Pathogenesis of sepsis-induced dysfunction of innate immunity

我们的长期目标是研究脓毒症发病的分子机制，这是一种潜在的 由严重创伤和创伤感染引起的危及生命的疾病。败血症是最昂贵的 退伍军人医院需要治疗的情况。当脓毒症变得严重时，即使是在 适当的照顾。脓毒症与压倒性炎症反应和先天调节失调有关 免疫，这可能导致持续性炎症和多器官衰竭。实现这一目标的机制 发生的病理生理问题尚不清楚。有证据表明，巨噬细胞在 在先天免疫反应中的作用。它们会两极分化为M1表型(即炎症性 巨噬细胞)和M2表型(即抗炎巨噬细胞)依赖于局部炎症 环境。目前，脓毒症时炎症部位巨噬细胞的确切表型尚不清楚。 脓毒症中巨噬细胞极化的分子机制尚不清楚。此外， 巨噬细胞极化在脓毒症相关免疫失调发病机制及组织中的作用 受伤情况尚未得到澄清。因此，我们将重点填补这个项目中的这些知识空白。在……里面 初步研究发现，多菌败血症与M1样巨噬细胞增多有关。 小鼠的肺。M1巨噬细胞活化与细胞关键调节因子SIRT6水平的增加有关 新陈代谢。此外，功能丧失和功能获得研究揭示了SIRT6和M1之间的联系 巨噬细胞激活。因此，我们将研究SIRT6是否在败血症侮辱诱导中发挥重要作用 M1巨噬细胞活化和组织损伤，如果是这样，我们将研究SIRT6的分子机制 促进M1巨噬细胞活化。为此，我们将进行以下研究。具体目标1将 确定SIRT6在细胞因子刺激和脓毒症条件下M1巨噬细胞活化中的作用。 具体目标2将研究SIRT6如何在炎症中增强M1巨噬细胞的激活。具体目标3将 研究巨噬细胞中SIRT6相关信号轴是否影响脓毒症的发病机制。成就 将为脓毒症的严重炎症如何引起提供新的信息 M1-M2巨噬细胞激活失衡，最终可能导致制定治疗策略 脓毒症患者巨噬细胞稳态的维持。从拟议工作中得出的数据将 扩大我们对脓毒症发展为持续性炎症和 多器官损伤。脓毒症是外科病人常见和严重的并发症。 退伍军人事务部医疗保健系统。因此，这项提案的主题是及时重要的，并与 VA危重病人救治的临床应用。

Our long-term goal is to study molecular mechanisms underlying pathogenesis of sepsis, a potentially life-threatening illness caused by severe traumatic injury and trauma infections. Sepsis is the most costly condition for VA hospitals to treat. When sepsis becomes severe, it has a high mortality rate even with appropriate care. Sepsis is associated with overwhelming inflammatory response and dysregulation of innate immunity, which can lead to persistent inflammation and multiple organ failure. The mechanisms by which this pathophysiological problem occurs remain unknown. Evidence shows that macrophages play an important role in the innate immune response. They undergo polarization to M1 phenotype (i.e. inflammatory macrophages) and M2 phenotype (i.e. anti-inflammatory macrophages) in inflammation depending on local environments. Currently, the exact phenotype of macrophages in inflammatory sites during sepsis is not clear. The molecular mechanisms underlying polarization of macrophages in sepsis is unknown. Furthermore, the impact of macrophage polarization in pathogenesis of sepsis-associated immune dysregulation and tissue injury has not been elucidated. Thus, we will focus on filling these knowledge gaps in this project. In preliminary studies, we found that polymicrobial sepsis is associated with increase in M1-like macrophages in mouse lungs. M1 macrophage activation is coupled with increase in levels of Sirt6, a key regulator for cell metabolism. Furthermore, loss-of-function and gain-of-function studies revealed the link between Sirt6 and M1 macrophage activation. Therefore, we will study whether Sirt6 plays an important role in septic insult-induced M1 macrophage activation and tissue injury, and if so, we will study the molecular mechanism by which Sirt6 promotes M1 macrophage activation. To this end, we will execute the following studies. Specific Aim 1 will determine the role of Sirt6 in M1 macrophage activation under cytokine-stimulation and septic condition. Specific Aim 2 will study how Sirt6 potentiates M1 macrophage activation in inflammation. Specific Aim 3 will examine whether Sirt6-associated signal axis in macrophages impacts pathogenesis of sepsis. Achievement of these specific aims will provide novel information regarding how severe inflammation in sepsis causes imbalance of M1-M2 macrophage activation, which may ultimately lead to development of strategies for maintaining macrophage homeostasis in patients with sepsis. Data derived from the proposed work will expand our knowledge on mechanisms underlying progression of sepsis to persistent inflammation and multiple organ injury. Sepsis is a common and serious complication for surgery patients in the Department of Veterans Affairs Healthcare System. Thus, the subject matter of this proposal is timely important and links to clinical application for management of VA patients with critical illness.