用于果蝇功能基因替换的综合人类 cDNA 文库-猫眼课题宝

用于果蝇功能基因替换的综合人类 cDNA 文库

批准号：

10167394

负责人：

HUGO J BELLEN

金额：

$75.03万

依托单位：

BAYLOR COLLEGE OF MEDICINE

依托单位国家：

美国

项目类别：

财政年份：

2016

资助国家：

美国

项目状态：

已结题

起止时间：

2016-06-01 至 2024-05-31

来源：

https://reporter.nih.gov/project-details/10167394

关键词：

2019-nCoV Affect Animal Model Animals Award Bacterial Artificial Chromosomes Binding Biological COVID-19 COVID-19 pandemic Cells Code Collection Communities Complementary DNA Coronavirus Data Disease Drops Drosophila genus Drosophila melanogaster FlyBase Gene Transfer Techniques Genes Genetic Genetic Models Genome Human Human Genome Individual Infection Injections Knowledge Libraries Membrane Proteins Microcephaly Molecular Mutagenesis Mutation Names Nonstructural Protein Nucleocapsid Pathogenesis Pathogenicity Pattern Phenotype Plasmids Play Proteins Publications RNA RNA Interference Rare Diseases Reagent Research Personnel Resources Role System Technology Tissues Transgenic Organisms Update Variant Viral Viral Genome Viral Proteins Virus Replication Work ZIKV infection base cDNA Library fly gene function gene replacement human disease in vivo infection risk innovation mutant novel novel coronavirus online community pandemic disease parent grant protein function screening site-specific integration success tool vector

项目摘要

项目成果

HUGO J BELLEN的其他基金

相似基金

相关文献

中文摘要

翻译

项目总结最近由于感染SARS-CoV-2冠状病毒而导致的新冠肺炎大流行创造了一个紧急的需要了解这种新疾病发病的生物学机制。SARS-CoV-2是一种冠状病毒，其RNA基因组略低于30kb，编码26种相互作用的蛋白质与数百种人类蛋白质结合，并启动一系列致病步骤。在这里，我们使用了基因以黑腹果蝇为模型，了解新冠肺炎参与的基因和蛋白质功能。我们会建立UAS-cDNA库和转基因果蝇以表达和功能评估病毒蛋白质。我们还将为与病毒有关的人类基因组中的300多个基因培育转基因果蝇复制与新冠肺炎病。我们将为这些果蝇的同源物制作果蝇试剂利用独特的人类基因外挂技术，我们将更新网上传播枢纽这些资源被提供给世界各地的果蝇实验室。

英文摘要

PROJECT SUMMARY The recent pandemic of COVID-19 due to infection with the SARS-CoV-2 coronavirus has created an urgent need to understand the biological mechanisms of pathogenesis of this new disease. The SARS-CoV-2 is a coronavirus with a slightly less than 30 kilobases (kb) long RNA genome that encodes 26 proteins that interact with several hundred human proteins and initiate a number of pathogenic steps. Here we use the genetic model Drosophila melanogaster to understand gene and protein function involved in COVID-19. We will generate a library of UAS-cDNA constructs and transgenic flies to express and functionally assess the viral proteins. We will also generate transgenic flies for over 300 genes in the human genome involved in viral replication and COVID-19 disease. We will generate Drosophila reagents for the Drosophila homologs of these human genes using a unique drop-in technology and we will update the online hub for the dissemination of these resources to Drosophila labs throughout the world.

期刊论文(0)

专著(0)

科研奖励(0)

会议论文