Physiology Core

生理学核心

• 批准号: 10170321
• 负责人: Orson W Moe
• 金额: $ 26.17万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10170321
• 关键词: Agreement; Animals; Authorship; Biological Assay; Blood Pressure; Blood gas; Capillary Electrophoresis; Charge; Chemistry; Chronic Kidney Failure; Citrates; Collaborations; Communities; Creatinine; Cultured Cells; Development; Diagnosis; Disease; Electrolytes; Electrophysiology (science); Enzyme-Linked Immunosorbent Assay; Equipment; Equus caballus; Fee-for-Service Plans; Functional disorder; Gene Expression; Gene Proteins; Generations; Genetic; Goals; Immunoprecipitation; In Vitro; Individual; Ion-Selective Electrodes; Joints; Kidney; Kidney Diseases; Knowledge Discovery; Laboratories; Measurement; Metabolic; Mission; Mus; Mutant Strains Mice; Organ; Paper; Phenotype; Physiological; Physiology; Polymerase Chain Reaction; Production; Quality Control; Radio; Rattus; Recording of previous events; Recovery; Renal function; Renal tubule structure; Research; Research Personnel; Resources; Rodent; Role; Sampling; Science; Scientist; Serum; Services; Stains; Standardization; Tail; Techniques; Telemetry; Testing; Time; Training; Training and Education; Urine; Validation; Visit; Work; case-by-case basis; cost; flame photometry; improved; nanolitre; nephrogenesis; novel; patch clamp; tool

PROJECT SUMMARY/ABSTRACT Rodents are important tools for studying kidney function and diseases. However, expertise and equipment for physiological analysis of kidney function in mice are not readily available to many investigators in the renal research community, and even less so outside the renal community. Furthermore, production of mutant mice has allowed many investigators outside the renal community to uncover unexpected renal phenotypes and venture into renal research. The objective of the Physiology Core is to assist investigators with a wide range of physiological techniques to study kidney physiology and diseases at the cellular, organ, and importantly, whole animal level. This Core serves the three major themes of the O'Brien Center of Renal Development and Genetics, Physiology and Pathophysiology, Chronic Kidney Disease and tis Complications. To achieve these objectives, the Core will provide specific assistances: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. Whole animal metabolic and clearance studies Measurement of blood pressure by tail cuff or telemetry Measurements of serum and urine creatinine and citrate Measurement of serum and urine electrolytes and chemistry Serum or urine soluble Klotho by our own immunoprecipitation-immunoblot Electrolytes in nanolitre volume using ion-selective electrodes Isolation of individual tubules for real-time PCR and immuno-fluorescent staining In vitro microperfusion of individually isolated tubules Electro-physiological studies Participation of an exploratory Klotho ELISA an Alpha LISA 1 to 5 are offered as services with nominal non-profit cost recovery charges. 6 to 9 are offered as collaborations with mutual agreement between Investigators and Core B Directors with clearly defined deliverables, no cost, but with joint authorships. 10 is a new exploratory techniques where investigators can work with our Core Directors to co-develop these new assays by contributing samples, scientific, or technical input. There will be no charge and any resultant paper (no guaranteed deliverables) will be co-authored. Surveillance is in place to evaluate efficiency and to maintain quality control of services provided. The Core will also provide education and training to the investigators and their staff and trainees. The Core Directors have a track record of providing assistance and training to visiting scientists locally and nationally. The Physiology Core Kidney contributes to the understanding of themes of the O'Brien Center in kidney development and genetic, renal physiology and pathophysiology, and chronic kidney disease, and our long-term goal of improving the diagnosis and treatment of kidney diseases.

项目总结/摘要 啮齿动物是研究肾功能和疾病的重要工具。然而，专业知识和设备， 小鼠肾功能的生理学分析对于许多肾功能研究者来说是不容易获得的。 研究社区，甚至更少，所以在肾脏社区以外。此外，突变小鼠的生产 使许多肾脏领域以外的研究者能够发现意想不到的肾脏表型， 进行肾脏研究生理学核心的目标是帮助研究人员进行广泛的 生理技术，研究肾脏生理和疾病的细胞，器官，重要的是，整体 动物水平。该核心服务于奥布莱恩肾脏发育和遗传学中心的三大主题， 生理学和病理生理学，慢性肾脏疾病及其并发症。 为实现这些目标，核心将提供具体援助： 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 全动物代谢和清除研究 通过尾套或遥测测量血压 血清和尿肌酐和柠檬酸盐的测量 血清和尿液电解质和化学测量 通过我们自己的免疫沉淀-免疫印迹法检测血清或尿液中可溶性Klotho 使用离子选择性电极的纳升体积的电解质 分离单个小管用于实时PCR和免疫荧光染色 单个分离的肾小管的体外微灌注 电生理研究 参与探索性Klotho ELISA和Alpha丽莎 1至5是作为服务提供的，收取象征性的非盈利成本回收费用。6至9个作为合作提供 经研究者和核心B总监相互同意，明确定义可交付成果，无费用， 而是与他人合著。10是一种新的探索性技术，研究人员可以与我们的核心 董事通过提供样本、科学或技术投入共同开发这些新的检测方法。不会有 收费和任何由此产生的文件（没有保证交付）将共同撰写。监控已经到位， 评估效率，并保持所提供服务的质量控制。 核心小组还将向调查员及其工作人员和受训人员提供教育和培训。核心 董事们有向当地和全国的访问科学家提供援助和培训的记录。 肾脏生理学核心有助于理解奥布莱恩中心在肾脏方面的主题 发展和遗传，肾脏生理学和病理生理学，慢性肾脏疾病，以及我们的长期 目的是提高肾脏疾病的诊治水平。