific Aims for the GDMC are to 1) Provide state-of-the-art single cell transcriptomic technologies; 2) Provide state-of-the-art cellular phenotyping; and 3) Provide central storage, management and information security for data generated by the Center for Lupus Research. The core will thus fulfill essential scientific and translational needs across the Center through the application of powerful genomic technologies combined with expert data analysis and management support.