NH PHL Discipline A Microbiology Analytical Track 4 - Whole Genome Sequencing

学科A：微生物学 分析途径4：全基因组测序 通过捕获当前和不断发展的基因组多样性来增强GenomeTrakr网络 在人类或动物食品，环境， 和水样。通过这一分析轨道产生的数据将存放在 国家生物技术信息中心（NCBI）的公共数据库，可以评估 由研究人员和公共卫生官员进行真实的时间比较和分析， 加快食源性疾病暴发调查，减少食源性疾病和死亡。 NH PHL将对各种沙门氏菌、单核细胞增生李斯特菌、滋贺毒素- 产生E.大肠杆菌（STEC）和其他食源性相关细菌、病毒和寄生虫病原体 隔离，并提供任何要求的培训和指导其他国家实验室。的NH PHL将保留完成本合同项下拟议工作所需的设施和人员。 项目所有WGS结果和元数据将与NCBI-NIH实时共享， 国家肠道病原体基因组数据库，与FDA CFSAN协调。关于PHL 将参加GenomeTrakr网络，包括会议，电话会议，熟练 测试和其他活动。NH PHL将根据需要参与多实验室验证研究 并至少有一名关键人员参加年度GenomeTrakr和LFFM面对面 会议. NH PHL将参加并通过年度能力测试，参加每月电话会议，维护ISO 17025认证，维护完成拟议工作所需的设施和人员， 并提供所有收集和分析的样本的详细季度摘要。关于PHL 将与FDA保持有效的20.88协议。

Discipline A: Microbiology Analytical Track 4: Whole Genome Sequencing To enhance the GenomeTrakr network by capturing the current and evolving genomic diversity of non-clinical pathogens across FDA-relevant areas in human or animal food, environmental, and water samples. The data generated through this analytical track, which will be housed in public databases at the National Center for Biotechnology information (NCBI), can be assessed by researchers and public health officials for real time comparison and analysis that promises to speed foodborne illness outbreak investigations and reduce foodborne illness and death. The NH PHL will sequence a variety of Salmonella, Listeria monocytogenes, Shiga toxin- producing E. coli (STEC) and other foodborne related bacterial, viral and parasite pathogen isolates, and provide any requested training and mentorship to other state laboratories. The NH PHL will maintain facilities and personnel necessary to complete the work proposed under this project. All WGS results and metadata will be shared in real-time with the NCBI-NIH curated national database for enteric pathogen genomes, in coordination with FDA CFSAN. The NH PHL will participate in the GenomeTrakr network, including meetings, conference calls, proficiency tests and other activities. The NH PHL will participate in multi-lab validation studies, as needed and have at least one key personnel attend the annual GenomeTrakr and LFFM Face-to-Face Meetings. NH PHL will participate and pass annual proficiency tests, attend monthly calls, maintain ISO 17025 accreditation, maintain facilities and personnel necessary to complete the work proposed, and provide a detailed quarterly summary of all samples collected and analyzed. The NH PHL will maintain a valid 20.88 agreement with the FDA.