Imaging, Protein Production, and Chemical Biology Core
Imaging, Protein Production, and Chemical Biology Core
批准号:
10161850
负责人:
Loren Gi Fong
金额:
$51.48万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-05-01 至 2024-04-30
关键词:
AlkynesAntibodiesAntibody FormationAzidesBindingBinding SitesBiologyBiomedical ResearchBiotinCellsChemicalsChemistryCholesterolCholesterol HomeostasisDiazomethaneDiseaseElectron MicroscopeElectron MicroscopyElectronsEnsureEnzyme-Linked Immunosorbent AssayEquipmentFab ImmunoglobulinsFluorescence MicroscopyFundingGoalsHealthHigh Density LipoproteinsHuman ResourcesImageInsectaInvestigationIsotopesLabelLightLipidsMass Spectrum AnalysisMicroscopyMovementNational Heart, Lung, and Blood InstitutePreparationProductionProductivityProteinsProteolysisProteomeProteomicsProtocols documentationReagentRecombinant AntibodyRecombinant ProteinsRegulationResearchResearch PersonnelSamplingScanningScanning Electron MicroscopyServicesSignal TransductionStable Isotope LabelingSterolsStructureSupervisionSystemTertiary Protein StructureTissuesTransmission Electron MicroscopyTriglyceride Metabolismactivity-based protein profilingbasecellular imagingcostcrosslinkcycloadditionequipment acquisitionexperiencehigh resolution imagingimaging modalityinstrumentlight microscopylipid transportmass spectrometermicroscopic imagingmutantnanomachinenovelnovel strategiesoperationprotein expressionrecruitsample collectionsample fixationtooltrafficking
中文摘要
核心:生物成像、蛋白质和生产、生物和化学、生物和核心技术。
摘要/摘要:
国际成像、蛋白质和生产、生物和化学、生物学和核心技术的目标是最大限度地促进科学和技术进步。
而提高PPG调查人员的生产率,主要是通过提供必要的技术服务和试剂来实现的。核心技术将主要表现在三个方面。
PPG的主要功能是:(1)提供先进的光学显微镜和电子显微镜成像;;(2)提供蛋白质和抗体。
生产;;和(3)化学和蛋白质组学。所有这三个PPG组件项目都将利用至少两项核心技术服务。
这些项目中的两个将不会全部利用这三个项目。如果将这些活动集中在一个核心项目内,将不会在2010年之前从新的PPG中受益。
提高生产效率,降低生产成本。核心设备将提供全方位的扫描和传输电子设备的服务。
显微技术、标准技术和活细胞技术、共聚焦技术、荧光技术、纳米技术和成像技术。后者是一种新的技术。
基于质谱学的生物成像技术使我们有可能创造出细胞和细胞的高分辨率图像。
组织是完全基于它们的同位素含量的。PPG的调查人员表示,他们将不再使用NanoSIMS技术来可视化和量化组织。
脂类在细胞和组织中的定位和运动方式。PPG的调查人员还将研究新的脂类分子。
调节血脂转运和信号转导的新机制,特别是几乎没有试剂存在的机制。需要帮助解决这些问题。
据调查,该核心项目将生产用于PPG临床研究的蛋白质和抗体试剂(例如,难溶的GPIHBP1)。
稳定的(同位素标记的)Alo-D4,(5D2)Fab(碎片)。最后,我们的核心将不会执行以活动为基础的化学。
蛋白质组学和蛋白质同位素谱是基于蛋白质水解酶活性的蛋白质谱分析。这是最先进的。
蛋白质组学的发现和平台将被用来帮助识别与高密度脂蛋白的主要细胞内转运相关的蛋白质--
派生出胆固醇,并提出了通过降低胆固醇来抑制信号传导的最新调控机制。对于未来五年来说,最核心的机制是。
凭借丰富的运营经验、强大的运营协议、团队和经验丰富的运营人员,我们将为PPG提供至关重要的运营服务。
调查人员。
英文摘要
Core A: Imaging, Protein Production, and Chemical Biology Core
SUMMARY/ABSTRACT
The goal of the Imaging, Protein Production, and Chemical Biology Core is to maximize the scientific progress
and productivity of PPG investigators by providing essential services and reagents. The core will perform three
major functions for the PPG: (1) advanced light and electron microscopy imaging;; (2) protein and antibody
production;; and (3) chemical proteomics. All three PPG component projects will utilize at least two core services,
and two of the projects will utilize all three. Centralizing these activities within a core will benefit the PPG by
increasing productivity and reducing costs. The core will provide full-service scanning and transmission electron
microscopy, standard and live-cell confocal fluorescence microscopy, and NanoSIMS imaging. The latter is a
mass spectrometry–based imaging modality that makes it possible to create high-resolution images of cells and
tissues based solely on their isotopic content. PPG investigators will use NanoSIMS to visualize and quantify the
localization and movement of lipids in cells and tissues. PPG investigators will also study new molecules and
novel mechanisms regulating lipid transport and signaling, for which few reagents exist. To assist these
investigations, the core will produce protein and antibody reagents for PPG studies (e.g., soluble GPIHBP1,
stable isotope–labeled ALO-D4, 5D2 Fab fragments). Finally, the core will perform Activity-Based Chemical
Proteomics and isotopic Tandem Orthogonal Proteolysis‒Activity-Based Protein Profiling. These state-of-the-art
proteomics discovery platforms will be used to identify proteins involved in the intracellular trafficking of HDL-
derived cholesterol and the regulation of STING signaling by cholesterol. For the next five years, the core is
poised—with the experience, robust protocols, and experienced personnel—to provide vital services to PPG
investigators.
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会议论文
Imaging, Protein Production, and Chemical Biology Core
-
批准号:10613966
-
项目类别:
-
资助金额:$51.48万
-
财政年份:2019
-
负责人:Loren Gi Fong
-
依托单位:
Imaging, Protein Production, and Chemical Biology Core
-
批准号:10397412
-
项目类别:
-
资助金额:$51.48万
-
财政年份:2019
-
负责人:Loren Gi Fong
-
依托单位:
Development of a New Therapeutic Approach for Prelamin A Diseases
-
批准号:9459813
-
项目类别:
-
资助金额:$31.57万
-
财政年份:2014
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负责人:Loren Gi Fong
-
依托单位:
Development of a New Therapeutic Approach for Prelamin A Diseases
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批准号:9027789
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项目类别:
-
资助金额:$31.57万
-
财政年份:2014
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负责人:Loren Gi Fong
-
依托单位:
Core A Biology and Antibody Core
-
批准号:7898774
-
项目类别:
-
资助金额:$47.24万
-
财政年份:2009
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负责人:Loren Gi Fong
-
依托单位:
Protein Prenylation and Progeria
-
批准号:8209225
-
项目类别:
-
资助金额:$38.12万
-
财政年份:2009
-
负责人:Loren Gi Fong
-
依托单位:
Protein Prenylation and Progeria
-
批准号:7581598
-
项目类别:
-
资助金额:$38.5万
-
财政年份:2009
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负责人:Loren Gi Fong
-
依托单位:
Protein Prenylation and Progeria
-
批准号:8011191
-
项目类别:
-
资助金额:$38.5万
-
财政年份:2009
-
负责人:Loren Gi Fong
-
依托单位:
Protein Prenylation and Progeria
-
批准号:7748017
-
项目类别:
-
资助金额:$38.5万
-
财政年份:2009
-
负责人:Loren Gi Fong
-
依托单位:
New Therapeutic Approaches for Hutchinson-Gilford Progeria Syndrome
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批准号:7839423
-
项目类别:
-
资助金额:$23.41万
-
财政年份:2009
-
负责人:Loren Gi Fong
-
依托单位:
Protein Prenylation and Progeria
-
批准号:8431778
-
项目类别:
-
资助金额:$36.29万
-
财政年份:2009
-
负责人:Loren Gi Fong
-
依托单位:
Core A Biology and Antibody Core
-
批准号:7537506
-
项目类别:
-
资助金额:$36.53万
-
财政年份:2008
-
负责人:Loren Gi Fong
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依托单位:
Mouse Model and Protein Expression Core
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批准号:8968256
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项目类别:
-
资助金额:$37.26万
-
财政年份:2008
-
负责人:Loren Gi Fong
-
依托单位:
New Therapeutic Approaches for Hutchinson-Gilford Progeria Syndrome
-
批准号:7743825
-
项目类别:
-
资助金额:$38.63万
-
财政年份:2007
-
负责人:Loren Gi Fong
-
依托单位:
New Therapeutic Approaches for Hutchinson-Gilford Progeria Syndrome
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批准号:7545489
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项目类别:
-
资助金额:$38.63万
-
财政年份:2007
-
负责人:Loren Gi Fong
-
依托单位:
New Therapeutic Approaches for Hutchinson-Gilford Progeria Syndrome
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批准号:7341662
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项目类别:
-
资助金额:$38.63万
-
财政年份:2007
-
负责人:Loren Gi Fong
-
依托单位:
New Therapeutic Approaches for Hutchinson-Gilford Progeria Syndrome
-
批准号:7179105
-
项目类别:
-
资助金额:$38.63万
-
财政年份:2007
-
负责人:Loren Gi Fong
-
依托单位:
Core A Biology and Antibody Core
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批准号:8378634
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项目类别:
-
资助金额:$46.13万
-
财政年份:--
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负责人:Loren Gi Fong
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依托单位:
Core A Biology and Antibody Core
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批准号:8298436
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项目类别:
-
资助金额:$46.51万
-
财政年份:--
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负责人:Loren Gi Fong
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依托单位:
Imaging, Protein Production, and Chemical Biology Core
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批准号:9919628
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项目类别:
-
资助金额:$51.48万
-
财政年份:--
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负责人:Loren Gi Fong
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依托单位:
海外基金